NEK7 Antibody - #DF4467

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产品: NEK7 抗体
货号: DF4467
描述: Rabbit polyclonal antibody to NEK7
应用: WB IHC IF/ICC
文献验证: WB, IHC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
蛋白号: Q8TDX7
RRID: AB_2836822

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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实验方案
WB Handbook
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IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
NEK7 Antibody detects endogenous levels of total NEK7.
RRID:
AB_2836822
引用格式: Affinity Biosciences Cat# DF4467, RRID:AB_2836822.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

EC 2.7.11.1; NEK7; NEK7_HUMAN; Never in mitosis A-related kinase 7; Never in mitosis gene a-related kinase 7; NIMA (never in mitosis gene a) related kinase 7; NIMA (never in mitosis gene a)-related expressed kinase 7; NimA related protein kinase 7; NIMA-related kinase 7; NimA-related protein kinase 7; Serine/threonine-protein kinase Nek7;

抗原和靶标

免疫原:

A synthesized peptide derived from human NEK7, corresponding to a region within the internal amino acids.

基因/基因ID:
NEK7(140609)

研究领域

· Organismal Systems > Immune system > NOD-like receptor signaling pathway.   (View pathway)

文献引用

1). Erianin isolated from Dendrobium huoshanense alleviated neuroinflammation in MPTP-induced Parkinson's disease model via NF-κB/NLRP3 pathway. Journal of ethnopharmacology, 2025 (PubMed: 40074095) [IF=4.8]
2). Total tanshinones protect against acute lung injury through the PLCγ2/NLRP3 inflammasome signaling pathway. JOURNAL OF ETHNOPHARMACOLOGY, 2023 (PubMed: 37121449) [IF=4.8]
3). Quercetin-induced pyroptosis in colon cancer through NEK7-mediated NLRP3 inflammasome-GSDMD signaling pathway activation. American journal of cancer research, 2024 (PubMed: 38590424) [IF=3.6]

Application: WB    Species: Human    Sample: colon cancer tissues

Figure 7 Downregulated expression of NEK7 in human colon cancer tissues and correlated with NLRP3 expression. A. Gene correlation between NLRP3 and NEK7 in colon cancer investigated using the TIMER2.0 database based on Spearman’s correlation analysis. Rho represents the correlation index. B. Influence of NEK7 knockdown on NLRP3 protein expression determined using western blotting (n=3, where “n” represents the number of independent repeated experiments). Values are presented as mean ± SD. *P
4). Attenuation of ROS/Chloride Efflux-Mediated NLRP3 Inflammasome Activation Contributes to Alleviation of Diabetic Cardiomyopathy in Rats after Sleeve Gastrectomy. Oxidative Medicine and Cellular Longevity, 2022 (PubMed: 35498125)

Application: WB    Species: Rat    Sample: diabetic myocardium

"Figure 4 SG attenuates the activation of NLRP3 in diabetic myocardium. (a) Protein levels of NLRP3-related molecules shown by western blot. β-Tubulin was an internal reference control. n = 3 in each group. (b) Relative mRNA levels of Nlrp3-relaterd genes shown by real-time RT-PCR. Tubb3 served as a reference gene. n = 3 in each group. (c) Ratio of caspase-1 p12/procaspase-1. n = 3 in each group. (d) Caspase-1 activity in heart tissues. n = 6 in each group. Immunohistochemical staining of (e) NLRP3, (f) ASC, (g) GSDMD, (h) IL-1β, (i) IL-18, and (j) NEK7. Scale bar, 50 μm. (k) Double-labeling immunofluorescence of NLRP3 and CD68. Scale bar, 50 μm. Data are presented as mean ± SD. ∗∗P < 0.01 CON vs. SHAM; ##P < 0.01 SG vs. SHAM. n = 3 in each group."

Application: IHC    Species: Rat    Sample: diabetic myocardium

"Figure 4 SG attenuates the activation of NLRP3 in diabetic myocardium. (a) Protein levels of NLRP3-related molecules shown by western blot. β-Tubulin was an internal reference control. n = 3 in each group. (b) Relative mRNA levels of Nlrp3-relaterd genes shown by real-time RT-PCR. Tubb3 served as a reference gene. n = 3 in each group. (c) Ratio of caspase-1 p12/procaspase-1. n = 3 in each group. (d) Caspase-1 activity in heart tissues. n = 6 in each group. Immunohistochemical staining of (e) NLRP3, (f) ASC, (g) GSDMD, (h) IL-1β, (i) IL-18, and (j) NEK7. Scale bar, 50 μm. (k) Double-labeling immunofluorescence of NLRP3 and CD68. Scale bar, 50 μm. Data are presented as mean ± SD. ∗∗P < 0.01 CON vs. SHAM; ##P < 0.01 SG vs. SHAM. n = 3 in each group."

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