产品: ZIC1/2/3 抗体
货号: AF0340
描述: Rabbit polyclonal antibody to ZIC1/2/3
应用: WB IHC IF/ICC
反应: Human, Mouse
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Chicken, Xenopus
分子量: 51kDa; 48kD,55kD,51kD(Calculated).
蛋白号: Q15915 | O95409 | O60481
RRID: AB_2833294

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
预测:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Chicken(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
ZIC1/2/3 Antibody detects endogenous levels of total ZIC1/2/3.
RRID:
AB_2833294
引用格式: Affinity Biosciences Cat# AF0340, RRID:AB_2833294.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Odd paired homolog Drosophila; Zic 1; ZIC; Zic family member 1 (odd-paired Drosophila homolog); Zic family member 1; Zic protein member 1; zic1; ZIC1_HUMAN; Zinc finger protein 201; Zinc finger protein of the cerebellum 1; Zinc finger protein ZIC 1; Zinc finger protein ZIC1; ZNF 201; ZNF201; HPE 5; HPE5; Odd paired homolog Drosophila; Zic 2; Zic family member 2 (odd paired Drosophila homolog); Zic family member 2; ZIC2; ZIC2_HUMAN; Zinc finger protein of the cerebellum 2; Zinc finger protein ZIC 2; Zinc finger protein Zic2; Heterotaxy 1; HTX; HTX1; ZIC 3; Zic family member 3 (odd paired Drosophila homolog heterotaxy 1); Zic family member 3; Zic3; ZIC3_HUMAN; Zinc finger protein 203; Zinc finger protein of the cerebellum 3; Zinc finger protein ZIC 3 (Zinc finger protein of the cerebellum 3); Zinc finger protein ZIC 3; ZNF203;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
Q15915 ZIC1_HUMAN:

CNS. A high level expression is seen in the cerebellum. Detected in the nuclei of the cerebellar granule cell lineage from the progenitor cells of the external germinal layer to the postmigrated cells of the internal granular layer. Detected in medulloblastoma (26/29 cases), but not present in all other tumors examined.

描述:
Q15915; ZIC; Zic family member 1 (odd-paired Drosophila homolog); Zic family member 1 (odd-paired homolog, Drosophila); ZIC1; zinc finger protein of the cerebellum 1; zinc finger protein ZIC 1; ZNF201
序列:
MLLDAGPQYPAIGVTTFGASRHHSAGDVAERDVGLGINPFADGMGAFKLNPSSHELASAGQTAFTSQAPGYAAAAALGHHHHPGHVGSYSSAAFNSTRDFLFRNRGFGDAAAAASAQHSLFAASAGGFGGPHGHTDAAGHLLFPGLHEQAAGHASPNVVNGQMRLGFSGDMYPRPEQYGQVTSPRSEHYAAPQLHGYGPMNVNMAAHHGAGAFFRYMRQPIKQELICKWIEPEQLANPKKSCNKTFSTMHELVTHVTVEHVGGPEQSNHICFWEECPREGKPFKAKYKLVNHIRVHTGEKPFPCPFPGCGKVFARSENLKIHKRTHTGEKPFKCEFEGCDRRFANSSDRKKHMHVHTSDKPYLCKMCDKSYTHPSSLRKHMKVHESSSQGSQPSPAASSGYESSTPPTIVSPSTDNPTTSSLSPSSSAVHHTAGHSALSSNFNEWYV

MLLDAGPQFPAIGVGSFARHHHHSAAAAAAAAAEMQDRELSLAAAQNGFVDSAAAHMGAFKLNPGAHELSPGQSSAFTSQGPGAYPGSAAAAAAAAALGPHAAHVGSYSGPPFNSTRDFLFRSRGFGDSAPGGGQHGLFGPGAGGLHHAHSDAQGHLLFPGLPEQHGPHGSQNVLNGQMRLGLPGEVFGRSEQYRQVASPRTDPYSAAQLHNQYGPMNMNMGMNMAAAAAHHHHHHHHHPGAFFRYMRQQCIKQELICKWIDPEQLSNPKKSCNKTFSTMHELVTHVSVEHVGGPEQSNHVCFWEECPREGKPFKAKYKLVNHIRVHTGEKPFPCPFPGCGKVFARSENLKIHKRTHTGEKPFQCEFEGCDRRFANSSDRKKHMHVHTSDKPYLCKMCDKSYTHPSSLRKHMKVHESSPQGSESSPAASSGYESSTPPGLVSPSAEPQSSSNLSPAAAAAAAAAAAAAAAVSAVHRGGGSGSGGAGGGSGGGSGSGGGGGGAGGGGGGSSGGGSGTAGGHSGLSSNFNEWYV

MTMLLDGGPQFPGLGVGSFGAPRHHEMPNREPAGMGLNPFGDSTHAAAAAAAAAAFKLSPAAAHDLSSGQSSAFTPQGSGYANALGHHHHHHHHHHHTSQVPSYGGAASAAFNSTREFLFRQRSSGLSEAASGGGQHGLFAGSASSLHAPAGIPEPPSYLLFPGLHEQGAGHPSPTGHVDNNQVHLGLRGELFGRADPYRPVASPRTDPYAAGAQFPNYSPMNMNMGVNVAAHHGPGAFFRYMRQPIKQELSCKWIDEAQLSRPKKSCDRTFSTMHELVTHVTMEHVGGPEQNNHVCYWEECPREGKSFKAKYKLVNHIRVHTGEKPFPCPFPGCGKIFARSENLKIHKRTHTGEKPFKCEFEGCDRRFANSSDRKKHMHVHTSDKPYICKVCDKSYTHPSSLRKHMKVHESQGSDSSPAASSGYESSTPPAIASANSKDTTKTPSAVQTSTSHNPGLPPNFNEWYV

