| 产品: | MLF1 抗体 |
| 货号: | DF4192 |
| 描述: | Rabbit polyclonal antibody to MLF1 |
| 应用: | WB IF/ICC |
| 反应: | Human, Mouse |
| 预测: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken |
| 分子量: | 30 KD; 31kD(Calculated). |
| 蛋白号: | P58340 |
| RRID: | AB_2836557 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF4192, RRID:AB_2836557.
展开/折叠
Hls7; MLF1; MLF1_HUMAN; Myelodysplasia myeloid leukemia factor 1; Myelodysplasia-myeloid leukemia factor 1; Myeloid leukemia factor 1; myeloid leukemia factor 1 variant 1; myeloid leukemia factor 1 variant 2; myeloid leukemia factor 1 variant 3;
抗原和靶标
Most abundant in testis, ovary, skeletal muscle, heart, kidney and colon. Low expression in spleen, thymus and peripheral blood leukocytes.
- P58340 MLF1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MFRMLNSSFEDDPFFSESILAHRENMRQMIRSFSEPFGRDLLSISDGRGRAHNRRGHNDGEDSLTHTDVSSFQTMDQMVSNMRNYMQKLERNFGQLSVDPNGHSFCSSSVMTYSKIGDEPPKVFQASTQTRRAPGGIKETRKAMRDSDSGLEKMAIGHHIHDRAHVIKKSKNKKTGDEEVNQEFINMNESDAHAFDEEWQSEVLKYKPGRHNLGNTRMRSVGHENPGSRELKRREKPQQSPAIEHGRRSNVLGDKLHIKGSSVKSNKK
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P58340 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S8 | Phosphorylation | Uniprot | |
| R23 | Methylation | Uniprot | |
| R27 | Methylation | Uniprot | |
| S32 | Phosphorylation | Uniprot | |
| S34 | Phosphorylation | Uniprot | |
| R48 | Methylation | Uniprot | |
| R50 | Methylation | Uniprot | |
| Y85 | Phosphorylation | Uniprot | |
| K88 | Ubiquitination | Uniprot | |
| T112 | Phosphorylation | Uniprot | |
| Y113 | Phosphorylation | Uniprot | |
| K122 | Ubiquitination | Uniprot | |
| K168 | Acetylation | Uniprot | |
| K169 | Acetylation | Uniprot | |
| K174 | Ubiquitination | Uniprot | |
| Y206 | Phosphorylation | Uniprot | |
| R219 | Methylation | Uniprot | |
| S220 | Phosphorylation | Uniprot | |
| K236 | Ubiquitination | Uniprot |
研究背景
Involved in lineage commitment of primary hemopoietic progenitors by restricting erythroid formation and enhancing myeloid formation. Interferes with erythropoietin-induced erythroid terminal differentiation by preventing cells from exiting the cell cycle through suppression of CDKN1B/p27Kip1 levels. Suppresses COP1 activity via CSN3 which activates p53 and induces cell cycle arrest. Binds DNA and affects the expression of a number of genes so may function as a transcription factor in the nucleus.
Phosphorylation is required for binding to YWHAZ.
Cytoplasm. Nucleus. Cell projection>Cilium. Cytoplasm>Cytoskeleton>Cilium basal body.
Note: Shuttles between the cytoplasm and nucleus.
Most abundant in testis, ovary, skeletal muscle, heart, kidney and colon. Low expression in spleen, thymus and peripheral blood leukocytes.
Interacts with CENPU. Also interacts with NRBP1/MADM, YWHAZ/14-3-3-zeta and HNRPUL2/MANP. NRBP1 recruits a serine kinase which phosphorylates both itself and MLF1. Phosphorylated MLF1 then binds to YWHAZ and is retained in the cytoplasm. Retained in the nucleus by binding to HNRPUL2. Binds to COPS3/CSN3 which is required for suppression of COP1 and activation of p53.
Belongs to the MLF family.
研究领域
· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.
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