G3BP2 Antibody - #DF4387

Fig. 5 Lnc9456 interacts with G3BP2 and promotes the nuclear translocation of p65 subunit of NF-κB. a The top 7 proteins that may bind to lnc9456 were predicted by catRAPID omics module. b The top 20 of Gene Ontology (GO) analysis of interacting proteins pulled down by biotinylated lnc9456 probe and identified by mass spectrometry. c Representative western blotting (upper) and its quantification (lower) of G3BP2 in myocardial tissues derived from mice at the indicated time post-LADL (n = 4). d Representative western blotting (upper) and its quantification (lower) of G3BP2 in HL-1 mouse cardiomyocytes transfected with smart silencer RNA of lnc9456 (ssRNA-lnc9456) or its negative control (ssRNA-NC) for 72 h (n = 4). e Representative western blotting (left) and its quantification (right) of G3BP2 in HL-1 mouse cardiomyocytes transfected with control plasmid (OE-NC) or lnc9456 overexpression plasmid (OE-lnc9456) for 48 h (n = 4). f RNA pull-down and western blotting detection for the binding of G3BP2 to lnc9456 in HL-1 mouse cardiomyocytes treated as mentioned in e. g Co-IP detection for the binding of G3BP2 to IκBα in HL-1 mouse cardiomyocytes treated as mentioned in e. h Immunofluorescence staining for cellular localization of NF-κB p65 (green) in HL-1 mouse cardiomyocytes treated as mentioned in e (n = 3). Data are presented as the means ± SEM. *P