SDF 1 Antibody - #AF5166

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产品: SDF 1 抗体
货号: AF5166
描述: Rabbit polyclonal antibody to SDF 1
应用: WB IHC
文献验证: WB, IHC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Dog, Chicken, Xenopus
蛋白号: P48061
RRID: AB_2837652

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 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
SDF 1 Antibody detects endogenous levels of total SDF 1.
RRID:
AB_2837652
引用格式: Affinity Biosciences Cat# AF5166, RRID:AB_2837652.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

12-O-tetradecanoylphorbol 13-acetate repressed protein 1; AI174028; C-X-C motif chemokine 12; Chemokine (C-X-C motif) ligand 12 (stromal cell-derived factor 1); Chemokine (C-X-C motif) ligand 12; Chemokine CXC motif ligand 12; cxcl12; hIRH; hSDF-1; Intercrine reduced in hepatomas; IRH; OTTHUMP00000019491; PBSF; Pre-B cell growth-stimulating factor; SCYB12; SDF 1; SDF-1; SDF-1-alpha(3-67); SDF-1a; SDF-1b; SDF1_HUMAN; SDF1A; SDF1B; Stromal cell-derived factor 1; Stromal cell-derived factor 1 delta; Stromal cell-derived factor 1 gamma; Stromal cell-derived factor 1a; Stromal cell-derived factor-1 alpha; Thymic lymphoma cell-stimulating factor; Tlsf; TLSF-a; TLSF-b; Tlsfa; Tlsfb; TPAR1;

抗原和靶标

免疫原:

A synthesized peptide derived from human SDF 1, corresponding to a region within C-terminal amino acids.

基因/基因ID:
CXCL12(6387)
描述:
Chemoattractant active on T-lymphocytes, monocytes, but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. Also binds to atypical chemokine receptor ACKR3, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. SDF-1-beta(3-72) and SDF-1-alpha(3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3-67) and thus to preserve activity on local sites. Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase.

研究领域

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction.   (View pathway)

· Environmental Information Processing > Signal transduction > NF-kappa B signaling pathway.   (View pathway)

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Immune diseases > Rheumatoid arthritis.

· Organismal Systems > Immune system > Chemokine signaling pathway.   (View pathway)

· Organismal Systems > Development > Axon guidance.   (View pathway)

· Organismal Systems > Immune system > Leukocyte transendothelial migration.   (View pathway)

· Organismal Systems > Immune system > Intestinal immune network for IgA production.   (View pathway)

文献引用

1). Highly efficient local delivery of endothelial progenitor cells significantly potentiates angiogenesis and full-thickness wound healing. Acta Biomaterialia, 2018 (PubMed: 29397318) [IF=9.4]

Application: WB    Species: rat    Sample: EPCs

Fig. 2 | CPB scaffolds activate the HIF-1 a /VEGF/SDF-1 a signaling pathway in EPCs. (A) Western blot analysis and quantification of Hif-1 a , VEGF, and SDF-1 a proteins in EPCs cultured on scaffolds for 3 days; (B) fluorescent images of F-actin (red) and Hif-1 a (green) in EPCs cultured on scaffolds for 3 days; (C) quantification of the cellular immunofluorescence intensity of Hif-1 a ; Scale bar are 20 l m, n = 3 independent experiments.
2). CircNf1-mediated CXCL12 expression in the spinal cord contributes to morphine analgesic tolerance. Brain, Behavior, and Immunity, 2023 (PubMed: 36202171) [IF=8.8]
3). Silencing c-Myc Enhances the Antitumor Activity of Bufalin by Suppressing the HIF-1α/SDF-1/CXCR4 Pathway in Pancreatic Cancer Cells. Frontiers in Pharmacology, 2020 (PubMed: 32362830) [IF=5.6]

