ARHGEF12 Antibody - #DF4432

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产品: ARHGEF12 抗体
货号: DF4432
描述: Rabbit polyclonal antibody to ARHGEF12
应用: WB IF/ICC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
蛋白号: Q9NZN5
RRID: AB_2836787

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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说明书
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
ARHGEF12 Antibody detects endogenous levels of total ARHGEF12.
RRID:
AB_2836787
引用格式: Affinity Biosciences Cat# DF4432, RRID:AB_2836787.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

ARHGC_HUMAN; ARHGEF12; KIAA0382; leukemia-associated rho guanine nucleotide exchange factor; Leukemia-associated RhoGEF; PRO2792; Rho guanine exchange factor (GEF) 12; Rho guanine nucleotide exchange factor (GEF) 12; Rho guanine nucleotide exchange factor 12; Similar to Mouse Lsc oncogene;

抗原和靶标

免疫原:

A synthesized peptide derived from human ARHGEF12, corresponding to a region within the internal amino acids.

基因/基因ID:
ARHGEF12(23365)

研究领域

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Organismal Systems > Circulatory system > Vascular smooth muscle contraction.   (View pathway)

· Organismal Systems > Development > Axon guidance.   (View pathway)

· Organismal Systems > Immune system > Platelet activation.   (View pathway)

文献引用

1). ITRAQ-Based Proteomics Analysis Reveals the Effect of Neoliensinine on KCl-Induced Vascular Smooth Muscle Contraction by Inhibiting Regulatory Light Chain Phosphorylation. Frontiers in Pharmacology, 2019 (PubMed: 31572175) [IF=5.6]

Application: WB    Species: mouse    Sample: mesenteric arteries

FIGURE 4 | Reducing expressions of PLCβ and RhoGEF12 of neoliensinine in mesenteric arteries contracted by KCl. (A) Protein expressions of PLCβ and RhoGEF12 by Western blot
2). MiR-361-5p/abca1 and MiR-196-5p/arhgef12 Axis Involved in γ-Sitosterol Inducing Dual Anti-Proliferative Effects on Bronchial Epithelial Cells of Chronic Obstructive Pulmonary Disease. International Journal of Chronic Obstructive Pulmonary Disease, 2021 (PubMed: 34675500) [IF=2.7]

Application: WB    Species: Human    Sample: H292 and BEAS-2B cells

Figure 3 Effect of GS on regulating miR-196-5p and miR-361-5p expression. (A) After GS treatment, the expressions of miR-196-5p and miR-361-5p were detected in BEAS-2B and H292 cells. (B) Cell proliferation were performed in BEAS-2B cells after GS single or combined with either miR-196-5p mimic or miR-361-5p inhibitor. (C) miR-196-5p and miR-361-5p target mRNAs were matched to GSE130928 profile, respectively. The expressions of bcat1 and arhgef12 of GSE130928 were showed in (D) and the binding motifs of miR-196-5p to arhgef12 and miR-361-5p to bcat1 were showed in (E), respectively. The mRNA (F) and protein levels (G) were detected in H292 and BEAS-2B cells. Shown are representative and expressed as the Means ± S.D. The bars with different superscripts in each panel were significantly different. Experiments were performed in triplicate for each group, *p< 0.05.

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