| 产品: | SH3GL2 抗体 |
| 货号: | DF4505 |
| 描述: | Rabbit polyclonal antibody to SH3GL2 |
| 应用: | WB IF/ICC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Bovine, Horse, Sheep, Dog, Chicken, Xenopus |
| 分子量: | 39 KD; 40kD(Calculated). |
| 蛋白号: | Q99962 |
| RRID: | AB_2836856 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF4505, RRID:AB_2836856.
展开/折叠
bA335L15.1 (SH3 domain GRB2 like 2); CNSA2; EEN B1; EEN-B1; Endophilin 1; Endophilin A1; Endophilin A1 BAR domain; Endophilin-1; Endophilin-A1; FLJ20276; FLJ25015; OTTHUMP00000021084; SH3 domain containing GRB2 like protein 2; SH3 domain GRB2 like 2; SH3 domain protein 2A; SH3 domain-containing GRB2-like protein 2; SH3D2A; SH3G2_HUMAN; Sh3gl2; Sh3p4; SH3PA;
抗原和靶标
- Q99962 SH3G2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSVAGLKKQFHKATQKVSEKVGGAEGTKLDDDFKEMERKVDVTSRAVMEIMTKTIEYLQPNPASRAKLSMINTMSKIRGQEKGPGYPQAEALLAEAMLKFGRELGDDCNFGPALGEVGEAMRELSEVKDSLDIEVKQNFIDPLQNLHDKDLREIQHHLKKLEGRRLDFDYKKKRQGKIPDEELRQALEKFDESKEIAESSMFNLLEMDIEQVSQLSALVQAQLEYHKQAVQILQQVTVRLEERIRQASSQPRREYQPKPRMSLEFPTGDSTQPNGGLSHTGTPKPSGVQMDQPCCRALYDFEPENEGELGFKEGDIITLTNQIDENWYEGMLHGHSGFFPINYVEILVALPH
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q99962 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Phosphorylation | Uniprot | |
| T14 | Phosphorylation | O75116 (ROCK2) | Uniprot |
| S75 | Phosphorylation | Q5S007 (LRRK2) | Uniprot |
| K76 | Acetylation | Uniprot | |
| K149 | Ubiquitination | Uniprot | |
| K159 | Ubiquitination | Uniprot | |
| K177 | Ubiquitination | Uniprot | |
| R184 | Methylation | Uniprot | |
| S249 | Phosphorylation | Uniprot |
研究背景
Implicated in synaptic vesicle endocytosis. May recruit other proteins to membranes with high curvature. Required for BDNF-dependent dendrite outgrowth. Cooperates with SH3GL2 to mediate BDNF-NTRK2 early endocytic trafficking and signaling from early endosomes.
Cytoplasm. Membrane>Peripheral membrane protein. Early endosome. Cell junction>Synapse>Presynapse.
Brain, mostly in frontal cortex. Expressed at high level in fetal cerebellum.
Monomer; in cytoplasm. Homodimer; when associated with membranes (By similarity). Interacts with OPHN1 (By similarity). Interacts with SYNJ1. Interacts with DNM1 (By similarity). Interacts with MAP4K3; the interaction appears to regulate MAP4K3-mediated JNK activation (By similarity). Interacts with PDCD6IP. Interacts with ATX2. Interacts with ADAM9 and ADAM15 cytoplasmic tails. Interacts with BIN2. Interacts with TMEM108 (By similarity). Interacts with ADGRB2.
An N-terminal amphipathic helix, the BAR domain and a second amphipathic helix inserted into helix 1 of the BAR domain (N-BAR domain) induce membrane curvature and bind curved membranes. The BAR domain dimer forms a rigid crescent shaped bundle of helices with the pair of second amphipathic helices protruding towards the membrane-binding surface.
Belongs to the endophilin family.
研究领域
· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)
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