产品: CD20 抗体
货号: BF0233
描述: Mouse monoclonal antibody to CD20
应用: IHC IF/ICC ELISA
反应: Human
分子量: 33kDa.; 33kD(Calculated).
蛋白号: P11836
RRID: AB_2833614

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 50ul RMB¥ 1800 现货
 100ul RMB¥ 2800 现货

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产品描述

来源:
Mouse
应用:
ELISA 1:10000, IHC 1:200-1:1000, IF/ICC 1:200-1:1000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human
克隆:
Monoclonal [AFB1589]
特异性:
CD20 antibody detects endogenous levels of total CD20.
RRID:
AB_2833614
引用格式: Affinity Biosciences Cat# BF0233, RRID:AB_2833614.
偶联:
Unconjugated.
纯化:
Affinity-chromatography.
保存:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

APY; ATOPY; B lymphocyte antigen CD20; B Lymphocyte Cell Surface Antigen B1; B-lymphocyte antigen CD20; B-lymphocyte cell-surface antigen B1; B-lymphocyte surface antigen B1; B1; Bp 35; Bp35; CD 20; CD20; CD20 antigen; CD20 receptor; CD20_HUMAN; CVID5; Fc epsilon receptor I beta chain; Fc Fragment of IgE high affinity I receptor for beta polypeptide; FCER1B; High affinity immunoglobulin epsilon receptor subunit beta; IgE Fc receptor subunit beta; IGEL; IGER; IGHER; LEU 16; LEU16; leukocyte surface antigen Leu 16; Leukocyte surface antigen Leu-16; Ly44; Membrane spanning 4 domains A1; Membrane spanning 4 domains subfamily A member 2; Membrane-spanning 4-domains subfamily A member 1; MS4A1; MS4A2; S7;

抗原和靶标

免疫原:

Purified recombinant fragment of human CD20 expressed in E. Coli.

Uniprot:
基因/基因ID:
表达:
P11836 CD20_HUMAN:

Expressed on B-cells.

描述:
CD20 (MS4A1, membrane-spanning 4-domains, subfamily A, member 1) is a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. The CD20 antigen is present on human pre B lymphocytes and on B lymphocytes at all stages of maturation, except on plasma cells. Low level expression of the CD20 antigen has been detected on normal T lymphocytes. The CD20 molecule is involved in regulation of B cell differentiation, presumably via its reported function as a Ca++ channel subunit. And it is known to accelerate the G0 to G1 progression induced by IGF-1.
序列:
MTTPRNSVNGTFPAEPMKGPIAMQSGPKPLFRRMSSLVGPTQSFFMRESKTLGAVQIMNGLFHIALGGLLMIPAGIYAPICVTVWYPLWGGIMYIISGSLLAATEKNSRKCLVKGKMIMNSLSLFAAISGMILSIMDILNIKISHFLKMESLNFIRAHTPYINIYNCEPANPSEKNSPSTQYCYSIQSLFLGILSVMLIFAFFQELVIAGIVENEWKRTCSRPKSNIVLLSAEEKKEQTIEIKEEVVGLTETSSQPKNEEDIEIIPIQEEEEEETETNFPEPPQDQESSPIENDSSP

翻译修饰 - P11836 作为底物

Site PTM Type Enzyme
T2 Phosphorylation
T3 Phosphorylation
S7 Phosphorylation
T11 Phosphorylation
S25 Phosphorylation
S35 Phosphorylation
S36 Phosphorylation
T41 Phosphorylation
S121 Phosphorylation
S225 Phosphorylation
S231 Phosphorylation P68400 (CSNK2A1) , P19784 (CSNK2A2)
K235 Ubiquitination
K236 Ubiquitination
K243 Ubiquitination
T250 Phosphorylation P68400 (CSNK2A1) , P19784 (CSNK2A2)
S253 Phosphorylation
S254 Phosphorylation
S289 Phosphorylation P68400 (CSNK2A1) , P19784 (CSNK2A2)

研究背景

功能:

B-lymphocyte-specific membrane protein that plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes. Functions as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR.

翻译修饰:

Phosphorylated on serines and threonines in resting B-cells. Protein kinase C/PKC can use CD20 as substrate.

细胞定位:

Cell membrane>Multi-pass membrane protein. Cell membrane>Lipid-anchor.
Note: Constitutively associated with membrane rafts.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed on B-cells.

亚基结构:

Forms homotetramers. Interacts with the heavy and light chains of cell surface IgM, the antigen-binding components of the BCR.

蛋白家族:

Belongs to the MS4A family.

研究领域

· Organismal Systems > Immune system > Hematopoietic cell lineage.   (View pathway)

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