Cytochrome P450 8B1 Antibody - #DF4762

Figure 4 GPA promotes cholesterol metabolism by accelerating the synthesis and efflux of primary bile acids. A) Dynamic distribution map of differences in lipid content. B) Differential lipid KEGG classification map. C) Differential lipid KEGG enrichment map. D) Primary free bile acid content in mouse liver (n = 6). E) Mouse liver primary conjugated bile acid content (n = 6). F) Mouse liver secondary conjugated bile acid content (n = 6). G) Secondary free bile acid content in mouse liver (n = 6). H,I) The mRNA and protein expression of hepatic BSEP, CYP7A1, CYP8B1, CYP27A1, and CYP7B1 in TP chronic DILI mice treated with GPA (n = 6). All mRNA levels were measured by qPCR, normalized to GAPDH. J,K) The mRNA and protein expression of hepatic BSEP, CYP7A1, CYP8B1, CYP27A1, and CYP7B1 in APAP acute DILI mice treated with GPA (n = 6). All mRNA levels were measured by qPCR, normalized to GAPDH. L,M) The mRNA and protein expression of hepatic BSEP, CYP7A1, CYP8B1, CYP27A1, and CYP7B1 in TP chronic DILI mice treated with GPA (n = 6). All mRNA levels were measured by qPCR, normalized to GAPDH. N) Summary of upregulating CYPs-transporter-mediated primary bile acid synthesis and transport of GPA treatment of acute and chronic DILI. All data are presented as means ± SD. Compared with control group, *P < 0.05, **P < 0.01, ***P < 0.001. Compared with model group, #P < 0.05, ##P < 0.01, ###P < 0.001. All data are via one-way analysis of variance (ANOVA).

Figure 4. Specnuezhenide (SPN) upregulates the protein expression of bile acid (BA) enzymes in D−galactose (D−gal) mice. (A) Immunohistochemistry (IHC) analysis of cytochrome P4507A1 (CYP7A1), cytochrome P45027A1 (CYP27A1), cytochrome P4507B1 (CYP7B1), and cytochrome P4508B1 (CYP8B1) in the liver (black arrows, n = 5). (B) The levels of CYP7A1, CYP27A1, CYP7B1, and CYP8B1 were analyzed using western blotting. The densitometric quantification of CYP7A1, CYP27A1, CYP7B1, and CYP8B1 is shown in the right panels (n = 3). Data are expressed as mean ± SD. ** p < 0.01 compared with the model (MOD) group.

Figure 4. Specnuezhenide (SPN) upregulates the protein expression of bile acid (BA) enzymes in D−galactose (D−gal) mice. (A) Immunohistochemistry (IHC) analysis of cytochrome P4507A1 (CYP7A1), cytochrome P45027A1 (CYP27A1), cytochrome P4507B1 (CYP7B1), and cytochrome P4508B1 (CYP8B1) in the liver (black arrows, n = 5). (B) The levels of CYP7A1, CYP27A1, CYP7B1, and CYP8B1 were analyzed using western blotting. The densitometric quantification of CYP7A1, CYP27A1, CYP7B1, and CYP8B1 is shown in the right panels (n = 3). Data are expressed as mean ± SD. ** p < 0.01 compared with the model (MOD) group.