产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF4476, RRID:AB_2836827.
展开/折叠
2810468K05Rik; AU020252; D130018F14Rik; EC 2.7.11.1; FLJ14314; hKFC B; hKFC-B; KIAA1361; Kinase from chicken homolog B; MAP3K16; MARK kinase; MARKK; MGC29021; Microtubule affinity regulating kinase kinase; mKIAA1361; Prostate-derived sterile 20-like kinase 2; PSK2; Serine/threonine kinase TAO1; Serine/threonine protein kinase TAO1; Serine/threonine protein kinase TAO1 homolog; Serine/threonine-protein kinase TAO1; STE20 like kinase; STE20 like kinase PSK2; STE20-like kinase PSK2; TAO kinase 1; Taok1; TAOK1_HUMAN; Thousand and one amino acid protein 1;
抗原和靶标
Highly expressed in the testis, and to a lower extent also expressed in brain, placenta, colon and skeletal muscle.
- Q7L7X3 TAOK1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPSTNRAGSLKDPEIAELFFKEDPEKLFTDLREIGHGSFGAVYFARDVRTNEVVAIKKMSYSGKQSTEKWQDIIKEVKFLQRIKHPNSIEYKGCYLREHTAWLVMEYCLGSASDLLEVHKKPLQEVEIAAITHGALQGLAYLHSHTMIHRDIKAGNILLTEPGQVKLADFGSASMASPANSFVGTPYWMAPEVILAMDEGQYDGKVDVWSLGITCIELAERKPPLFNMNAMSALYHIAQNESPTLQSNEWSDYFRNFVDSCLQKIPQDRPTSEELLKHIFVLRERPETVLIDLIQRTKDAVRELDNLQYRKMKKLLFQEAHNGPAVEAQEEEEEQDHGVGRTGTVNSVGSNQSIPSMSISASSQSSSVNSLPDVSDDKSELDMMEGDHTVMSNSSVIHLKPEEENYREEGDPRTRASDPQSPPQVSRHKSHYRNREHFATIRTASLVTRQMQEHEQDSELREQMSGYKRMRRQHQKQLMTLENKLKAEMDEHRLRLDKDLETQRNNFAAEMEKLIKKHQAAMEKEAKVMSNEEKKFQQHIQAQQKKELNSFLESQKREYKLRKEQLKEELNENQSTPKKEKQEWLSKQKENIQHFQAEEEANLLRRQRQYLELECRRFKRRMLLGRHNLEQDLVREELNKRQTQKDLEHAMLLRQHESMQELEFRHLNTIQKMRCELIRLQHQTELTNQLEYNKRRERELRRKHVMEVRQQPKSLKSKELQIKKQFQDTCKIQTRQYKALRNHLLETTPKSEHKAVLKRLKEEQTRKLAILAEQYDHSINEMLSTQALRLDEAQEAECQVLKMQLQQELELLNAYQSKIKMQAEAQHDRELRELEQRVSLRRALLEQKIEEEMLALQNERTERIRSLLERQAREIEAFDSESMRLGFSNMVLSNLSPEAFSHSYPGASGWSHNPTGGPGPHWGHPMGGPPQAWGHPMQGGPQPWGHPSGPMQGVPRGSSMGVRNSPQALRRTASGGRTEQGMSRSTSVTSQISNGSHMSYT
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q7L7X3 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S9 | Phosphorylation | Uniprot | |
| K21 | Ubiquitination | Uniprot | |
| Y43 | Phosphorylation | Uniprot | |
| S66 | Phosphorylation | Uniprot | |
| K84 | Ubiquitination | Uniprot | |
| Y107 | Phosphorylation | Uniprot | |
| S181 | Phosphorylation | Uniprot | |
| T185 | Phosphorylation | Uniprot | |
| R269 | Methylation | Uniprot | |
| Y309 | Phosphorylation | Uniprot | |
| Y406 | Phosphorylation | Uniprot | |
| S417 | Phosphorylation | Uniprot | |
| S421 | Phosphorylation | Uniprot | |
| S426 | Phosphorylation | Uniprot | |
| Y432 | Phosphorylation | Uniprot | |
| T440 | Phosphorylation | Uniprot | |
| S445 | Phosphorylation | Uniprot | |
| S458 | Phosphorylation | Uniprot | |
| T480 | Phosphorylation | Uniprot | |
| T502 | Phosphorylation | Uniprot | |
| K546 | Acetylation | Uniprot | |
| S554 | Phosphorylation | Uniprot | |
| T576 | Phosphorylation | Uniprot | |
| K587 | Ubiquitination | Uniprot | |
| Y610 | Phosphorylation | Uniprot | |
| T643 | Phosphorylation | Q13315 (ATM) | Uniprot |
| T669 | Phosphorylation | Uniprot | |
| S714 | Phosphorylation | Uniprot | |
| K718 | Ubiquitination | Uniprot | |
| T734 | Phosphorylation | Uniprot | |
| K750 | Ubiquitination | Uniprot | |
| T785 | Phosphorylation | Q13315 (ATM) | Uniprot |
| K818 | Ubiquitination | Uniprot | |
| S965 | Phosphorylation | Uniprot | |
| S974 | Phosphorylation | Uniprot | |
| S987 | Phosphorylation | Uniprot | |
| S990 | Phosphorylation | Q13315 (ATM) | Uniprot |
| S993 | Phosphorylation | Uniprot | |
| T1001 | Phosphorylation | Uniprot |
翻译修饰 - Q7L7X3 作为激酶
研究背景
Serine/threonine-protein kinase involved in various processes such as p38/MAPK14 stress-activated MAPK cascade, DNA damage response and regulation of cytoskeleton stability. Phosphorylates MAP2K3, MAP2K6 and MARK2. Acts as an activator of the p38/MAPK14 stress-activated MAPK cascade by mediating phosphorylation and subsequent activation of the upstream MAP2K3 and MAP2K6 kinases. Involved in G-protein coupled receptor signaling to p38/MAPK14. In response to DNA damage, involved in the G2/M transition DNA damage checkpoint by activating the p38/MAPK14 stress-activated MAPK cascade, probably by mediating phosphorylation of MAP2K3 and MAP2K6. Acts as a regulator of cytoskeleton stability by phosphorylating 'Thr-208' of MARK2, leading to activate MARK2 kinase activity and subsequent phosphorylation and detachment of MAPT/TAU from microtubules. Also acts as a regulator of apoptosis: regulates apoptotic morphological changes, including cell contraction, membrane blebbing and apoptotic bodies formation via activation of the MAPK8/JNK cascade.
Proteolytically processed by caspase-3 (CASP3).
Autophosphorylated (By similarity). Phosphorylated by ATM in response to DNA damage. Phosphorylated by LRRK2.
Cytoplasm.
Highly expressed in the testis, and to a lower extent also expressed in brain, placenta, colon and skeletal muscle.
Self-associates. Interacts with MAP2K3 (By similarity). Interacts with SPRED1 and TESK1. Interacts with MAP3K7.
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
研究领域
· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)
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