KLRC1 Antibody - #DF4808

Figure 6. Pathogenic role of Treg cytotoxic transition to the orbital lesions of GO. (A) Schematic diagram of the EGFP labeled autoreactive Treg adoptive transfer experiment. (B) The proportion of EGFP+KLRC1+ cells within CD4+ cells in the peripheral blood of Healthy and GO mice after adoptive transfer experiment, representing the cytotoxic transition of Treg in vivo (Healthy, n = 3; GO, n = 9). Data are represented as the median IQR. *P < 0.05 by Mann-Whitney U test. (C) Immunofluorescence staining demonstrated the presence of KLRC1+EGFP+ cells in the orbital tissues of GO model mice. (D and E) The major subtypes of orbital tissues (D) and integrated CD4 T cells from PBMCs and tissues (E) based on scRNA-Seq data of Healthy donors and patients with GO; the upper right UMAP plot illustrates the batch effects. (F) The ligand-receptor interactions involving CD4 CTLs significantly altered in GO among 3 LPF subgroups, MYF, and COF. lipofibroblast, LPF; myofibroblast, MYF; conventional orbital fibroblast, COF. Red represents inflammation-regulating ligand-receptor interactions, while green indicates ligand-receptor interactions related to extracellular matrix remodeling. (G) Experimental model of the pathogenic effect of Treg cytotoxic transformation on localized lesions in the orbit. (H) Immunofluorescence showed the expression of specific proteins of OF cells in the coculture system of healthy control and GO model mouse groups; the histograms represent the quantitative statistics of these proteins. Data are represented as the median IQR. **P < 0.001 by Mann-Whitney U test. (I) qPCR histogram showed the expression of specific genes in cells of the coculture system. Each group consists of 3 independent samples (each n = 6). Data are represented as the median IQR. **P < 0.001 by Mann-Whitney U test. All experiments were repeated 3 times.

FIGURE 4 The mRNA and protein expression level of CCL13 and KLRC1 in UCEC tumor and the adjacent tissues. (A,B) The mRNA expression of CCL13 and KLRC1 in UCEC tumor and the adjacent tissues. (C,D) The protein expression of CCL13 and KLRC1 in UCEC tumor and the adjacent tissues observed using IHC.

FIGURE 4 The mRNA and protein expression level of CCL13 and KLRC1 in UCEC tumor and the adjacent tissues. (A,B) The mRNA expression of CCL13 and KLRC1 in UCEC tumor and the adjacent tissues. (C,D) The protein expression of CCL13 and KLRC1 in UCEC tumor and the adjacent tissues observed using IHC.

FIGURE 4 The mRNA and protein expression level of CCL13 and KLRC1 in UCEC tumor and the adjacent tissues. (A,B) The mRNA expression of CCL13 and KLRC1 in UCEC tumor and the adjacent tissues. (C,D) The protein expression of CCL13 and KLRC1 in UCEC tumor and the adjacent tissues observed using IHC.