产品描述

来源:
Mouse
应用:
ELISA 1:10000, WB 1:500-1:2000, IHC 1:200-1:1000, IF/ICC 1:200-1:1000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human
克隆:
Monoclonal [AFB1620]
特异性:
CHK2 antibody detects endogenous levels of total CHK2.
RRID:
AB_2833644
引用格式: Affinity Biosciences Cat# BF0577, RRID:AB_2833644.
偶联:
Unconjugated.
纯化:
Affinity-chromatography.
保存:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

CDS 1; Cds1; Cds1 homolog; Checkpoint kinase 2; Checkpoint like protein CHK2; CHEK 2; Chek2; Chk 2; CHK2 checkpoint homolog (S. pombe); CHK2 checkpoint homolog; CHK2_HUMAN; hCds1; HuCds 1; LFS 2; LFS2; PP1425; RAD 53; RAD53; Rad53 homolog; Serine/threonine protein kinase Chk2; Serine/threonine-protein kinase Chk2;

抗原和靶标

免疫原:

Purified recombinant fragment of human CHK2 expressed in E. Coli.

Uniprot:
基因/基因ID:
表达:
O96017 CHK2_HUMAN:

High expression is found in testis, spleen, colon and peripheral blood leukocytes. Low expression is found in other tissues.

描述:
CHK2: CHK2 checkpoint homolog (S. pombe). In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants encoding different isoforms have been found for this gene.
序列:
MSRESDVEAQQSHGSSACSQPHGSVTQSQGSSSQSQGISSSSTSTMPNSSQSSHSSSGTLSSLETVSTQELYSIPEDQEPEDQEPEEPTPAPWARLWALQDGFANLECVNDNYWFGRDKSCEYCFDEPLLKRTDKYRTYSKKHFRIFREVGPKNSYIAYIEDHSGNGTFVNTELVGKGKRRPLNNNSEIALSLSRNKVFVFFDLTVDDQSVYPKALRDEYIMSKTLGSGACGEVKLAFERKTCKKVAIKIISKRKFAIGSAREADPALNVETEIEILKKLNHPCIIKIKNFFDAEDYYIVLELMEGGELFDKVVGNKRLKEATCKLYFYQMLLAVQYLHENGIIHRDLKPENVLLSSQEEDCLIKITDFGHSKILGETSLMRTLCGTPTYLAPEVLVSVGTAGYNRAVDCWSLGVILFICLSGYPPFSEHRTQVSLKDQITSGKYNFIPEVWAEVSEKALDLVKKLLVVDPKARFTTEEALRHPWLQDEDMKRKFQDLLSEENESTALPQVLAQPSTSRKRPREGEAEGAETTKRPAVCAAVL

翻译修饰 - O96017 作为底物

Site PTM Type Enzyme
S12 Phosphorylation
S15 Phosphorylation
S19 Phosphorylation O96017 (CHEK2) , Q13315 (ATM)
S24 Phosphorylation
T26 Phosphorylation P53350 (PLK1) , Q13315 (ATM)
S28 Phosphorylation Q13315 (ATM)
S33 Phosphorylation Q13315 (ATM) , O96017 (CHEK2)
S35 Phosphorylation O96017 (CHEK2) , Q13315 (ATM)
S39 Phosphorylation
S40 Phosphorylation
S41 Phosphorylation
S42 Phosphorylation
T43 Phosphorylation
S44 Phosphorylation
T45 Phosphorylation
S50 Phosphorylation Q13315 (ATM)
S52 Phosphorylation
S55 Phosphorylation
S62 Phosphorylation Q9H4B4 (PLK3)
T65 Phosphorylation
S67 Phosphorylation
T68 Phosphorylation P33981 (TTK) , P78527 (PRKDC) , Q13535 (ATR) , P53350 (PLK1) , Q9NYL2 (MAP3K20) , Q13315 (ATM) , O96017 (CHEK2) , Q683Z8 (CHK2)
S73 Phosphorylation Q9H4B4 (PLK3)
K119 Ubiquitination
S120 Phosphorylation O96017 (CHEK2)
K131 Ubiquitination
S140 Phosphorylation O96017 (CHEK2)
S164 Phosphorylation P53350 (PLK1)
T205 Phosphorylation P53350 (PLK1)
S210 Phosphorylation P53350 (PLK1)
K224 Ubiquitination
T225 Phosphorylation O96017 (CHEK2)
K235 Acetylation
S260 Phosphorylation O96017 (CHEK2)
K279 Ubiquitination
K287 Ubiquitination
S357 Phosphorylation Q13315 (ATM)
S372 Phosphorylation
K373 Ubiquitination
T378 Phosphorylation
S379 Phosphorylation O96017 (CHEK2)
T383 Phosphorylation P78527 (PRKDC) , O96017 (CHEK2) , Q683Z8 (CHK2)
T387 Phosphorylation Q683Z8 (CHK2) , P78527 (PRKDC) , O96017 (CHEK2)
T389 Phosphorylation
Y390 Phosphorylation
T432 Phosphorylation O96017 (CHEK2)
S435 Phosphorylation O96017 (CHEK2)
K437 Ubiquitination
K444 Ubiquitination
S456 Phosphorylation
K458 Ubiquitination
K472 Ubiquitination
K494 Ubiquitination
S516 Phosphorylation O96017 (CHEK2) , Q683Z8 (CHK2)
T517 Phosphorylation
C539 S-Nitrosylation

