Smad9 Antibody - #AF5114

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产品: Smad9 抗体
货号: AF5114
描述: Rabbit polyclonal antibody to Smad9
应用: WB IHC
文献验证: WB
反应: Human, Mouse, Rat
预测: Sheep, Chicken
蛋白号: O15198
RRID: AB_2837600

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
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实验方案
WB Handbook
IHC Protocol
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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Smad9 Antibody detects endogenous levels of total Smad9.
RRID:
AB_2837600
引用格式: Affinity Biosciences Cat# AF5114, RRID:AB_2837600.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

MAD homolog 9; Madh6; Mothers against decapentaplegic; Mothers against decapentaplegic homolog 9; Mothers against DPP homolog 9; SMAD 9; SMAD family member 9; SMAD, mothers against DPP homolog 9 (Drosophila); SMAD8A; SMAD8B; Smad9; SMAD9_HUMAN;

抗原和靶标

免疫原:

A synthesized peptide derived from human Smad9, corresponding to a region within the internal amino acids.

基因/基因ID:
SMAD9(4093)
描述:
SMAD9 is a member of the MAD-related family of molecules. MAD-related proteins are a recently identified family of intracellular proteins that are thought to be essential components in the signaling pathways of the serine/threonine kinase receptors of the transforming growth factor beta superfamily.

研究领域

· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells.   (View pathway)

· Environmental Information Processing > Signal transduction > TGF-beta signaling pathway.   (View pathway)

文献引用

1). Dual-function MgSrCeAl-LDH nanosheets enable both ROS clearance and enhanced angiogenesis for myocardial ischemia/reperfusion injury therapy. Journal of nanobiotechnology, 2025 (PubMed: 41139778) [IF=10.2]

Application: WB    Species: Rat    Sample:

Fig. 6 (a) Cluster analysis of total differentially expressed genes among the Control, OGD/R, and OGD/R + LDH groups, P 0.58, n = 3. (b) Volcano plot analysis of total differentially expressed genes between Control and OGD/R groups, P 0.58. (c) Volcano plot analysis of total differentially expressed genes between OGD/R and OGD/R + LDH groups, P 0.58, n = 3. (d) KEGG enrichment analysis of total differentially expressed genes between Control and OGD/R groups, P 1, n = 3. (e) KEGG enrichment analysis of total differentially expressed genes between OGD/R and OGD/R + LDH groups, P 1, n = 3. (f) Representative western blot bands of p-PI3K/PI3K/p-AKT/AKT/TGF-β/Smad9/β-actin after different treatments. (g) Quantification of p-PI3K/PI3K and p-AKT/AKT protein levels from (f), n = 4. (h) Co-staining images of p-PI3K9 (green) and α-actinin (red) after different treatments on Day 14, Scale bar = 20 μm, n = 5. (i, j) Heat map of expressed genes related to apoptosis signaling pathway and sprouting angiogenesis among the Control, OGD/R and OGD/R + LDH groups, P 0.58, n = 3. (k) GSEA analysis of sprouting angiogenesis between OGD/R and OGD/R + LDH groups, P 1.0, n = 3. (l) GSEA analysis of TGF-β signaling pathway between OGD/R and OGD/R + LDH groups, P 1.0, n = 3. (m) Heat map of expressed genes related to TGF-β signaling pathway among the Control, OGD/R and OGD/R + LDH groups, P 0.58, n = 3. (n) Quantification of TGF-β2 and Smad9 protein levels from (f), n = 4. Data are presented as mean values ± SEM

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