CD20 Antibody - #BF0233

产品: CD20 抗体
货号: BF0233
描述: Mouse monoclonal antibody to CD20
应用: IHC IF/ICC ELISA
反应: Human
分子量: 33kDa.; 33kD(Calculated).
蛋白号: P11836
RRID: AB_2833614

浏览相似产品>>

   规格 价格 库存
 50ul RMB¥ 1800 现货
 100ul RMB¥ 2800 现货

货期: 当天发货

联系销售
相关下载
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Mouse
应用:
ELISA 1:10000, IHC 1:200-1:1000, IF/ICC 1:200-1:1000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human
克隆:
Monoclonal [AFB1589]
特异性:
CD20 antibody detects endogenous levels of total CD20.
RRID:
AB_2833614
引用格式: Affinity Biosciences Cat# BF0233, RRID:AB_2833614.
偶联:
Unconjugated.
纯化:
Affinity-chromatography.
保存:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

APY; ATOPY; B lymphocyte antigen CD20; B Lymphocyte Cell Surface Antigen B1; B-lymphocyte antigen CD20; B-lymphocyte cell-surface antigen B1; B-lymphocyte surface antigen B1; B1; Bp 35; Bp35; CD 20; CD20; CD20 antigen; CD20 receptor; CD20_HUMAN; CVID5; Fc epsilon receptor I beta chain; Fc Fragment of IgE high affinity I receptor for beta polypeptide; FCER1B; High affinity immunoglobulin epsilon receptor subunit beta; IgE Fc receptor subunit beta; IGEL; IGER; IGHER; LEU 16; LEU16; leukocyte surface antigen Leu 16; Leukocyte surface antigen Leu-16; Ly44; Membrane spanning 4 domains A1; Membrane spanning 4 domains subfamily A member 2; Membrane-spanning 4-domains subfamily A member 1; MS4A1; MS4A2; S7;

抗原和靶标

免疫原:

Purified recombinant fragment of human CD20 expressed in E. Coli.

Uniprot:

P11836(CD20_HUMAN) >>Visit HPA database.

基因/基因ID:
MS4A1(931)
表达:
P11836 CD20_HUMAN:

Expressed on B-cells.

描述:
CD20 (MS4A1, membrane-spanning 4-domains, subfamily A, member 1) is a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. The CD20 antigen is present on human pre B lymphocytes and on B lymphocytes at all stages of maturation, except on plasma cells. Low level expression of the CD20 antigen has been detected on normal T lymphocytes. The CD20 molecule is involved in regulation of B cell differentiation, presumably via its reported function as a Ca++ channel subunit. And it is known to accelerate the G0 to G1 progression induced by IGF-1.
序列:
MTTPRNSVNGTFPAEPMKGPIAMQSGPKPLFRRMSSLVGPTQSFFMRESKTLGAVQIMNGLFHIALGGLLMIPAGIYAPICVTVWYPLWGGIMYIISGSLLAATEKNSRKCLVKGKMIMNSLSLFAAISGMILSIMDILNIKISHFLKMESLNFIRAHTPYINIYNCEPANPSEKNSPSTQYCYSIQSLFLGILSVMLIFAFFQELVIAGIVENEWKRTCSRPKSNIVLLSAEEKKEQTIEIKEEVVGLTETSSQPKNEEDIEIIPIQEEEEEETETNFPEPPQDQESSPIENDSSP

翻译修饰 - P11836 作为底物

Site PTM Type Enzyme
T2 Phosphorylation
T3 Phosphorylation
S7 Phosphorylation
T11 Phosphorylation
S25 Phosphorylation
S35 Phosphorylation
S36 Phosphorylation
T41 Phosphorylation
S121 Phosphorylation
S225 Phosphorylation
S231 Phosphorylation P68400 (CSNK2A1) , P19784 (CSNK2A2)
K235 Ubiquitination
K236 Ubiquitination
K243 Ubiquitination
T250 Phosphorylation P68400 (CSNK2A1) , P19784 (CSNK2A2)
S253 Phosphorylation
S254 Phosphorylation
S289 Phosphorylation P68400 (CSNK2A1) , P19784 (CSNK2A2)

研究背景

功能:

B-lymphocyte-specific membrane protein that plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes. Functions as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR.

翻译修饰:

Phosphorylated on serines and threonines in resting B-cells. Protein kinase C/PKC can use CD20 as substrate.

细胞定位:

Cell membrane>Multi-pass membrane protein. Cell membrane>Lipid-anchor.
Note: Constitutively associated with membrane rafts.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed on B-cells.

亚基结构:

Forms homotetramers. Interacts with the heavy and light chains of cell surface IgM, the antigen-binding components of the BCR.

蛋白家族:

Belongs to the MS4A family.

研究领域

· Organismal Systems > Immune system > Hematopoietic cell lineage.   (View pathway)

限制条款

产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）

产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。

产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。

Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。

产品仅供科学研究使用。不用于诊断和治疗。 

产品未经授权不得转售。

Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.