SIRT3 Antibody - #AF5135

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产品: SIRT3 抗体
货号: AF5135
描述: Rabbit polyclonal antibody to SIRT3
应用: WB IHC IF/ICC
文献验证: WB, IHC, IF/ICC
反应: Human, Mouse, Rat
预测: Sheep, Rabbit
蛋白号: Q9NTG7
RRID: AB_2837621

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
SIRT3 Antibody detects endogenous levels of total SIRT3.
RRID:
AB_2837621
引用格式: Affinity Biosciences Cat# AF5135, RRID:AB_2837621.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

hSIRT 3; hSIRT3; Mitochondrial nicotinamide adenine dinucleotide dependent deacetylase; NAD dependent deacetylase sirtuin 3 mitochondrial; NAD-dependent protein deacetylase sirtuin-3, mitochondrial; Regulatory protein SIR2 homolog 3; Silent mating type information regulation 2 S.cerevisiae homolog 3; Sir 2 like 3; SIR 2 like protein 3; SIR 3; SIR2 L3; Sir2 like 3; SIR2 like protein 3; SIR2-like protein 3; SIR2L3; SIR3_HUMAN; SIRT 3; SIRT3; Sirtuin 3; Sirtuin silent mating type information regulation 2 homolog 3 (S. cerevisiae); Sirtuin type 3; Sirtuin3;

抗原和靶标

免疫原:

A synthesized peptide derived from human SIRT3, corresponding to a region within C-terminal amino acids.

基因/基因ID:
SIRT3(23410)
描述:
NAD-dependent protein deacetylase. Activates mitochondrial target proteins, including ACSS1, IDH2 and GDH by deacetylating key lysine residues. Contributes to the regulation of the cellular energy metabolism. Important for regulating tissue-specific ATP levels.

研究领域

· Human Diseases > Cancers: Overview > Central carbon metabolism in cancer.   (View pathway)

文献引用

1). β-hydroxybutyrylation and O-GlcNAc modifications of STAT1 modulate antiviral defense in aging. Cellular & molecular immunology, 2025 (PubMed: 39979583) [IF=21.8]
2). The deubiquitinase USP11 ameliorates intervertebral disc degeneration by regulating oxidative stress-induced ferroptosis via deubiquitinating and stabilizing Sirt3. Redox biology, 2023 (PubMed: 37099926) [IF=10.7]
3). Sirt3-mediated mitophagy regulates AGEs-induced BMSCs senescence and senile osteoporosis. Redox Biology, 2021 (PubMed: 33662874) [IF=10.7]

Application: WB    Species: mice    Sample: bone marrow mesenchymal stem (BMSCs)

Fig. 2. Effects of different concentrations of AGEs on mitochondrial function and mitophagy of BMSCs. The BMSCs were treated with AGEs (50–200 μg/mL) or BSA for 24–72 h. (A) Representative fluorescence images with DCF (green) staining in BMSCs stimulated with AGEs. Scale bar: 50 μm. (B) Representative fluorescence images with Mito-SOX (red) and Mito-Tracker (green) double-staining in BMSCs stimulated with AGEs. Scale bar: 50 μm. (C) The MMP was detected through JC-1 staining in BMSCs stimulated with AGEs. Scale bar: 50 μm. (D) Representative fluorescence images with Mtphagy Dye (red) and Mito-Tracker (green) double-staining in BMSCs stimulated with AGEs. Scale bar: 50 μm. (E) Representative fluorescence images with LC3B (red) and Mito-Tracker (green) double-staining in BMSCs stimulated with AGEs. Scale bar: 50 μm. (F) Representative Western blotting assay and quantitation of the level of LC3B, P62, Parkin, Sirt3. **p < 0.01 versus BSA. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
4). Vine tea (Ampelopsis grossedentata) ameliorates chronic alcohol-induced hepatic steatosis, oxidative stress, and inflammation via YTHDF2/PGC-1α/SIRT3 axis. FOOD RESEARCH INTERNATIONAL, 2025 [IF=8.0]
5). Vine tea (Ampelopsis grossedentata) ameliorates chronic alcohol-induced hepatic steatosis, oxidative stress, and inflammation via YTHDF2/PGC-1α/SIRT3 axis. Food research international (Ottawa, Ont.), 2025 (PubMed: 40253212) [IF=7.0]
6). Inhibiting mir-34a-5p regulates doxorubicin-induced autophagy disorder and alleviates myocardial pyroptosis by targeting Sirt3-AMPK pathway. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 2023 (PubMed: 37806095) [IF=6.9]

Application: IF/ICC    Species: Mouse    Sample:

Fig. 6. Sirt3 is the direct target of mir-34a-5p. (A) Schematic diagram of binding sites between Mir-34a-5p and Sirt3. (B) The luciferase reporter assays of Mir-34a-5p and Sirt3 (n = 3). (C) Fluorescence of Sirt3 using the inhibitors or mimics of Mir-34a-5p. (D) Quantification the fluorescene of Sirt3 (n = 5). The values are expressed as mean ± SD. NS, not significant, *P 

Application: WB    Species: Rat    Sample: H9C2 cell

Fig. 7. Overexpression of sirt3 alleviates DOX-induced H9C2 cell autophagy and pyroptosis in vitro. (A) Protein level of Sirt3 using SiRNA or plasmid as measured by western blotting and (B) Quantification the expression of Sirt3 (n = 3). (C) Protein level of Sirt3 using siRNA or plasmid as measured by RT-PCR (n = 3). (D) Use SiRNA or Sirt3 plasmid to observe H9C2 cell viability by CCK-8 assay (n = 5). (E) Representative western blots using SiRNA and (F) Quantification the expression of proteins (n = 3). (G) Representative western blots using Sirt3 plasmid and (H) Quantification the expression of proteins (n = 3). (I, J) Detection and quantification of MitoROS (n = 5). (K, L) Detection and quantification of autophagic flux (n = 5). The values are expressed as mean ± SD. NS, not significant, *P 
7). Baicalin Attenuates Diabetic Cardiomyopathy In Vivo and In Vitro by Inhibiting Autophagy and Cell Death Through SENP1/SIRT3 Signaling Pathway Activation. Antioxidants & redox signaling, 2025 (PubMed: 38687336) [IF=5.9]
8). 3-MCPD Induced Mitochondrial Damage of Renal Cells Via the Rhythmic Protein BMAL1 Targeting SIRT3/SOD2. Journal of Agricultural and Food Chemistry, 2023 (PubMed: 37750480) [IF=5.7]
9). PGC-1α activation ameliorates cancer-induced bone pain via inhibiting apoptosis of GABAergic interneurons. Biochemical pharmacology, 2024 (PubMed: 38354958) [IF=5.3]
10). SIRT3-and FAK-mediated acetylation-phosphorylation crosstalk of NFATc1 regulates Nε-carboxymethyl-lysine-induced vascular calcification in diabetes mellitus. Atherosclerosis, 2023 (PubMed: 37392543) [IF=4.9]
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