SMAD6 Antibody - #AF5160

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产品: SMAD6 抗体
货号: AF5160
描述: Rabbit polyclonal antibody to SMAD6
应用: WB IHC
文献验证: WB
反应: Human, Mouse
预测: Pig, Bovine, Horse, Sheep, Dog, Chicken
蛋白号: O43541
RRID: AB_2837646

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
HPLC Report
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse
克隆:
Polyclonal
特异性:
SMAD6 Antibody detects endogenous levels of total SMAD6.
RRID:
AB_2837646
引用格式: Affinity Biosciences Cat# AF5160, RRID:AB_2837646.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

hSMAD6; MAD homolog 6; MADH6; MADH7; Mothers against decapentaplegic homolog 6; Mothers against DPP homolog 6; SMAD 6; SMAD family member 6; SMAD mothers against DPP homolog 6; Smad6; SMAD6_HUMAN;

抗原和靶标

免疫原:

A synthesized peptide derived from human SMAD6, corresponding to a region within the internal amino acids.

基因/基因ID:
SMAD6(4091)
描述:
Acts as a mediator of TGF-beta and BMP antiflammatory activity. Suppresses IL1R-TLR signaling through its direct interaction with PEL1, preventing NF-kappa-B activation, nuclear transport and NF-kappa-B-mediated expression of proinflammatory genes. May block the BMP-SMAD1 signaling pathway by competing with SMAD4 for receptor-activated SMAD1-binding. Binds to regulatory elements in target promoter regions

研究领域

· Environmental Information Processing > Signal transduction > TGF-beta signaling pathway.   (View pathway)

文献引用

1). SENP3 deletion promotes M2 macrophage polarization and accelerates wound healing through smad6/IκB/p65 signaling pathway. Heliyon, 2023 (PubMed: 37180935) [IF=4.0]

Application: WB    Species: Mouse    Sample: macrophages

Fig. 5 SENP3 loss activated Smad6 signaling in macrophages. (A) An enrichment of wound healing and angiogenesis was negatively regulated in macrophages with SENP3fl/fl. (B) RNA-sequencing data analysis revealed that Smad6 and IκBα (Nfkbia) expression was significantly increased in macrophages with SENP3 knockdown. (C) Functional annotation analysis of genes with ectopic expression between macrophages with or without SENP3 knockdown. (D–F) RT‒qPCR and Western blot analysis were performed to evaluate Smad6 and IκBα expression in macrophages with or without SENP3 knockout. (G) Western blot analysis of Smad7 and TGFβ3. (H–I) Statistical analysis. Data were presented as mean ± s.d. *, P < 0.05; **, P < 0.01. SENP3, SUMO-specific protease 3; IκB, inhibitor of NF-κB; RT‒qPCR, real-time polymerase chain reaction.
2). Shh Protects the Injured Spinal Cord in Mice by Promoting the Proliferation and Inhibiting the Apoptosis of Nerve Cells via the Gli1-TGF-β1/ERK Axis. Cell biochemistry and function, 2025 (PubMed: 39751786) [IF=3.6]

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