OPN3 Antibody - #DF4877

Fig. 3: UVA induces keratinocyte supranuclear melanin cap via OPN3.a OPN3 expression (green) colocalized with HEK marker Pan-CK (red) in skin explant with immunofluorescence staining, without UVA (top) or with UVA irradiation (bottom). Nuclei were counterstained with DAPI. Masson-Fontana (MF) staining demonstrated melanin cap formation. Images were analyzed by brightfield/fluorescence microscopy. Scale bar = 20 µm. b After siRNA inhibited OPN3 irradiated without or with UVA, WB was used to analyze changes in OPN3, Dync1i1, and DCTN1 expression levels in HEK. WB analyses were normalized using β-tubulin as a loading control, and the relative protein level was quantified using Quantity One software. n = 3 independent experiments. Statistical significance was determined by one-ANOVA with post-test. **P 

Fig. 2: UVA increases OPN3 expression in human epidermal keratinocytes.a, b RT-qPCR analysis of OPNs gene expression levels in HaCaT and HEK. OPNs mRNA levels were normalized to GAPDH levels (n = 3 independent experiments). Statistical significance was determined by one-ANOVA with post-test. ****P 

Fig. 6. Piezo1 on PANoptosis triggered by bone infection. (a) Infection human clinical tissues and healthy human clinical tissues: PIEZO1 and PANoptosis related markers (CASP1, CASPA3, NLRP3) were assessed through immunohistochemical staining. (b) Infected bone marrow cavity tissues and healthy tissues of rats: PIEZO1 and PANoptosis related markers (GSDMD, CASPA3, RIPK3) were assessed through immunohistochemical staining. (c) Semi-quantitative analysis of PIEZO1 and PANoptosis related markers (GSDME, CASPASE3, RIPK3) in the human clinical tissues. (d) Semi-quantitative analysis of PIEZO1 and PANoptosis related markers (GSDME, CASPASE3, RIPK3) in the bone marrow cavity tissue of rats. (e) Western blot of PANoptosis related markers (GSDMD, CASP1, CASP3, CASP8, pRIPK3, pMLKL). n = 3 independent experiments per group, *P < 0. 05; **P < 0. 01.