产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# BF0290, RRID:AB_2833702.
展开/折叠
Adhesion related kinase; AI323647; Ark; Axl; AXL oncogene; AXL receptor tyrosine kinase; AXL transforming gene; AXL transforming sequence/gene; EC 2.7.10.1; JTK11; Oncogene AXL; Tyro7; Tyrosine protein kinase receptor UFO; Tyrosine-protein kinase receptor UFO; UFO; UFO_HUMAN;
抗原和靶标
Purified recombinant fragment of human AXL expressed in E. Coli.
Highly expressed in metastatic colon tumors. Expressed in primary colon tumors. Weakly expressed in normal colon tissue.
- P30530 UFO_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAWRCPRMGRVPLAWCLALCGWACMAPRGTQAEESPFVGNPGNITGARGLTGTLRCQLQVQGEPPEVHWLRDGQILELADSTQTQVPLGEDEQDDWIVVSQLRITSLQLSDTGQYQCLVFLGHQTFVSQPGYVGLEGLPYFLEEPEDRTVAANTPFNLSCQAQGPPEPVDLLWLQDAVPLATAPGHGPQRSLHVPGLNKTSSFSCEAHNAKGVTTSRTATITVLPQQPRNLHLVSRQPTELEVAWTPGLSGIYPLTHCTLQAVLSDDGMGIQAGEPDPPEEPLTSQASVPPHQLRLGSLHPHTPYHIRVACTSSQGPSSWTHWLPVETPEGVPLGPPENISATRNGSQAFVHWQEPRAPLQGTLLGYRLAYQGQDTPEVLMDIGLRQEVTLELQGDGSVSNLTVCVAAYTAAGDGPWSLPVPLEAWRPGQAQPVHQLVKEPSTPAFSWPWWYVLLGAVVAAACVLILALFLVHRRKKETRYGEVFEPTVERGELVVRYRVRKSYSRRTTEATLNSLGISEELKEKLRDVMVDRHKVALGKTLGEGEFGAVMEGQLNQDDSILKVAVKTMKIAICTRSELEDFLSEAVCMKEFDHPNVMRLIGVCFQGSERESFPAPVVILPFMKHGDLHSFLLYSRLGDQPVYLPTQMLVKFMADIASGMEYLSTKRFIHRDLAARNCMLNENMSVCVADFGLSKKIYNGDYYRQGRIAKMPVKWIAIESLADRVYTSKSDVWSFGVTMWEIATRGQTPYPGVENSEIYDYLRQGNRLKQPADCLDGLYALMSRCWELNPQDRPSFTELREDLENTLKALPPAQEPDEILYVNMDEGGGYPEPPGAAGGADPPTQPDPKDSCSCLTAAEVHPAGRYVLCPSTTPSPAQPADRGSPAAPGQEDGA
翻译修饰 - P30530 作为底物
Site | PTM Type | Enzyme | Source |
---|---|---|---|
S100 | Phosphorylation | Uniprot | |
T200 | Phosphorylation | Uniprot | |
S201 | Phosphorylation | Uniprot | |
S202 | Phosphorylation | Uniprot | |
S204 | Phosphorylation | Uniprot | |
T303 | Phosphorylation | Uniprot | |
Y481 | Phosphorylation | Uniprot | |
S503 | Phosphorylation | Uniprot | |
S515 | Phosphorylation | Uniprot | |
K523 | Ubiquitination | Uniprot | |
K525 | Ubiquitination | Uniprot | |
K535 | Ubiquitination | Uniprot | |
K540 | Ubiquitination | Uniprot | |
K563 | Ubiquitination | Uniprot | |
K570 | Ubiquitination | Uniprot | |
S612 | Phosphorylation | Uniprot | |
Y634 | Phosphorylation | Uniprot | |
Y643 | Phosphorylation | Uniprot | |
K666 | Ubiquitination | Uniprot | |
S694 | Phosphorylation | Uniprot | |
K696 | Ubiquitination | Uniprot | |
Y698 | Phosphorylation | Uniprot | |
Y702 | Phosphorylation | Uniprot | |
Y703 | Phosphorylation | Uniprot | |
K710 | Ubiquitination | Uniprot | |
K714 | Ubiquitination | Uniprot | |
R724 | Methylation | Uniprot | |
Y726 | Phosphorylation | Uniprot | |
Y759 | Phosphorylation | Uniprot | |
Y772 | Phosphorylation | Uniprot | |
Y779 | Phosphorylation | P30530 (AXL) , P00533 (EGFR) | Uniprot |
Y814 | Phosphorylation | Uniprot | |
Y821 | Phosphorylation | P30530 (AXL) | Uniprot |
S853 | Phosphorylation | Uniprot | |
Y866 | Phosphorylation | P30530 (AXL) | Uniprot |
T873 | Phosphorylation | Uniprot | |
S875 | Phosphorylation | Uniprot | |
S884 | Phosphorylation | Uniprot |
翻译修饰 - P30530 作为激酶
研究背景
Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding growth factor GAS6 and which is thus regulating many physiological processes including cell survival, cell proliferation, migration and differentiation. Ligand binding at the cell surface induces dimerization and autophosphorylation of AXL. Following activation by ligand, ALX binds and induces tyrosine phosphorylation of PI3-kinase subunits PIK3R1, PIK3R2 and PIK3R3; but also GRB2, PLCG1, LCK and PTPN11. Other downstream substrate candidates for AXL are CBL, NCK2, SOCS1 and TNS2. Recruitment of GRB2 and phosphatidylinositol 3 kinase regulatory subunits by AXL leads to the downstream activation of the AKT kinase. GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response.
(Microbial infection) Acts as a receptor for lassa virus and lymphocytic choriomeningitis virus, possibly through GAS6 binding to phosphatidyl-serine at the surface of virion envelope.
(Microbial infection) Acts as a receptor for Ebolavirus, possibly through GAS6 binding to phosphatidyl-serine at the surface of virion envelope.
Monoubiquitinated upon GAS6-binding. A very small proportion of the receptor could be subjected to polyubiquitination in a very transient fashion.
Phosphorylated at tyrosine residues by autocatalysis, which activates kinase activity.
Cell membrane>Single-pass type I membrane protein.
Highly expressed in metastatic colon tumors. Expressed in primary colon tumors. Weakly expressed in normal colon tissue.
Heterodimer and heterotetramer with ligand GAS6. Interacts with CBL, GRB2, LCK, NCK2, PIK3R1, PIK3R2, PIK3R3, PLCG1, SOCS1 and TNS2. Part of a complex including AXL, TNK2 and GRB2, in which GRB2 promotes AXL recruitment by TNK2.
Belongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily.
研究领域
· Human Diseases > Drug resistance: Antineoplastic > EGFR tyrosine kinase inhibitor resistance.
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