SIRT3 Antibody - #AF5135

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产品: SIRT3 抗体
货号: AF5135
描述: Rabbit polyclonal antibody to SIRT3
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Sheep, Rabbit
分子量: 29 kDa; 44kD(Calculated).
蛋白号: Q9NTG7
RRID: AB_2837621

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Sheep(88%), Rabbit(88%)
克隆:
Polyclonal
特异性:
SIRT3 Antibody detects endogenous levels of total SIRT3.
RRID:
AB_2837621
引用格式: Affinity Biosciences Cat# AF5135, RRID:AB_2837621.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

hSIRT 3; hSIRT3; Mitochondrial nicotinamide adenine dinucleotide dependent deacetylase; NAD dependent deacetylase sirtuin 3 mitochondrial; NAD-dependent protein deacetylase sirtuin-3, mitochondrial; Regulatory protein SIR2 homolog 3; Silent mating type information regulation 2 S.cerevisiae homolog 3; Sir 2 like 3; SIR 2 like protein 3; SIR 3; SIR2 L3; Sir2 like 3; SIR2 like protein 3; SIR2-like protein 3; SIR2L3; SIR3_HUMAN; SIRT 3; SIRT3; Sirtuin 3; Sirtuin silent mating type information regulation 2 homolog 3 (S. cerevisiae); Sirtuin type 3; Sirtuin3;

抗原和靶标

免疫原:
Uniprot:

Q9NTG7(SIR3_HUMAN) >>Visit HPA database.

基因/基因ID:
SIRT3(23410)
表达:
Q9NTG7 SIR3_HUMAN:

Widely expressed.

描述:
NAD-dependent protein deacetylase. Activates mitochondrial target proteins, including ACSS1, IDH2 and GDH by deacetylating key lysine residues. Contributes to the regulation of the cellular energy metabolism. Important for regulating tissue-specific ATP levels.
序列:
MAFWGWRAAAALRLWGRVVERVEAGGGVGPFQACGCRLVLGGRDDVSAGLRGSHGARGEPLDPARPLQRPPRPEVPRAFRRQPRAAAPSFFFSSIKGGRRSISFSVGASSVVGSGGSSDKGKLSLQDVAELIRARACQRVVVMVGAGISTPSGIPDFRSPGSGLYSNLQQYDLPYPEAIFELPFFFHNPKPFFTLAKELYPGNYKPNVTHYFLRLLHDKGLLLRLYTQNIDGLERVSGIPASKLVEAHGTFASATCTVCQRPFPGEDIRADVMADRVPRCPVCTGVVKPDIVFFGEPLPQRFLLHVVDFPMADLLLILGTSLEVEPFASLTEAVRSSVPRLLINRDLVGPLAWHPRSRDVAQLGDVVHGVESLVELLGWTEEMRDLVQRETGKLDGPDK

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Rabbit
88
Sheep
88
Pig
75
Dog
75
Horse
0
Bovine
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q9NTG7 作为底物

Site PTM Type Enzyme
R51 Methylation
R57 Methylation
S105 Phosphorylation
S110 Phosphorylation
T150 Phosphorylation P06493 (CDK1)
S159 Phosphorylation P06493 (CDK1)
R276 Methylation
T284 Phosphorylation
R356 Methylation
T391 Phosphorylation

研究背景

功能:

NAD-dependent protein deacetylase. Activates or deactivates mitochondrial target proteins by deacetylating key lysine residues. Known targets include ACSS1, IDH, GDH, SOD2, PDHA1, LCAD, SDHA and the ATP synthase subunit ATP5PO. Contributes to the regulation of the cellular energy metabolism. Important for regulating tissue-specific ATP levels. In response to metabolic stress, deacetylates transcription factor FOXO3 and recruits FOXO3 and mitochondrial RNA polymerase POLRMT to mtDNA to promote mtDNA transcription. Acts as a regulator of ceramide metabolism by mediating deacetylation of ceramide synthases CERS1, CERS2 and CERS6, thereby increasing their activity and promoting mitochondrial ceramide accumulation (By similarity).

翻译修饰:

Processed by mitochondrial processing peptidase (MPP) to give a 28 kDa product. Such processing is probably essential for its enzymatic activity.

细胞定位:

Mitochondrion matrix.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Widely expressed.

亚基结构:

Upon metabolic stress, forms a complex composed of FOXO3, SIRT3 and mitochondrial RNA polymerase POLRMT; the complex is recruited to mtDNA in a SIRT3-dependent manner. Also forms a complex composed of FOXO3, SIRT3, TFAM and POLRMT. Interacts with NDUFA9, ACSS1, IDH2 and GDH. Interacts with PCCA.

蛋白家族:

Belongs to the sirtuin family. Class I subfamily.

