Ferritin Heavy Chain Antibody - #DF4828

FIGURE 4 Panax notoginseng saponins (PNS) suppresses ferroptosis by regulating iron metabolism and reducing lipid peroxidation. (a) Analysis of high-fat diet (HFD)-induced iron deposition in the aortic root of mice. The aortic root sections were stained with Prussian Blue and the iron content was quantified, n = 10. (b) Immunofluorescence analysis of GPX4 in aortic root sections from apoE−/− mice, and mean fluorescence intensity was quantified, n = 10. (c–e) Serum MDA, SOD and CAT levels of apoE−/− mice were measured by Elisa assay kits, n = 10. (f) Quantification of expression of genes encoding for lipid peroxide clearance (Gpx4 and Slc7A11), peroxide generation (Acsl4 and Lpcat3) and iron metabolism (Fth), with qRT-PCR, in peritoneal macrophages from apoE−/− mice, n = 6. (g) Quantification of NCOA4, SLC7A11, FtH and GPX4 protein expression via western blotting in peritoneal macrophages from apoE−/− mice, n = 6. Data shown are means ± SEM (n = 6). *P < 0.05, significantly different from control (Ctrl).