FGF13 Antibody - #DF4699

Figure 6. Regulation of FGF13 expression by lncRNA01103 in the SDH of BCP rats. (A) Western blots for monitoring FGF13 expression changes with BCP progression. ß-Actin served as loading control. Quantifications of FGF13 intensities, normalized to ß-Actin, are shown below. Bars represent the means ± SEM of Bars represent the means ± SEM of independent biological replicates (n = 4). Values of individual experiments are indicated as dots. P-values were calculated using the one-way ANOVA (**P < 0.01; ***P < 0.001). (B) Western blots for monitoring changes in FGF13 expression after inhibition of lncRNA01103. ß-Actin served as loading control. Quantifications of FGF13 intensities, normalized to ß-Actin, are shown below. Bars represent the means ± SEM of independent biological replicates (n = 4). Values of individual experiments are indicated as dots. P-values were calculated using the one- way ANOVA (***P < 0.001). (C) RNA pull down for monitoring lncRNA01103- probe detected Fgf13 genes. Bars represent the means ± SEM of independent biological replicates (n = 3). Values of individual experiments are indicated as dots. P- values were calculated using the one-way ANOVA (**P < 0.01; ***P < 0.001). (D-E) Immunofluorescence experiments showing SDH FGF13 expression after intrathecal injection of siRNA01103 in BCP rats (D). Quantifications of FGF13 mean immunofluorescence intensity are shown E. Bars represent the means ± SEM of independent biological replicates (n = 3). Values of individual experiments are indicated as dots. P-values were calculated using the one-way ANOVA (***P < 0.001). (F) Immunofluorescence staining of FGF13 and NeuN, Iba-1, and GFAP in the SDH of BCP rats at 12 d. Arrows indicate representative examples of double- labeled cells. (G) FISH (lncRNA01103, red, FGF13, green) staining in the SDH of BCP rats on postoperative day 12.