Cytochrome P450 8B1 Antibody - #DF4762

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产品: Cytochrome P450 8B1 抗体
货号: DF4762
描述: Rabbit polyclonal antibody to Cytochrome P450 8B1
应用: WB IHC
反应: Human, Mouse
预测: Pig, Horse, Sheep, Rabbit, Dog
分子量: 58 KD; 58kD(Calculated).
蛋白号: Q9UNU6
RRID: AB_2837116

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 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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MS Report
HPLC Report
MSDS
说明书
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse
预测:
Pig(92%), Horse(%), Sheep(%), Rabbit(%), Dog(%)
克隆:
Polyclonal
特异性:
Cytochrome P450 8B1 Antibody detects endogenous levels of total Cytochrome P450 8B1.
RRID:
AB_2837116
引用格式: Affinity Biosciences Cat# DF4762, RRID:AB_2837116.
偶联:
Unconjugated. 130
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

7-alpha-hydroxy-4-cholesten-3-one 12-alpha-hydroxylase; 7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase; CP8B; CP8B1_HUMAN; CYP12; CYP8B1; CYPVIIIB1; Cytochrome P450 8B1; Cytochrome P450 subfamily VIIIB (sterol 12 alpha hydroxylase) polypeptide 1; Cytochrome P450, family 8, subfamily B, polypeptide 1; Sterol 12-alpha-hydroxylase;

抗原和靶标

免疫原:
Uniprot:

Q9UNU6(CP8B1_HUMAN) >>Visit HPA database.

基因/基因ID:
CYP8B1(1582)
表达:
Q9UNU6 CP8B1_HUMAN:

Liver.

序列:
MVLWGPVLGALLVVIAGYLCLPGMLRQRRPWEPPLDKGTVPWLGHAMAFRKNMFEFLKRMRTKHGDVFTVQLGGQYFTFVMDPLSFGSILKDTQRKLDFGQYAKKLVLKVFGYRSVQGDHEMIHSASTKHLRGDGLKDLNETMLDSLSFVMLTSKGWSLDASCWHEDSLFRFCYYILFTAGYLSLFGYTKDKEQDLLQAGELFMEFRKFDLLFPRFVYSLLWPREWLEVGRLQRLFHKMLSVSHSQEKEGISNWLGNMLQFLREQGVPSAMQDKFNFMMLWASQGNTGPTSFWALLYLLKHPEAIRAVREEATQVLGEARLETKQSFAFKLGALQHTPVLDSVVEETLRLRAAPTLLRLVHEDYTLKMSSGQEYLFRHGDILALFPYLSVHMDPDIHPEPTVFKYDRFLNPNGSRKVDFFKTGKKIHHYTMPWGSGVSICPGRFFALSEVKLFILLMVTHFDLELVDPDTPLPHVDPQRWGFGTMQPSHDVRFRYRLHPTE

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
92
Sheep
92
Dog
92
Rabbit
92
Horse
85
Chicken
69
Zebrafish
54
Bovine
0
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

功能:

Involved in bile acid synthesis and is responsible for the conversion of 7 alpha-hydroxy-4-cholesten-3-one into 7 alpha, 12 alpha-dihydroxy-4-cholesten-3-one. Responsible for the balance between formation of cholic acid and chenodeoxycholic acid. Has a rather broad substrate specificity including a number of 7-alpha-hydroxylated C27 steroids.

细胞定位:

Endoplasmic reticulum membrane>Single-pass membrane protein. Microsome membrane>Single-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Liver.

蛋白家族:

Belongs to the cytochrome P450 family.

研究领域

· Metabolism > Lipid metabolism > Primary bile acid biosynthesis.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Endocrine system > PPAR signaling pathway.

文献引用

1). Geniposidic Acid Targeting FXR "S332 and H447" Mediated Conformational Change to Upregulate CYPs and miR-19a-3p to Ameliorate Drug-Induced Liver Injury. Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2025 (PubMed: 39998442) [IF=15.1]
2). Geniposidic Acid Targeting FXR "S332 and H447" Mediated Conformational Change to Upregulate CYPs and miR-19a-3p to Ameliorate Drug-Induced Liver Injury. Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2025 (PubMed: 39998442) [IF=14.3]
3). Obeticholic acid protects against lithocholic acid-induced exogenous cell apoptosis during cholestatic liver injury. Life sciences, 2024 (PubMed: 38104861) [IF=5.2]
4). Antarctic krill peptide alleviated liver fibrosis via downregulating secondary bile acid activated NLRP3 signaling pathway. Food & Function, 2022 (PubMed: 35762853) [IF=5.1]
5). Sanye Tablet regulates gut microbiota and bile acid metabolism to attenuate hepatic steatosis. Journal of ethnopharmacology, 2025 (PubMed: 39971018) [IF=4.8]
6). An integrated strategy combining network toxicology and feature-based molecular networking for exploring hepatotoxic constituents and mechanism of Epimedii Folium-induced hepatotoxicity in vitro. Food and Chemical Toxicology, 2023 (PubMed: 37080529) [IF=3.9]

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