| 产品: | RAB11FIP2 抗体 |
| 货号: | DF4370 |
| 描述: | Rabbit polyclonal antibody to RAB11FIP2 |
| 应用: | WB IF/ICC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 58 KD; 58kD(Calculated). |
| 蛋白号: | Q7L804 |
| RRID: | AB_2836658 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF4370, RRID:AB_2836658.
展开/折叠
KIAA0941; NRip11; RAB11 family interacting protein 2 (class I); Rab11 family-interacting protein 2; RAB11 FIP2 long isoform; Rab11-FIP2; RAB11FIP2; RFIP2; RFIP2_HUMAN;
抗原和靶标
- Q7L804 RFIP2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MMLSEQAQKWFPTHVQVTVLQAKDLKPKGKSGTNDTYTIIQLGKEKYSTSVAEKTLEPVWKEEASFELPGLLIQGSPEKYILFLIVMHRSLVGLDKFLGQVAINLNDIFEDKQRRKTEWFRLESKQGKRIKNRGEIKVNIQFMRNNMTASMFDLSMKDKTRSPFAKLKDKMKGRKNDGTFSDTSSAIIPSTHMPDANSEFSSGEIQMKSKPKKPFLLGPQRLSSAHSMSDLSGSHMSSEKLKAGTIGQTHLLGHQLDSFGTVPESGSLKSPHRRTLSFDTSKMNQPDSIVDEGELCFGRQNDPFTNVTASLPQKFATLPRKKNPFEESSETWDSSMNLFSKPIEIRKENKREKREKVSLFERVTGKKDSRRSDKLNNGGSDSPCDLKSPNAFSENRQDYFDYESTNPFTAKFRASNIMPSSSFHMSPTSNEDLRKIPDSNPFDATAGYRSLTYEEVLQELVKHKELLRRKDTHIRELEDYIDNLLVRVMEETPSILRVPYEPSRKAGKFSNS
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q7L804 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T13 | Phosphorylation | Uniprot | |
| K46 | Ubiquitination | Uniprot | |
| T148 | Phosphorylation | Uniprot | |
| S150 | Phosphorylation | Uniprot | |
| S155 | Phosphorylation | Uniprot | |
| S223 | Phosphorylation | Uniprot | |
| S227 | Phosphorylation | Q7KZI7 (MARK2) | Uniprot |
| S229 | Phosphorylation | Uniprot | |
| T245 | Phosphorylation | Uniprot | |
| S258 | Phosphorylation | Uniprot | |
| T261 | Phosphorylation | Uniprot | |
| T275 | Phosphorylation | Uniprot | |
| S277 | Phosphorylation | Uniprot | |
| T280 | Phosphorylation | Uniprot | |
| S288 | Phosphorylation | Uniprot | |
| T317 | Phosphorylation | Uniprot | |
| K322 | Ubiquitination | Uniprot | |
| K341 | Ubiquitination | Uniprot | |
| K350 | Acetylation | Uniprot | |
| S382 | Phosphorylation | Uniprot | |
| K387 | Methylation | Uniprot | |
| S388 | Phosphorylation | Uniprot | |
| Y399 | Phosphorylation | Uniprot | |
| S429 | Phosphorylation | Uniprot | |
| Y480 | Phosphorylation | Uniprot | |
| S494 | Phosphorylation | Uniprot | |
| Y500 | Phosphorylation | Uniprot |
研究背景
A Rab11 effector binding preferentially phosphatidylinositol 3,4,5-trisphosphate (PtdInsP3) and phosphatidic acid (PA) and acting in the regulation of the transport of vesicles from the endosomal recycling compartment (ERC) to the plasma membrane. Involved in insulin granule exocytosis. Also involved in receptor-mediated endocytosis and membrane trafficking of recycling endosomes, probably originating from clathrin-coated vesicles. Required in a complex with MYO5B and RAB11 for the transport of NPC1L1 to the plasma membrane. Also acts as a regulator of cell polarity. Plays an essential role in phagocytosis through a mechanism involving TICAM2, RAC1 and CDC42 Rho GTPases for controlling actin-dynamics.
Phosphorylation at Ser-227 by MARK2 regulates epithelial cell polarity.
Cell projection>Phagocytic cup. Cell membrane>Peripheral membrane protein. Recycling endosome membrane>Peripheral membrane protein.
Note: Translocates with RAB11A from the vesicles of the endocytic recycling compartment (ERC) to the plasma membrane.
Homooligomerizes in a Rab11-independent manner. Forms a heterooligomeric complex with RAB11FIP4. Interacts with AP2A1, MYO5B, RAB25 and REPS1. Interacts with RAB11A and RAB11B (activated GTP-bound form). Interacts with NPC1L1. Interacts (via NPF motifs) with EHD1 and EHD3. Interacts with TICAM2; this interaction directs RAB11FIP2 to the phagosome.
研究领域
· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)
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