研究领域

· Human Diseases > Cancers: Overview > Central carbon metabolism in cancer.   (View pathway)

文献引用

1). Sirt3-mediated mitophagy regulates AGEs-induced BMSCs senescence and senile osteoporosis. Redox Biology (PubMed: 33662874) [IF=11.4]

Application: WB    Species: mice    Sample: bone marrow mesenchymal stem (BMSCs)

Fig. 2. Effects of different concentrations of AGEs on mitochondrial function and mitophagy of BMSCs. The BMSCs were treated with AGEs (50–200 μg/mL) or BSA for 24–72 h. (A) Representative fluorescence images with DCF (green) staining in BMSCs stimulated with AGEs. Scale bar: 50 μm. (B) Representative fluorescence images with Mito-SOX (red) and Mito-Tracker (green) double-staining in BMSCs stimulated with AGEs. Scale bar: 50 μm. (C) The MMP was detected through JC-1 staining in BMSCs stimulated with AGEs. Scale bar: 50 μm. (D) Representative fluorescence images with Mtphagy Dye (red) and Mito-Tracker (green) double-staining in BMSCs stimulated with AGEs. Scale bar: 50 μm. (E) Representative fluorescence images with LC3B (red) and Mito-Tracker (green) double-staining in BMSCs stimulated with AGEs. Scale bar: 50 μm. (F) Representative Western blotting assay and quantitation of the level of LC3B, P62, Parkin, Sirt3. **p < 0.01 versus BSA. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
2). 3-MCPD Induced Mitochondrial Damage of Renal Cells Via the Rhythmic Protein BMAL1 Targeting SIRT3/SOD2. Journal of Agricultural and Food Chemistry (PubMed: 37750480) [IF=6.1]
3). Promising anti-ovarian aging herbal formulation He's Yangchao promotes in vitro maturation of oocytes from advanced maternal age mice. Journal of Ethnopharmacology (PubMed: 37423514) [IF=5.4]
4). SIRT3-and FAK-mediated acetylation-phosphorylation crosstalk of NFATc1 regulates Nε-carboxymethyl-lysine-induced vascular calcification in diabetes mellitus. Atherosclerosis (PubMed: 37392543) [IF=5.3]
5). Single-walled carbon nanohorn aggregates promotes mitochondrial dysfunction-induced apoptosis in hepatoblastoma cells by targeting SIRT3. INTERNATIONAL JOURNAL OF ONCOLOGY (PubMed: 29956732) [IF=5.2]

Application: WB    Species: mouse    Sample: HepG2 cell

Figure 3.| Treatment with SWNHs alters the expression of mitochondrial apoptotic pathway-associated proteins in vitro. (A) HepG2 and (B) L02 cells were incubated with different concentrations of SWNHs in 6-well plates for 48 h. The changes in the expression and the relative quantification of proteins in (C) HepG2 cells or (D) L02 cells were identified by western blotting. SW0, SW10, SW20, SW30, SW40 and SW50 correspond to the concentrations of SWNHs: 0, 0.21, 0.42, 0.64, 0.85 and 0.96 µg/cm2, respectively. Data are presented as the mean ± standard deviation (n=3). *P<0.05 compared with the SW0 group. AceCS2, acyl-CoA synthetase short chain family member 1; SCNN1A, sodium channel epithelial 1α subunit; SIRT3, sirtuin 3; SWNH, single-walled carbon nanohorn; VDAC1, voltage-dependent anion channel 1.
6). miR-494 induces EndMT and promotes the development of HCC (Hepatocellular Carcinoma) by targeting SIRT3/TGF-β/SMAD signaling pathway. Scientific Reports (PubMed: 31076630) [IF=4.6]

Application: IHC    Species: human    Sample: non-tumorous, para-tumorous, and tumorous tissues

Figure 5.|SIRT3 expression in non-tumorous, para-tumorous, and tumorous tissues. Te immunohistochemistry results of liver tissues showed that SIRT3 was positive in all cases. However, the positive intensity and express location in diferent groups were diferences. As showed in this Figure, SIRT3 mainly appeared in the cytoplasm in tumor tissue, but they were at the same time appearing in the cytoplasm and nucleus of para-tumorous and non-tumorous tissues. Moreover, the positive intensity of tumor tissue much lower than para-tumorous or non-tumorous tissues. Te diferences of SIRT3 positive intensity between tumorous and para-tumorous or non-tumorous tissue were statistically signifcant. Te data are presented as means ± SD from three independent experiments. ***P < 0.001.
7). O-GlcNAcylation orchestrates porcine oocyte maturation through maintaining mitochondrial dynamics and function. Molecular human reproduction (PubMed: 38265252) [IF=4.0]
8). Titanium dioxide nanoparticles induce mitochondria-associated apoptosis in HepG2 cells. RSC Advances (PubMed: 35548213) [IF=3.9]
9). Sirtuin (Sirt) 3 Overexpression Prevents Retinopathy in Streptozotocin-Induced Diabetic Rats. MEDICAL SCIENCE MONITOR (PubMed: 32506069) [IF=3.1]

Application: WB    Species: rat    Sample: retinal

Figure 1. |Adenoviral-encoding Sirt3 increased Sirt3 expression in retinal tissue.(E) Protein expression of Sirt3 detected by western blotting; (versus the control group, * P<0.05; versus the model group, # P<0.05). Sirt3 – sirtuin; PCR – polymerase chain reaction; mRNA – messenger RNA.
10). Aerobic Exercise Attenuates Myocardial Ischemia-Reperfusion Injury in Rats by Regulating The SIRT3/SOD2/NF-κB Pathway.

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