CACNA1H Antibody - #DF4615

Figure 4 Comparisons of protein expression and whole‐cell current densities of T‐type VDCCs in cerebral VSMCs isolated from CON and SUS rats. A‐B, The protein expressions of T‐type CaV3.1 and CaV3.2 channels were examined by Western blotting. GAPDH was used as loading control (n = 10/group). C, mRNA levels of T‐type CaV3.1 channels were assessed by qRT‐PCR analysis. GAPDH was used for normalization (n = 10/group). D, Inward whole‐cell currents were recorded from isolated cerebral VSMCs at −80 mV and −40 mV holding potentials, respectively. E, Whole‐cell currents recorded from cerebral VSMCs with the extracellular application of 0.1 μmol/L Nifedipine and 5 μmol/L Mibefradil in the presence of 0.1 μmol/L Nifedipine. Cumulative I‐V curves are shown in the right panel. F, Whole‐cell currents recorded at −80 mV holding potential in the presence of 0.1 μmol/L Nifedipine were used as the representative T‐type CaV3.1 channels currents. The values are expressed as means ± SEM with the number of cells recorded in parentheses. Data were presented as mean ± SEM. *P < .05 vs control or Baseline, # P < .05 vs 0.1 μmol/L Nifedipine (analysed by Student's t test in B, C, D and F; analysed by ANOVA in E)