OPRM1 Antibody - #DF5045

Fig. 5Disruption of the affect protamine replacement in Cdkn2aip-/- mice. A Immunofluorescence staining of histone H3 in seminiferous tubules of Cdkn2aip+/+ and Cdkn2aip−/− testes. histone H3(green) and DAPI (blue). Scale bar, 50 μm. B Immunofluorescence staining of TNP1 in seminiferous tubules of Cdkn2aip+/+ and Cdkn2aip−/− testes. TNP1 (green) and DAPI (blue). Scale bar, 50 μm. C and D Immunofluorescence staining of PRM1 and PRM2 in seminiferous tubules of Cdkn2aip+/+ and Cdkn2aip−/− testes. PRMs(green) and DAPI (blue). Scale bar, 50 μm. E qPCR analyses showing significantly reduced levels of Prm1 and Prm2 in 8-week-old Cdkn2aip+/+ and Cdkn2aip−/− testis. Data are presented as Data are presented as mean ± S.D. Student’s t test; *P < 0.05, ***P < 0.001. F Quantification of relative protein level of PRM1 and PRM2 by Western blotting in Cdkn2aip+/+ and Cdkn2aip−/− testis. GAPDH is serves as a loading control

Fig. 5Disruption of the affect protamine replacement in Cdkn2aip-/- mice. A Immunofluorescence staining of histone H3 in seminiferous tubules of Cdkn2aip+/+ and Cdkn2aip−/− testes. histone H3(green) and DAPI (blue). Scale bar, 50 μm. B Immunofluorescence staining of TNP1 in seminiferous tubules of Cdkn2aip+/+ and Cdkn2aip−/− testes. TNP1 (green) and DAPI (blue). Scale bar, 50 μm. C and D Immunofluorescence staining of PRM1 and PRM2 in seminiferous tubules of Cdkn2aip+/+ and Cdkn2aip−/− testes. PRMs(green) and DAPI (blue). Scale bar, 50 μm. E qPCR analyses showing significantly reduced levels of Prm1 and Prm2 in 8-week-old Cdkn2aip+/+ and Cdkn2aip−/− testis. Data are presented as Data are presented as mean ± S.D. Student’s t test; *P < 0.05, ***P < 0.001. F Quantification of relative protein level of PRM1 and PRM2 by Western blotting in Cdkn2aip+/+ and Cdkn2aip−/− testis. GAPDH is serves as a loading control

Figure 1 The expression of KOR and MOR in HCC cell lines and human HCC tissues. (A) The expression of KOR and MOR were detected in LO2, Hep3B and Huh7 cells by immunofluorescent staining, scale bar, 25um. The expression of KOR and MOR was evaluated in 4 types of HCC cell lines (HepG2, Bel-7402, Hep3B and Huh-7) by RT-qPCR (B) and western blotting (C). (D) In human HCC tissues, KOR and MOR were detected by western blotting, GADPH was used as an internal control. N, Non-tumor; T, Tumor. Values are presented as the mean ± standard deviation (n = 3). KOR, kappa opioid receptor; MOR, mu opioid receptor; GADPH, glyceraldehyde-3-phosphate dehydrogenase.

Figure 1 The expression of KOR and MOR in HCC cell lines and human HCC tissues. (A) The expression of KOR and MOR were detected in LO2, Hep3B and Huh7 cells by immunofluorescent staining, scale bar, 25um. The expression of KOR and MOR was evaluated in 4 types of HCC cell lines (HepG2, Bel-7402, Hep3B and Huh-7) by RT-qPCR (B) and western blotting (C). (D) In human HCC tissues, KOR and MOR were detected by western blotting, GADPH was used as an internal control. N, Non-tumor; T, Tumor. Values are presented as the mean ± standard deviation (n = 3). KOR, kappa opioid receptor; MOR, mu opioid receptor; GADPH, glyceraldehyde-3-phosphate dehydrogenase.

Figure 5. Changes of spinal MOR and KOR protein expression over time in tumor-implanted mice and sham-implanted mice. Western blot for β-actin, MOR, and KOR resulted in products of 42, 45, and 43 kDa, as expected (markers show predicted band sizes) (a and b). Densitometric quantification of β-actin, MOR, and KOR immunoreactivity on Western blots (c and d). Data were presented as fold change of control (Naive) mean ± SD.*p < 0.05 vs. sham-implanted mice. (n = 4 per group). (Interaction: F-statistics = 15.92/17.71; Row Factor: F-statistics = 43.83/25.90; Column Factor: F-statistics = 115.70/113.90, MOR protein/KOR protein respectively).