RGS10 Antibody - #DF4414

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产品: RGS10 抗体
货号: DF4414
描述: Rabbit polyclonal antibody to RGS10
应用: WB IHC IF/ICC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
蛋白号: O43665
RRID: AB_2836769

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
RGS10 Antibody detects endogenous levels of total RGS10.
RRID:
AB_2836769
引用格式: Affinity Biosciences Cat# DF4414, RRID:AB_2836769.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Regulator of G protein signaling 10; Regulator of G-protein signaling 10; RGS10; RGS10_HUMAN;

抗原和靶标

免疫原:

A synthesized peptide derived from human RGS10, corresponding to a region within the internal amino acids.

基因/基因ID:
RGS10(6001)

文献引用

1). Microglia Exhibit Distinct Heterogeneity Rather than M1/M2 Polarization within the Early Stage of Acute Ischemic Stroke. Aging and disease, 2023 (PubMed: 37199734) [IF=7.0]
2). miR‑199b‑5p mediates adriamycin‑induced podocyte apoptosis by inhibiting the expression of RGS10. Experimental and Therapeutic Medicine, 2021 (PubMed: 34737809) [IF=2.4]

Application: WB    Species: Mouse    Sample: podocytes

Figure 3 miR-199b-5p regulates the expression of RGS10. (A) Predicted binding sites of miR-199b-5p on the 3'UTR of RGS10. (B) RGS10 expression level after transient transfection and treatment of podocytes with miR-199b-5p mimic, NC mimic, ADR, ADR+miR-199b-5p mimic or ADR+NC mimic. (C) RGS10 expression level after the transient transfection and treatment of podocytes with miR-199b-5p inhibitor, NC inhibitor, ADR, ADR+miR-199b-5p inhibitor or ADR+NC inhibitor. (D) RGS10 protein expression in podocytes treated as in (B). (E) RGS10 protein expression in podocytes treated as in (C). (F) Expression level of RGS10 was determined by RT-qPCR in ADR-treated podocytes transfected with miR-199b-5p mimic or miR-199b-5p mimic+AdRGS10. (G) Expression of Bax and Bcl-2 was determined by western blotting in ADR-treated podocytes transfected as in (F). Control indicates normal podocytes. n=3 in each group. *P

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