GPR132 Antibody - #DF4894

Fig. 1. The expression of GPR132 decreased in activated NK cells. (A to C) RNA-seq analysis of gene expression in activated NK cells compared with a control group (n = 3). (A) Heatmap shows the expression variation of NK activation–related markers and inhibition of NK activation markers. (B) Volcano plot shows 3446 genes shared by three donors. Down-regulated genes, blue; up-regulated genes, yellow; interested genes, red. (C) Column chart shows the expression of the interested GPCR gene according to FPKM value. (D) RT-PCR to detect the GPR132 expression in NK92 cells after being stimulated by ionomycin (1 μg/ml) and PMA (20 ng/ml) at 0, 6, or 12 hours. β-Actin was used as a reference housekeeping gene (n = 3, biological replicates). (E) WB determined the GPR132 expression in NK92 cells after being stimulated by ionomycin (1 μg/ml) and PMA (20 ng/ml) for 12 hours. β-Tubulin was used as a reference housekeeping gene. Data represent means ± SD. Analyzed by two-way analysis of variance (ANOVA), one-way ANOVA, or unpaired t test (*P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001).