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Xenopus
100
Zebrafish
100
Chicken
100
Dog
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q15915/O95409/O60481 作为底物

Site PTM Type Enzyme
Y9 Phosphorylation
T15 Phosphorylation
S20 Phosphorylation
T245 Phosphorylation
S247 Phosphorylation
K311 Acetylation
T325 Phosphorylation
T327 Phosphorylation
Site PTM Type Enzyme
S24 Phosphorylation
S199 Phosphorylation
K253 Sumoylation
K253 Ubiquitination
S267 Phosphorylation
T276 Phosphorylation
S278 Phosphorylation
T328 Phosphorylation
K342 Acetylation
T356 Phosphorylation
T358 Phosphorylation
Site PTM Type Enzyme
S18 Phosphorylation
S59 Phosphorylation
K248 Sumoylation
T351 Phosphorylation
T353 Phosphorylation

研究背景

功能:

Acts as a transcriptional activator. Involved in neurogenesis. Plays important roles in the early stage of organogenesis of the CNS, as well as during dorsal spinal cord development and maturation of the cerebellum. Involved in the spatial distribution of mossy fiber (MF) neurons within the pontine gray nucleus (PGN). Plays a role in the regulation of MF axon pathway choice. Promotes MF migration towards ipsilaterally-located cerebellar territories. May have a role in shear flow mechanotransduction in osteocytes. Retains nuclear GLI1 and GLI3 in the cytoplasm. Binds to the minimal GLI-consensus sequence 5'-TGGGTGGTC-3' (By similarity).

细胞定位:

Nucleus. Cytoplasm.
Note: Localizes in the cytoplasm in presence of MDFIC overexpression.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

CNS. A high level expression is seen in the cerebellum. Detected in the nuclei of the cerebellar granule cell lineage from the progenitor cells of the external germinal layer to the postmigrated cells of the internal granular layer. Detected in medulloblastoma (26/29 cases), but not present in all other tumors examined.

亚基结构:

Interacts (via the C2H2-type domains 3, 4 and 5) with MDFIC (via the C2H2-type domains 3, 4 and 5). Interacts with GLI1; the interaction enhances transcription activation. Interacts with GLI2. Interacts with GLI3; the interaction enhances transcription activation (By similarity).

蛋白家族:

The C2H2-type 3, 4 and 5 zinc finger domains are necessary for transcription activation.

Belongs to the GLI C2H2-type zinc-finger protein family.

功能:

Acts as a transcriptional activator or repressor. Plays important roles in the early stage of organogenesis of the CNS. Activates the transcription of the serotonin transporter SERT in uncrossed ipsilateral retinal ganglion cells (iRGCs) to refine eye-specific projections in primary visual targets. Its transcriptional activity is repressed by MDFIC. Involved in the formation of the ipsilateral retinal projection at the optic chiasm midline. Drives the expression of EPHB1 on ipsilaterally projecting growth cones. Binds to the minimal GLI-consensus sequence 5'-TGGGTGGTC-3'. Associates to the basal SERT promoter region from ventrotemporal retinal segments of retinal embryos.

翻译修饰:

Phosphorylated.

Ubiquitinated by RNF180, leading to its degradation.

细胞定位:

Nucleus. Cytoplasm.
Note: Localizes in the cytoplasm in presence of MDFIC overexpression. Both phosphorylated and unphosphorylated forms are localized in the nucleus (By similarity).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Interacts with RNF180. Interacts (via the C2H2-type domains 3, 4 and 5) with MDFIC (via the C2H2-type domains 3, 4 and 5); the interaction reduces its transcriptional activity. Interacts with GLI1 and GLI2 (By similarity). Interacts (via C2H2-type domain 3) with DHX9.

蛋白家族:

The C2H2-type 3, 4 and 5 zinc finger domains are necessary for transcription activation.

Belongs to the GLI C2H2-type zinc-finger protein family.

功能:

Acts as transcriptional activator. Required in the earliest stages in both axial midline development and left-right (LR) asymmetry specification. Binds to the minimal GLI-consensus sequence 5'-GGGTGGTC-3'.

细胞定位:

Nucleus. Cytoplasm.
Note: Localizes in the cytoplasm in presence of MDFIC overexpression (By similarity). Translocation to the nucleus requires KPNA1 or KPNA6.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Interacts (via the C2H2-type domains 3, 4 and 5) with MDFIC (via the C2H2-type domains 3, 4 and 5); the interaction reduces its transcriptional activity (By similarity). Interacts with KPNA1 and KPNA6. Interacts (via C2H2-type domains 3, 4 and 5) with GLI3; the interaction enhances its transcriptional activity.

蛋白家族:

The C2H2-type 3, 4 and 5 zinc finger domains are necessary for transcription activation.

Belongs to the GLI C2H2-type zinc-finger protein family.

研究领域

· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells.   (View pathway)

文献引用

1). Wei Yi et al. The expression status of ZIC2 as a prognostic marker for nasopharyngeal carcinoma. International Journal of Clinical and Experimental Pathology 2018;11(9):4446-4460 (PubMed: 31949842) [IF=1.4]

Application: IHC    Species: Human    Sample: NPC tissues

Figure 2 Expression analysis of ZIC2 protein as determined by immunohistochemistry (200×). ZIC2 expression was localized in the nuclei and cytoplasms of NPC cells. A. Negative staining of ZIC2 in NPC tissues. B. “+” (score 1-4, weakly positive) expression of ZIC2 in NPC tissues. C. “++” (score 5-8, positive) expression of ZIC2 in NPC tissues. D. “+++” (score 9-12, strongly positive) expression of ZIC2 in NPC tissues.

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