ZO 1 Antibody - #AF5145

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产品: ZO 1 抗体
货号: AF5145
描述: Rabbit polyclonal antibody to ZO 1
应用: WB IHC IF/ICC
文献验证: WB, IHC, IF/ICC
反应: Human, Mouse, Rat, Pig, Monkey
预测: Horse, Dog
蛋白号: Q07157
RRID: AB_2837631

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 50ul RMB¥ 1000 1250 现货
 100ul RMB¥ 1840 2300 现货
 200ul RMB¥ 3000 现货

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实验方案
WB Handbook
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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat, Pig, Monkey
克隆:
Polyclonal
特异性:
ZO 1 Antibody detects endogenous levels of total ZO 1.
RRID:
AB_2837631
引用格式: Affinity Biosciences Cat# AF5145, RRID:AB_2837631.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Tight junction protein 1; Tight junction protein ZO-1; Tight junction protein ZO1; TJP1; zo-1; Zo1; ZO1_HUMAN; Zona occludens 1; Zona occludens 1 protein; Zona occludens protein 1; Zonula occludens 1 protein; Zonula occludens protein 1;

抗原和靶标

免疫原:

A synthesized peptide derived from human ZO 1, corresponding to a region within the internal amino acids.

基因/基因ID:
TJP1(7082)
描述:
The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions.

研究领域

· Cellular Processes > Cellular community - eukaryotes > Adherens junction.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Tight junction.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Gap junction.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Vibrio cholerae infection.

· Human Diseases > Infectious diseases: Bacterial > Epithelial cell signaling in Helicobacter pylori infection.

· Human Diseases > Infectious diseases: Bacterial > Salmonella infection.

文献引用

1). A Nucleophilicity-Engineered DNA Ligation Blockade Nanoradiosensitizer Induces Irreversible DNA Damage to Overcome Cancer Radioresistance. Advanced materials (Deerfield Beach, Fla.), 2024 (PubMed: 39246208) [IF=27.4]
2). Enhancing phage therapy by coating single bacteriophage-infected bacteria with polymer to preserve phage vitality. Nature biomedical engineering, 2025 (PubMed: 40000896) [IF=26.8]
3). Reinforcement of the intestinal mucosal barrier via mucus-penetrating PEGylated bacteria. Nature biomedical engineering, 2024 (PubMed: 38839928) [IF=26.8]
4). Dietary fiber alleviates alcoholic liver injury via Bacteroides acidifaciens and subsequent ammonia detoxification. Cell host & microbe, 2024 (PubMed: 38959900) [IF=20.6]
5). Restoring mucosal barrier homeostasis by in situ formation of a living-synthetic therapeutic coating. Nature communications, 2025 (PubMed: 40825983) [IF=16.6]

Application: IF/ICC    Species: Mouse    Sample:

Fig. 4: Restoration of the mucosal physicochemical barrier. A Images of AB staining of LPS-stimulated HT29-MTX-E12 cells incubated with PBS or 0.2 mg/ml Bi(NO3)3·5H2O for 12 h. B TEER data of LPS-stimulated epithelial cell monolayer in transwell insert after incubation with PBS or 3 μg/ml Bi(NO3)3·5H2O for 24 h (n = 3 independent samples). C Concentrations of FITC-dextran in the basolateral medium of transwell measured by a microplate fluorometer after incubation with PBS or 3 μg/ml Bi(NO3)3·5H2O for 4 or 12 h (n = 3 independent samples). Control group indicates unstimulated cells in (A–C). D-H Evaluation of vaginal barrier integrity of S. aureus-infected mice after intravaginal treatment with PBS, LGG, EG-Bi, or EG-Bi-LGG every day for 7 days. D Images of AF488-WGA (red) staining of vaginal sections from treated mice. Blue indicates cell nuclei stained with 4’,6-diamidino-2-phenylindole (DAPI). E Average fluorescence intensity of AF488-WGA of vaginal sections from D (n = 4 independent samples). F Immunofluorescence images of vaginal occludin (green) and ZO-1 (red) expressions from treated mice. Blue indicates cell nuclei stained with DAPI. Average fluorescence intensities of occludin (G) and ZO-1 (H) of vaginal sections from (F) (n = 3 independent samples). Data are mean ± SD. Statistical analysis was performed using one-way ANOVA with Fisher’s LSD multiple comparisons test, and p-values were indicated. Source data are provided as a Source Data file.
6). Chemical reaction-mediated covalent localization of bacteria. Nature Communications, 2022 (PubMed: 36528693) [IF=16.6]
7). Targeting P2Y14R protects against necroptosis of intestinal epithelial cells through PKA/CREB/RIPK1 axis in ulcerative colitis. Nature communications, 2024 (PubMed: 38453952) [IF=16.6]
8). Novel Role of Gut-Derived Roseburia Intestinalis in Safeguarding Intestinal Barrier Integrity and Microenvironment Homeostasis During Arsenic Exposure. Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2025 (PubMed: 40831218) [IF=15.1]

Application: WB    Species: Mouse    Sample:

Figure 1 Arsenic disrupts the mechanical barrier of intestine in the mice. A) Representative hematoxylin and eosin (H&E) stained images of intestine tissues. Scale bars, 100 µm (top) and 50 µm (bottom). B–D) Total pathological score, smooth muscle thickness, and mucosa depth of the intestine tissues (n = 3). E) Representative Alcian Blue-Periodic Acid-Schiff (AB-PAS) stained images of intestine tissues. Scale bars, 100 µm (top) and 50 µm (bottom). F) The number of goblet cells of the intestine tissues (n = 3). G,H) The mRNA expressions of Tjp-1 and occludin in intestines of mice (n = 3). I–K) Representative western blot images and statistical analyses of ZO-1 and Occludin in intestines of mice (n = 2).
9). The role of the microbiota–gut–brain axis in methamphetamine-induced neurotoxicity: Disruption of microbial composition and short-chain fatty acid metabolism. Acta Pharmaceutica Sinica B, 2024 (PubMed: 39664442) [IF=14.7]
10). Phytosphingosine contributes gut microbiota metabolism to alleviate low-grade endotoxemia-induced mastitis.. Microbiome, 2025 [IF=13.5]
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