产品: SPON2 抗体
货号: DF4682
描述: Rabbit polyclonal antibody to SPON2
应用: WB IHC IF/ICC
文献验证: IHC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Chicken, Xenopus
分子量: 37 KD; 36kD(Calculated).
蛋白号: Q9BUD6
RRID: AB_2837033

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
预测:
Pig(90%), Bovine(%), Horse(%), Chicken(%), Xenopus(%)
克隆:
Polyclonal
特异性:
SPON2 Antibody detects endogenous levels of total SPON2.
RRID:
AB_2837033
引用格式: Affinity Biosciences Cat# DF4682, RRID:AB_2837033.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Differentially expressed in cancerous and non-cancerous lung cells 1; DIL 1; DIL-1; DIL1; DKFZp686G21139; FLJ16313; FLJ22401; FLJ34460; M spondin; Mindin; ORF Names:;antibody;SPON 2; Spon2; SPON2_HUMAN; Spondin 2; Spondin 2 extracellular matrix protein; Spondin-2;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
Q9BUD6 SPON2_HUMAN:

Expressed in normal lung tissue but not in lung carcinoma cell lines.

序列:
MENPSPAAALGKALCALLLATLGAAGQPLGGESICSARALAKYSITFTGKWSQTAFPKQYPLFRPPAQWSSLLGAAHSSDYSMWRKNQYVSNGLRDFAERGEAWALMKEIEAAGEALQSVHEVFSAPAVPSGTGQTSAELEVQRRHSLVSFVVRIVPSPDWFVGVDSLDLCDGDRWREQAALDLYPYDAGTDSGFTFSSPNFATIPQDTVTEITSSSPSHPANSFYYPRLKALPPIARVTLVRLRQSPRAFIPPAPVLPSRDNEIVDSASVPETPLDCEVSLWSSWGLCGGHCGRLGTKSRTRYVRVQPANNGSPCPELEEEAECVPDNCV

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
90
Horse
90
Bovine
90
Xenopus
80
Chicken
80
Sheep
0
Dog
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

功能:

Cell adhesion protein that promotes adhesion and outgrowth of hippocampal embryonic neurons. Binds directly to bacteria and their components and functions as an opsonin for macrophage phagocytosis of bacteria. Essential in the initiation of the innate immune response and represents a unique pattern-recognition molecule in the ECM for microbial pathogens (By similarity). Binds bacterial lipopolysaccharide (LPS).

细胞定位:

Secreted>Extracellular space>Extracellular matrix.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed in normal lung tissue but not in lung carcinoma cell lines.

亚基结构:

Monomer. Interacts with integrin.

文献引用

1). Upregulation of Spondin-2 protein expression correlates with poor prognosis in hepatocellular carcinoma. The Journal of international medical research, 2019 (PubMed: 30318967) [IF=1.4]

Application: IHC    Species: Human    Sample: HCC and liver tissue

Figure 2. Expression of SPON2 in HCC and normal liver tissue. (a) qRT-PCR analysis of SPON2 mRNA expression in 20 paired HCC and matched non-cancerous liver tissues. (b) TMA-IHC analysis of SPON2 protein expression in 107 paired HCC and matched non-cancerous liver tissues.

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