Application: WB    Species: Human    Sample: pancreatic cancer cells

Figure 6 Downregulation of c-Myc enhanced the antitumor effect of bufalin in pancreatic cancer cells through the HIF-1α/SDF-1/CXCR4 pathway. (A) The protein expression of c-Myc, vimentin, E-cadherin, HIF-1α, CXCR4, and SDF-1 in PANC-1 and SW1990 pancreatic cancer cells under different treatments was detected via western blot. (B) Quantification results of protein expressions of c-Myc, vimentin, E-cadherin, HIF-1α, CXCR4, and SDF-1 in PANC-1 pancreatic cancer cells. (C) Quantification results of protein expression of c-Myc, vimentin, E-cadherin, HIF-1α, CXCR4, and SDF-1 in SW1990 pancreatic cancer cells (* p < 0.05, ** p < 0.01 vs control, ▲ p < 0.05, ▲▲ p < 0.01 vs bufalin treatment group, n = 3).
4). Injectable Silk Nanofiber Hydrogels for Sustained Release of Small-Molecule Drugs and Vascularization. ACS Biomaterials Science & Engineering, 2019 (PubMed: 33448809) [IF=5.4]
5). Kangfuxin Accelerates Extraction Socket Healing by Promoting Angiogenesis Via Upregulation of CCL2 in Stem Cells. Journal of Bone and Mineral Research, 2023 (PubMed: 37221128) [IF=5.1]
6). Chronic Intermittent Hypobaric Hypoxia Enhances Bone Fracture Healing. Frontiers in Endocrinology, 2021 (PubMed: 33664707) [IF=3.9]

Application: WB    Species: Rat    Sample: CIHH rats

Figure 5 The expression of SDF-1 and CXCR4 axis at fracture sites. (A) Western blot images of SDF-1, CXCR4, and β-actin in the callus area. (B) Quantitative real-time PCR of the callus SDF-1α. (C) Quantitative real-time PCR of the callus CXCR4. n=6, Mean ± SD, *p < 0.05, **p < 0.01, ***p < 0.001.
7). The protective effects of umbilical cord‐derived endothelial colony forming cells on hepatic veno‐occlusive disease. CELL BIOLOGY INTERNATIONAL, 2020 (PubMed: 32876991) [IF=3.3]
8). Zoledronic acid generates a spatiotemporal effect to attenuate osteoarthritis by inhibiting potential Wnt5a-associated abnormal subchondral bone resorption. PLOS ONE, 2021 (PubMed: 35900969) [IF=2.9]

Application: IHC    Species: Rat    Sample:

Fig 7 ZOL attenuates the expression of Wnt5a and osteoclastogenesis genes 4 weeks after DMM-induced OA. Expression of (A) Wnt5a, (B) CXCL12 and (C) NFATc1 determined by immunohistochemical staining and by (E) quantitative analysis. Scale bar, 200 μm. n = 6 per group. (D) Western blots and (F) quantitation of Wnt5a, RANKL, CXCL12 and NFATc1. Reverse transcription quantitative polymerase chain reaction of (G) Wnt5a, CXCL12, NFATc1, (H) RANKL, OPG, RANKL/OPGand (I) TRAP, Ctsk. n = 3 per group. *P < 0.05, **P < 0.01, and ***P < 0.001, compared with sham+PBS group; #P < 0.05, ##P < 0.01, and ###P < 0.001, compared with DMM+PBS group.

Application: WB    Species: Rat    Sample:

Fig 7 ZOL attenuates the expression of Wnt5a and osteoclastogenesis genes 4 weeks after DMM-induced OA. Expression of (A) Wnt5a, (B) CXCL12 and (C) NFATc1 determined by immunohistochemical staining and by (E) quantitative analysis. Scale bar, 200 μm. n = 6 per group. (D) Western blots and (F) quantitation of Wnt5a, RANKL, CXCL12 and NFATc1. Reverse transcription quantitative polymerase chain reaction of (G) Wnt5a, CXCL12, NFATc1, (H) RANKL, OPG, RANKL/OPGand (I) TRAP, Ctsk. n = 3 per group. *P < 0.05, **P < 0.01, and ***P < 0.001, compared with sham+PBS group; #P < 0.05, ##P < 0.01, and ###P < 0.001, compared with DMM+PBS group.

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