AGTRL1 Antibody - #DF4853

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产品: AGTRL1 抗体
货号: DF4853
描述: Rabbit polyclonal antibody to AGTRL1
应用: WB IHC IF/ICC
文献验证: WB
反应: Human, Mouse, Rat
预测: Bovine, Horse, Rabbit, Dog
蛋白号: P35414
RRID: AB_2837217

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
AGTRL1 Antibody detects endogenous levels of total AGTRL1.
RRID:
AB_2837217
引用格式: Affinity Biosciences Cat# DF4853, RRID:AB_2837217.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

AGTR L1; AGTRL 1; AGTRL1; Angiotensin II receptor like 1; Angiotensin receptor like 1; Angiotensin receptor-like 1; Apelin receptor; APJ (apelin) receptor; APJ; APJ receptor; APJ_HUMAN; APJR; APLNR; B78; FLJ90771; G protein coupled receptor APJ; G-protein coupled receptor APJ; G-protein coupled receptor HG11; GPCR34; HG 11; HG11; HG11 orphan receptor; MGC45246; MSR; msr/apj;

抗原和靶标

免疫原:

A synthesized peptide derived from human AGTRL1, corresponding to a region within the internal amino acids.

基因/基因ID:
APLNR(187)

研究领域

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Environmental Information Processing > Signal transduction > Apelin signaling pathway.   (View pathway)

文献引用

1). The extract of an herbal medicine Chebulae fructus inhibits hepatocellular carcinoma by suppressing the Apelin/APJ system. Frontiers in pharmacology, 2024 (PubMed: 38881868) [IF=5.6]

Application: WB    Species: Human    Sample: HCC cells

CFE inhibits the Apelin/APJ in HCC cells. (A) The transcriptome analysis was performed between ctrl- or CFE-treated PLC/PRF/5 cells. PCA analysis of ctrl and CFE groups. (B) The volcano diagram of ctrl and CFE groups. (C) The histogram of KEGG pathways. (D) Protein-protein interaction network of the differential genes related to signal transduction and cancer: overview. The dot size and number of connections indicate the importance of the protein in the network. (E) The heatmap of the top 10 important genes in the protein-protein interaction network. QRT-PCR assays were performed to detect the expression of (F) GNGT1, ADCY10, PIK3R2, (G) Aplein, and (H) APJ in PLC/PRF/5 cells after treated with or without CFE. (I) The level of Apelin in the serum of mice treated with vehicle control, CFE, or 5-FU using ELISA assays. (J) IHC staining of APJ in tumor tissues from different treatment groups. Scale bar: 50 μm. (K) Protein levels of APJ in PLC/PRF/5 and HepG2 cells are determined using Western blotting. GAPDH serves as a loading control. Representative bands are shown in the left panel, and relative band intensities are shown in the right panel. The data were presented as mean ± SD. *p < 0.05, **p < 0.01.

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