RGS12 Antibody - #DF4415

Figure 1 - RGS12 is highly expressed in MIRI mice and H/R cells. (A) The MIRI model was developed using a 30 min ischemia and 24 h reperfusion. (B) Echocardiogram, LVEF and LVFS of mice. (C) Quantification of the infarct area in the myocardial tissue and images of infarct tissue. (D) Serum LDH and CK activity. (E) ROS levels (magnification, ×400) and (F) Fe2+ content in the myocardial tissue. RGS12 expression levels in the myocardial tissue was analyzed using (G) reverse-transcription quantitative PCR and western blotting and (H) immunofluorescence. Magnification, ×400. n=6. (I) A cell model of H/R-induced injury was developed using a 12 h serum starvation, 8 h hypoxia and 12 h reoxygenation. RGS12 expression levels in H/R cells was analyzed using (J) reverse transcription quantitative PCR and western blotting and (K) immunofluorescence. Magnification, ×400. n=3. Data were presented as the mean ± SD. MIRI, myocardial ischemia/reperfusion injury; H/R, hypoxia/reoxygenation; RGS12, regulator of G-protein signaling 12; LVEF, left ventricular ejection fraction; LVFS, left ventricular fractional shortening; LDH, lactate dehydrogenase; CK, creatine kinase; ROS, reactive oxygen species.