Cytochrome P450 2D6 Antibody - #DF4758

Fig. 6. Combination of pirfenidone and SB525334 reduced the senescence of hepatocytes in mice with CE. (A) Both pirfenidone and combined medication potentially reduced the expression of p16, p21 and p53 (markers of hepatocyte ageing). However, SB525334 could only reduce the expression of p21 and p53. (B) and (C) Immunofluorescence showing that combined medication could reduce the expression of p21 and p53 more effectively compared to both individual drugs and controls. (D) SA-β-Gal staining revealing that combined treatment could more effectively alleviate senescence in hepatocytes compared to the individual drugs. All the images were obtained from a microscopic imaging system at 40× magnification. Data are representative of one of three independent experiments (n = 3–4 mice per group). One-way ANOVA was used for analysis in (A), bars represent the mean ± S.E.M. (ns, not significant; *P < 0.05, **P < 0.01, ***P < 0.001)