产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF4448, RRID:AB_2836803.
展开/折叠
3000002E04Rik; FLJ22251; FYVE finger protein EIP1; FYVE-finger protein EIP1; La binding protein 1; La-binding protein 1; OTTHUMP00000161521; Rab4 interacting protein; Rab4-interacting protein; RABIP4; RUFY1; RUFY1_HUMAN; RUN and FYVE domain containing 1; RUN and FYVE domain-containing protein 1; RUN and FYVE domains containing protein 1; ZFYVE12; Zinc finger FYVE domain containing protein 12; Zinc finger FYVE domain-containing protein 12;
抗原和靶标
- Q96T51 RUFY1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MADREGGCAAGRGRELEPELEPGPGPGSALEPGEEFEIVDRSQLPGPGDLRSATRPRAAEGWSAPILTLARRATGNLSASCGSALRAAAGLGGGDSGDGTARAASKCQMMEERANLMHMMKLSIKVLLQSALSLGRSLDADHAPLQQFFVVMEHCLKHGLKVKKSFIGQNKSFFGPLELVEKLCPEASDIATSVRNLPELKTAVGRGRAWLYLALMQKKLADYLKVLIDNKHLLSEFYEPEALMMEEEGMVIVGLLVGLNVLDANLCLKGEDLDSQVGVIDFSLYLKDVQDLDGGKEHERITDVLDQKNYVEELNRHLSCTVGDLQTKIDGLEKTNSKLQEELSAATDRICSLQEEQQQLREQNELIRERSEKSVEITKQDTKVELETYKQTRQGLDEMYSDVWKQLKEEKKVRLELEKELELQIGMKTEMEIAMKLLEKDTHEKQDTLVALRQQLEEVKAINLQMFHKAQNAESSLQQKNEAITSFEGKTNQVMSSMKQMEERLQHSERARQGAEERSHKLQQELGGRIGALQLQLSQLHEQCSSLEKELKSEKEQRQALQRELQHEKDTSSLLRMELQQVEGLKKELRELQDEKAELQKICEEQEQALQEMGLHLSQSKLKMEDIKEVNQALKGHAWLKDDEATHCRQCEKEFSISRRKHHCRNCGHIFCNTCSSNELALPSYPKPVRVCDSCHTLLLQRCSSTAS
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q96T51 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| R12 | Methylation | Uniprot | |
| R14 | Methylation | Uniprot | |
| S63 | Phosphorylation | Uniprot | |
| T74 | Phosphorylation | Uniprot | |
| S78 | Phosphorylation | Uniprot | |
| S80 | Phosphorylation | Uniprot | |
| S83 | Phosphorylation | Uniprot | |
| S123 | Phosphorylation | Uniprot | |
| K164 | Ubiquitination | Uniprot | |
| S165 | Phosphorylation | Uniprot | |
| K171 | Ubiquitination | Uniprot | |
| S172 | Phosphorylation | Uniprot | |
| K182 | Ubiquitination | Uniprot | |
| C184 | S-Nitrosylation | Uniprot | |
| S188 | Phosphorylation | Uniprot | |
| T192 | Phosphorylation | Uniprot | |
| S193 | Phosphorylation | Uniprot | |
| K225 | Methylation | Uniprot | |
| K308 | Ubiquitination | Uniprot | |
| S319 | Phosphorylation | Uniprot | |
| C320 | S-Nitrosylation | Uniprot | |
| K338 | Ubiquitination | Uniprot | |
| S344 | Phosphorylation | Uniprot | |
| T347 | Phosphorylation | Uniprot | |
| R349 | Methylation | Uniprot | |
| S352 | Phosphorylation | Uniprot | |
| K379 | Acetylation | Uniprot | |
| Y389 | Phosphorylation | P51813 (BMX) | Uniprot |
| K390 | Ubiquitination | Uniprot | |
| Y400 | Phosphorylation | P51813 (BMX) | Uniprot |
| S401 | Phosphorylation | Uniprot | |
| K445 | Ubiquitination | Uniprot | |
| K460 | Ubiquitination | Uniprot | |
| K469 | Ubiquitination | Uniprot | |
| K480 | Ubiquitination | Uniprot | |
| S486 | Phosphorylation | Uniprot | |
| K490 | Ubiquitination | Uniprot | |
| K499 | Ubiquitination | Uniprot | |
| K521 | Ubiquitination | Uniprot | |
| K555 | Methylation | Uniprot | |
| K569 | Ubiquitination | Uniprot | |
| S618 | Phosphorylation | Uniprot | |
| S620 | Phosphorylation | Uniprot | |
| K621 | Ubiquitination | Uniprot | |
| K635 | Ubiquitination | Uniprot | |
| K641 | Ubiquitination | Uniprot | |
| S694 | Phosphorylation | Uniprot |
研究背景
Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in early endosomal trafficking.
Phosphorylation on Tyr-389 and/or Tyr-400 is required for interaction with BMX and endosomal targeting.
Cytoplasm. Early endosome membrane>Peripheral membrane protein.
Broadly expressed, with highest levels in lung, testis, kidney and brain.
Interacts with BMX. May interact with SSB. Interacts with RAB4 and RAB5 that have been activated by GTP-binding.
The FYVE-type zinc finger domain mediates interactions with phosphatidylinositol 3-phosphate in membranes of early endosomes and penetrates bilayers. The FYVE domain insertion into PtdIns(3)P-enriched membranes is substantially increased in acidic conditions.
研究领域
· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)
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