ZO 1 Antibody - #AF5145

>>买2送1 进行中>>
>>赠JD卡 进行中>>
(230)   (88)  
产品: ZO 1 抗体
货号: AF5145
描述: Rabbit polyclonal antibody to ZO 1
应用: WB IHC IF/ICC
文献验证: WB, IHC, IF/ICC
反应: Human, Mouse, Rat, Pig, Monkey
预测: Horse, Dog
蛋白号: Q07157
RRID: AB_2837631

浏览相似产品>>

   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

联系销售
相关下载
MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat, Pig, Monkey
克隆:
Polyclonal
特异性:
ZO 1 Antibody detects endogenous levels of total ZO 1.
RRID:
AB_2837631
引用格式: Affinity Biosciences Cat# AF5145, RRID:AB_2837631.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Tight junction protein 1; Tight junction protein ZO-1; Tight junction protein ZO1; TJP1; zo-1; Zo1; ZO1_HUMAN; Zona occludens 1; Zona occludens 1 protein; Zona occludens protein 1; Zonula occludens 1 protein; Zonula occludens protein 1;

抗原和靶标

免疫原:

A synthesized peptide derived from human ZO 1, corresponding to a region within the internal amino acids.

基因/基因ID:
TJP1(7082)
描述:
The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions.

研究领域

· Cellular Processes > Cellular community - eukaryotes > Adherens junction.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Tight junction.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Gap junction.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Vibrio cholerae infection.

· Human Diseases > Infectious diseases: Bacterial > Epithelial cell signaling in Helicobacter pylori infection.

· Human Diseases > Infectious diseases: Bacterial > Salmonella infection.

文献引用

1). A Nucleophilicity-Engineered DNA Ligation Blockade Nanoradiosensitizer Induces Irreversible DNA Damage to Overcome Cancer Radioresistance. Advanced materials (Deerfield Beach, Fla.), 2024 (PubMed: 39246208) [IF=27.4]
2). Reinforcement of the intestinal mucosal barrier via mucus-penetrating PEGylated bacteria. Nature biomedical engineering, 2024 (PubMed: 38839928) [IF=26.8]
3). Enhancing phage therapy by coating single bacteriophage-infected bacteria with polymer to preserve phage vitality. Nature biomedical engineering, 2025 (PubMed: 40000896) [IF=26.8]
4). Dietary fiber alleviates alcoholic liver injury via Bacteroides acidifaciens and subsequent ammonia detoxification. Cell host & microbe, 2024 (PubMed: 38959900) [IF=20.6]
5). Targeting P2Y14R protects against necroptosis of intestinal epithelial cells through PKA/CREB/RIPK1 axis in ulcerative colitis. Nature communications, 2024 (PubMed: 38453952) [IF=16.6]
6). Chemical reaction-mediated covalent localization of bacteria. Nature Communications, 2022 (PubMed: 36528693) [IF=16.6]
7). The role of the microbiota–gut–brain axis in methamphetamine-induced neurotoxicity: Disruption of microbial composition and short-chain fatty acid metabolism. Acta Pharmaceutica Sinica B, 2024 (PubMed: 39664442) [IF=14.7]
8). Extracellular vesicles of Fusobacterium nucleatum compromise intestinal barrier through targeting RIPK1-mediated cell death pathway. Gut Microbes, 2021 (PubMed: 33769187) [IF=12.2]

Application: WB    Species: human    Sample: Caco2 cells

Figure 2. |FnEVs promote TNF-α induced Caco2 cells death and barrier loss.(i) Immunofluorescence results of immunofluorescence localization of the TJ protein ZO-1(red, ZO-1 positive; blue, DAPI) and immunoblot analysis of ZO-1 protein. Scale bar = 50 um. *p < .05, **p < .01, ***p < .001. All the results were repeated three times. All data were presented as means ± SD (n = 3). TEER, transepithelial electrical resistance measurements.

Application: IF/ICC    Species: human    Sample: Caco2 cells

Figure 2. |FnEVs promote TNF-α induced Caco2 cells death and barrier loss.(i) Immunofluorescence results of immunofluorescence localization of the TJ protein ZO-1(red, ZO-1 positive; blue, DAPI) and immunoblot analysis of ZO-1 protein. Scale bar = 50 um. *p < .05, **p < .01, ***p < .001. All the results were repeated three times. All data were presented as means ± SD (n = 3). TEER, transepithelial electrical resistance measurements.

Application: IHC    Species: mouse    Sample: colon

Figure 5. |FnEVs increase gut barrier leakage in experimental colitis models. (a) Representative images and ex vivo imaging with the intestine, liver, heart, spleen and kidney of mice. (b) Relative fluorescence intensity of translocated EGFP-labeled E.coli in every tissues. (c) Representative images of immunohistochemical stainings of ZO-1, claudin-1 and occludin in the colon on day 3 after colitis induction. Scale bar = 50 um.
9). Versatility of bacterial outer membrane vesicles in regulating intestinal homeostasis. Science Advances, 2023 (PubMed: 36921043) [IF=11.7]
10). Beneficial Effects of Celastrol on Immune Balance by Modulating Gut Microbiota in Experimental Ulcerative Colitis Mice. Genomics, proteomics & bioinformatics, 2022 (PubMed: 35609771) [IF=11.5]
加载更多

限制条款

产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）

产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。

产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。

Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。

产品仅供科学研究使用。不用于诊断和治疗。 

产品未经授权不得转售。

Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.