翻译修饰 - O96017 作为激酶

Substrate Site Source
O00716 (E2F3) S124 Uniprot
O15151 (MDM4) S342 Uniprot
O15151-1 (MDM4) S367 Uniprot
O43255 (SIAH2) T26 Uniprot
O43255 (SIAH2) S28 Uniprot
O43255 (SIAH2) S68 Uniprot
O43255 (SIAH2) T119 Uniprot
O60673 (REV3L) S1075 Uniprot
O96017 (CHEK2) S19 Uniprot
O96017 (CHEK2) S33 Uniprot
O96017 (CHEK2) S35 Uniprot
O96017-12 (CHEK2) T68 Uniprot
O96017 (CHEK2) S120 Uniprot
O96017 (CHEK2) S140 Uniprot
O96017 (CHEK2) T225 Uniprot
O96017 (CHEK2) S260 Uniprot
O96017-12 (CHEK2) T354 Uniprot
O96017-12 (CHEK2) T358 Uniprot
O96017 (CHEK2) S379 Uniprot
O96017 (CHEK2) T383 Uniprot
O96017 (CHEK2) T387 Uniprot
O96017-9 (CHEK2) T426 Uniprot
O96017-9 (CHEK2) T430 Uniprot
O96017 (CHEK2) T432 Uniprot
O96017 (CHEK2) S435 Uniprot
O96017-12 (CHEK2) S487 Uniprot
O96017-1 (CHEK2) S516 Uniprot
O96017-9 (CHEK2) S559 Uniprot
P04637 (TP53) S15 Uniprot
P04637 (TP53) T18 Uniprot
P04637 (TP53) S20 Uniprot
P04637 (TP53) S37 Uniprot
P04637 (TP53) S366 Uniprot
P04637 (TP53) S378 Uniprot
P06400 (RB1) S612 Uniprot
P10636-8 (MAPT) S262 Uniprot
P10636 (MAPT) S579 Uniprot
P16403 (HIST1H1C) S36 Uniprot
P16403 (HIST1H1C) S55 Uniprot
P16403 (HIST1H1C) S89 Uniprot
P16403 (HIST1H1C) T92 Uniprot
P16403 (HIST1H1C) T167 Uniprot
P18887 (XRCC1) S184 Uniprot
P18887 (XRCC1) S210 Uniprot
P18887 (XRCC1) T284 Uniprot
P21127 (CDK11B) S752 Uniprot
P23560 (BDNF) T62 Uniprot
P29590-3 (PML) S117 Uniprot
P30304 (CDC25A) S124 Uniprot
P30304 (CDC25A) S178 Uniprot
P30304-2 (CDC25A) S239 Uniprot
P30304-2 (CDC25A) S253 Uniprot
P30304 (CDC25A) S279 Uniprot
P30304 (CDC25A) S293 Uniprot
P30307 (CDC25C) S216 Uniprot
P33981 (TTK) T288 Uniprot
P38398-8 (BRCA1) S941 Uniprot
P38398-7 (BRCA1) S988 Uniprot
P40337 (VHL) S111 Uniprot
P54132 (BLM) T182 Uniprot
P61289 (PSME3) S247 Uniprot
Q01094 (E2F1) S364 Uniprot
Q08050-2 (FOXM1) S361 Uniprot
Q08050 (FOXM1) S376 Uniprot
Q13263 (TRIM28) S473 Uniprot
Q13972 (RASGRF1) S287 Uniprot
Q15717 (ELAVL1) S88 Uniprot
Q15717 (ELAVL1) S100 Uniprot
Q15717 (ELAVL1) T118 Uniprot
Q9NRM7 (LATS2) S172 Uniprot
Q9NRM7 (LATS2) S380 Uniprot
Q9NRM7 (LATS2) S408 Uniprot
Q9NRM7 (LATS2) S446 Uniprot
Q9NY61 (AATF) S141 Uniprot
Q9NY61 (AATF) S143 Uniprot
Q9NY61 (AATF) S477 Uniprot
Q9NY61 (AATF) S510 Uniprot
Q9UQ88 (CDK11A) S740 Uniprot

