Annexin A1 Antibody - #AF5154

Figure 4. Intestinal barrier permeability and intestinal-barrier-related protein expression. N = 8. (A) Serum diamine oxidase activity and (B) D-lactate contents. (C–F) The relative protein expression of ZO-1, Occludin, and MUC2 in the jejunum. CON, basal diet; EQ, basal diet + 200 g/ton ethoxyquin; AC, basal diet + 200 g/ton antioxidant compound. Abbreviations: DAO, diamine oxidase. ab Different superscript letters indicate significant differences (p < 0.05).

Fig. 3. Allopurinol upregulates junctional protein expression by suppressing TLR4/P65. (A–C) Representative immunoblotting of TLR4 (A), P65 (B) and IL-1β (C) from isolated colon after treatment with allopurinol or antibiotic. (D–F) Relative expression levels of Occludin (D), ZO-1 (E) and Claudin1 (F) genes were determined by Q-PCR from isolated colon after treatment with allopurinol or antibiotic. n = 6 mice per group. (G and H) Representative immunoblotting of Occludin, Claudin1（G）and IL-1β（H）from isolated colon after treatment with allopurinol or antibiotic. NC, control group; HY, hyperuricemia mice. Bars and error bars represent the Means ± SEM. *p < 0.05, **p < 0.01 verse Con, ##p < 0.01 verse HY using one-way ANOVA followed by Tukey's multiple comparison post-test.