产品: UGDH 抗体
货号: DF4603
描述: Rabbit polyclonal antibody to UGDH
应用: WB IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 55 KD; 55kD(Calculated).
蛋白号: O60701
RRID: AB_2836967

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 50ul RMB¥ 1250 现货
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产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
克隆:
Polyclonal
特异性:
UGDH Antibody detects endogenous levels of total UGDH.
RRID:
AB_2836967
引用格式: Affinity Biosciences Cat# DF4603, RRID:AB_2836967.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

GDH; UDP Glc dehydrogenase; UDP GlcDH; UDP glucose 6 dehydrogenase; UDP-Glc dehydrogenase; UDP-GlcDH; UDP-glucose 6-dehydrogenase; UDP-glucose dehydrogenase; UDPGDH; UGD; Ugdh; UGDH_HUMAN; Uridine diphospho glucose dehydrogenase;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
O60701 UGDH_HUMAN:

Detected in heart, placenta, liver, pancreas, spleen, thymus, prostate, ovary, small intestine and colon (PubMed:9737970). Widely expressed (PubMed:9737970).

序列:
MFEIKKICCIGAGYVGGPTCSVIAHMCPEIRVTVVDVNESRINAWNSPTLPIYEPGLKEVVESCRGKNLFFSTNIDDAIKEADLVFISVNTPTKTYGMGKGRAADLKYIEACARRIVQNSNGYKIVTEKSTVPVRAAESIRRIFDANTKPNLNLQVLSNPEFLAEGTAIKDLKNPDRVLIGGDETPEGQRAVQALCAVYEHWVPREKILTTNTWSSELSKLAANAFLAQRISSINSISALCEATGADVEEVATAIGMDQRIGNKFLKASVGFGGSCFQKDVLNLVYLCEALNLPEVARYWQQVIDMNDYQRRRFASRIIDSLFNTVTDKKIAILGFAFKKDTGDTRESSSIYISKYLMDEGAHLHIYDPKVPREQIVVDLSHPGVSEDDQVSRLVTISKDPYEACDGAHAVVICTEWDMFKELDYERIHKKMLKPAFIFDGRRVLDGLHNELQTIGFQIETIGKKVSSKRIPYAPSGEIPKFSLQDPPNKKPKV

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
79
Zebrafish
73
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - O60701 作为底物

Site PTM Type Enzyme
S40 Phosphorylation
S47 Phosphorylation
Y53 Phosphorylation
K67 Ubiquitination
K80 Ubiquitination
S88 Phosphorylation
T91 Phosphorylation
K100 Ubiquitination
K107 Acetylation
K107 Ubiquitination
Y108 Phosphorylation
K124 Ubiquitination
K129 Ubiquitination
K149 Ubiquitination
K170 Ubiquitination
K173 Acetylation
K173 Ubiquitination
T185 Phosphorylation
Y199 Phosphorylation
K207 Ubiquitination
K264 Ubiquitination
K267 Ubiquitination
S275 Phosphorylation
Y286 Phosphorylation
Y309 Phosphorylation
S316 Phosphorylation
S321 Phosphorylation
T327 Phosphorylation
K339 Acetylation
Y352 Phosphorylation
S354 Phosphorylation
K370 Ubiquitination
S386 Phosphorylation
K430 Ubiquitination
K434 Acetylation
T461 Phosphorylation P06493 (CDK1)
Y473 Phosphorylation
S476 Phosphorylation
S483 Phosphorylation
K490 Acetylation

研究背景

功能:

Catalyzes the formation of UDP-alpha-D-glucuronate, a constituent of complex glycosaminoglycans. Required for the biosynthesis of chondroitin sulfate and heparan sulfate. Required for embryonic development via its role in the biosynthesis of glycosaminoglycans (By similarity).

组织特异性:

Detected in heart, placenta, liver, pancreas, spleen, thymus, prostate, ovary, small intestine and colon. Widely expressed.

亚基结构:

Homohexamer.

蛋白家族:

The protein goes through several conformation states during the reaction cycle, giving rise to hysteresis. In the initial state, the ligand-free protein is in an inactive conformation (E*). Substrate binding triggers a change to the active conformation (E). UDP-xylose binding triggers the transition to a distinct, inhibited conformation. The presence of an intrinsically disordered C-terminus promotes a more dynamic protein structure and favors a conformation with high affinity for UPD-xylose.

The allosteric switch region moves by about 5 Angstroms when UDP-xylose is bound, and occupies part of the UDP-glucose binding site. At the same time it promotes domain movements that disrupt the active hexameric ring structure and lead to the formation of a horseshoe-shaped, inactive hexamer.

Belongs to the UDP-glucose/GDP-mannose dehydrogenase family.

研究领域

· Metabolism > Carbohydrate metabolism > Pentose and glucuronate interconversions.

· Metabolism > Carbohydrate metabolism > Ascorbate and aldarate metabolism.

· Metabolism > Carbohydrate metabolism > Amino sugar and nucleotide sugar metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

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