PDHK1 Antibody - #DF4365

Fig. 3. Pimozide inhibits PKM2 protein and mRNA in both MCF-7 and MDA-MB-231 cells in vitro. (A-B) Cells were treated with the indicated concentrations of Pimozide for 24 h, and the protein expression of glycolytic enzymes in MCF-7(A) and MDA-MB-231(B) cells were determined by Western blot analysis (left panel). Densitometry analysis was performed to assess the glycolytic enzymes protein expression (normalized to β-actin expression), PKM2 decreased significantly compared with untreated cells (right panel). (C-D) The mRNA expression of PKM2 in MCF-7(C) and MDA-MB-231(D) cells untreated or treated with Pimozide was determined by qRT-PCR. GAPDH was used as a control. Data represent mean ± SD from three biological replicates (*p < 0.05, **p < 0.01).

Figure 5 SPC upregulate the protein expression of HIF-1a and PDK-1. (A) Western blot image of HIF-1a protein. (B) Western blot image of PDK-1 protein. (C) HIF-1a protein expression. (D) PDK-1 protein expression. Protein content was normalized to GAPDH. Data represent mean ± SD (n = 3/group). *P < 0.05 vs Con, #P < 0.05 vs H/R, &P < 0.05 vs SPC.