研究背景

功能:

Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest, activation of DNA repair and apoptosis in response to the presence of DNA double-strand breaks. May also negatively regulate cell cycle progression during unperturbed cell cycles. Following activation, phosphorylates numerous effectors preferentially at the consensus sequence [L-X-R-X-X-S/T]. Regulates cell cycle checkpoint arrest through phosphorylation of CDC25A, CDC25B and CDC25C, inhibiting their activity. Inhibition of CDC25 phosphatase activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. May also phosphorylate NEK6 which is involved in G2/M cell cycle arrest. Regulates DNA repair through phosphorylation of BRCA2, enhancing the association of RAD51 with chromatin which promotes DNA repair by homologous recombination. Also stimulates the transcription of genes involved in DNA repair (including BRCA2) through the phosphorylation and activation of the transcription factor FOXM1. Regulates apoptosis through the phosphorylation of p53/TP53, MDM4 and PML. Phosphorylation of p53/TP53 at 'Ser-20' by CHEK2 may alleviate inhibition by MDM2, leading to accumulation of active p53/TP53. Phosphorylation of MDM4 may also reduce degradation of p53/TP53. Also controls the transcription of pro-apoptotic genes through phosphorylation of the transcription factor E2F1. Tumor suppressor, it may also have a DNA damage-independent function in mitotic spindle assembly by phosphorylating BRCA1. Its absence may be a cause of the chromosomal instability observed in some cancer cells. Promotes the CCAR2-SIRT1 association and is required for CCAR2-mediated SIRT1 inhibition.

翻译修饰:

Phosphorylated. Phosphorylated at Ser-73 by PLK3 in response to DNA damage, promoting phosphorylation at Thr-68 by ATM and the G2/M transition checkpoint. Phosphorylation at Thr-68 induces homodimerization. Autophosphorylates at Thr-383 and Thr-387 in the T-loop/activation segment upon dimerization to become fully active and phosphorylate its substrates like for instance CDC25C. DNA damage-induced autophosphorylation at Ser-379 induces CUL1-mediated ubiquitination and regulates the pro-apoptotic function. Phosphorylation at Ser-456 also regulates ubiquitination. Phosphorylated by PLK4.

Ubiquitinated. CUL1-mediated ubiquitination regulates the pro-apoptotic function. Ubiquitination may also regulate protein stability. Ubiquitinated by RNF8 via 'Lys-48'-linked ubiquitination.

细胞定位:

Nucleus.
Note: Isoform 10 is present throughout the cell.

Nucleus.

Nucleus.

Nucleus.

Nucleus.

Nucleus>PML body. Nucleus>Nucleoplasm.
Note: Recruited into PML bodies together with TP53.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

High expression is found in testis, spleen, colon and peripheral blood leukocytes. Low expression is found in other tissues.

亚基结构:

Homodimer. Homodimerization is part of the activation process but the dimer may dissociate following activation. Interacts with PML. Interacts with TP53. Interacts with RB1; phosphorylates RB1. Interacts with BRCA1. Interacts (phosphorylated at Thr-68) with MDC1; requires ATM-mediated phosphorylation of CHEK2. Interacts with TP53BP1; modulates CHEK2 phosphorylation at Thr-68 in response to ionizing radiation. Interacts with CDC25A; phosphorylates CDC25A and mediates its degradation in response to ionizing radiation. Interacts with CUL1; mediates CHEK2 ubiquitination and regulation. Interacts with CDKN2AIP. Interacts (via protein kinase domain) with CCAR2 (via N-terminus). Interacts with SIRT1.

蛋白家族:

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CHK2 subfamily.

研究领域

· Cellular Processes > Cell growth and death > Cell cycle.   (View pathway)

· Cellular Processes > Cell growth and death > p53 signaling pathway.   (View pathway)

· Cellular Processes > Cell growth and death > Cellular senescence.   (View pathway)

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

限制条款

产品的规格、报价、验证数据请以官网为准,官网链接:www.affbiotech.com | www.affbiotech.cn(简体中文)| www.affbiotech.jp(日本語)

产品的数据信息为Affinity所有,未经授权不得收集Affinity官网数据或资料用于商业用途,对抄袭产品数据的行为我们将保留诉诸法律的权利。

产品相关数据会因产品批次、产品检测情况随时调整,如您已订购该产品,请以订购时随货说明书为准,否则请以官网内容为准,官网内容有改动时恕不另行通知。

Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时,请您在Affinity官方渠道提交售后申请。

产品仅供科学研究使用。不用于诊断和治疗。 

产品未经授权不得转售。

Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.