产品: PTK6 抗体
货号: BF0203
描述: Mouse monoclonal antibody to PTK6
应用: WB ELISA
反应: Human
分子量: 52kDa; 52kD(Calculated).
蛋白号: Q13882
RRID: AB_2833592

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产品描述

来源:
Mouse
应用:
ELISA 1:10000, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human
克隆:
Monoclonal [AFB1567]
特异性:
PTK6 antibody detects endogenous levels of total PTK6.
RRID:
AB_2833592
引用格式: Affinity Biosciences Cat# BF0203, RRID:AB_2833592.
偶联:
Unconjugated.
纯化:
Affinity-chromatography.
保存:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Breast tumor kinase; Breast tumour kinase; Protein tyrosine kinase 6; Protein-tyrosine kinase 6; Ptk6; PTK6_HUMAN; Tyrosine protein kinase BRK; Tyrosine-protein kinase BRK;

抗原和靶标

免疫原:

Purified recombinant fragment of human PTK6 expressed in E. Coli.

Uniprot:
基因/基因ID:
表达:
Q13882 PTK6_HUMAN:

Epithelia-specific. Very high level in colon and high levels in small intestine and prostate, and low levels in some fetal tissues. Not expressed in breast or ovarian tissue but expressed in high percentage of breast and ovarian cancers. Also overexpressed in some metastatic melanomas, lymphomas, colon cancers, squamous cell carcinomas and prostate cancers. Also found in melanocytes. Not expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Isoform 2 is present in prostate epithelial cell lines derived from normal prostate and prostate adenocarcinomas, as well as in a variety of cell lines.

描述:
The protein encoded by this gene is a cytoplasmic nonreceptor protein kinase which may function as an intracellular signal transducer in epithelial tissues. Overexpression of this gene in mammary epithelial cells leads to sensitization of the cells to epidermal growth factor and results in a partially transformed phenotype. Expression of this gene has been detected at low levels in some breast tumors but not in normal breast tissue. The encoded protein has been shown to undergo autophosphorylation.
序列:
MVSRDQAHLGPKYVGLWDFKSRTDEELSFRAGDVFHVARKEEQWWWATLLDEAGGAVAQGYVPHNYLAERETVESEPWFFGCISRSEAVRRLQAEGNATGAFLIRVSEKPSADYVLSVRDTQAVRHYKIWRRAGGRLHLNEAVSFLSLPELVNYHRAQSLSHGLRLAAPCRKHEPEPLPHWDDWERPREEFTLCRKLGSGYFGEVFEGLWKDRVQVAIKVISRDNLLHQQMLQSEIQAMKKLRHKHILALYAVVSVGDPVYIITELMAKGSLLELLRDSDEKVLPVSELLDIAWQVAEGMCYLESQNYIHRDLAARNILVGENTLCKVGDFGLARLIKEDVYLSHDHNIPYKWTAPEALSRGHYSTKSDVWSFGILLHEMFSRGQVPYPGMSNHEAFLRVDAGYRMPCPLECPPSVHKLMLTCWCRDPEQRPCFKALRERLSSFTSYENPT

翻译修饰 - Q13882 作为底物

Site PTM Type Enzyme
Y13 Phosphorylation Q13882 (PTK6)
Y61 Phosphorylation Q13882 (PTK6)
Y66 Phosphorylation Q13882 (PTK6)
S111 Phosphorylation
Y114 Phosphorylation Q13882 (PTK6)
S117 Phosphorylation
Y127 Phosphorylation
S159 Phosphorylation
S279 Phosphorylation
Y342 Phosphorylation Q13882 (PTK6)
S344 Phosphorylation
Y351 Phosphorylation Q13882 (PTK6)
K352 Ubiquitination
T445 Phosphorylation
S446 Phosphorylation
Y447 Phosphorylation Q13882 (PTK6) , Q9H3Y6 (SRMS)

翻译修饰 - Q13882 作为激酶

Substrate Site Source
P00533 (EGFR) Y869 Uniprot
P31749 (AKT1) Y315 Uniprot
P31749 (AKT1) Y326 Uniprot
P35222 (CTNNB1) Y64 Uniprot
P35222 (CTNNB1) Y142 Uniprot
P35222 (CTNNB1) Y331 Uniprot
P35222 (CTNNB1) Y333 Uniprot
P40763 (STAT3) Y705 Uniprot
P42229 (STAT5A) Y694 Uniprot
P49023-2 (PXN) Y31 Uniprot
P49023-2 (PXN) Y118 Uniprot
P51692 (STAT5B) Y699 Uniprot
P56945 (BCAR1) Y165 Uniprot
P56945 (BCAR1) Y224 Uniprot
P56945 (BCAR1) Y234 Uniprot
P56945 (BCAR1) Y249 Uniprot
P56945 (BCAR1) Y262 Uniprot
P56945 (BCAR1) Y267 Uniprot
P56945 (BCAR1) Y287 Uniprot
P56945 (BCAR1) Y306 Uniprot
P56945 (BCAR1) Y327 Uniprot
P56945 (BCAR1) Y362 Uniprot
P56945 (BCAR1) Y387 Uniprot
P56945 (BCAR1) Y664 Uniprot
Q05397 (PTK2) Y861 Uniprot
Q07666 (KHDRBS1) Y435 Uniprot
Q07666 (KHDRBS1) Y440 Uniprot
Q07666-1 (KHDRBS1) Y443 Uniprot
Q13017 (ARHGAP5) Y1109 Uniprot
Q13882 (PTK6) Y13 Uniprot
Q13882 (PTK6) Y61 Uniprot
Q13882 (PTK6) Y66 Uniprot
Q13882 (PTK6) Y114 Uniprot
Q13882-1 (PTK6) Y342 Uniprot
Q13882 (PTK6) Y351 Uniprot
Q13882 (PTK6) Y447 Uniprot
Q96P48 (ARAP1) Y231 Uniprot
Q99704 (DOK1) Y362 Uniprot
Q9NRY4 (ARHGAP35) Y1105 Uniprot
Q9UGK3 (STAP2) Y250 Uniprot

研究背景

功能:

Non-receptor tyrosine-protein kinase implicated in the regulation of a variety of signaling pathways that control the differentiation and maintenance of normal epithelia, as well as tumor growth. Function seems to be context dependent and differ depending on cell type, as well as its intracellular localization. A number of potential nuclear and cytoplasmic substrates have been identified. These include the RNA-binding proteins: KHDRBS1/SAM68, KHDRBS2/SLM1, KHDRBS3/SLM2 and SFPQ/PSF; transcription factors: STAT3 and STAT5A/B and a variety of signaling molecules: ARHGAP35/p190RhoGAP, PXN/paxillin, BTK/ATK, STAP2/BKS. Associates also with a variety of proteins that are likely upstream of PTK6 in various signaling pathways, or for which PTK6 may play an adapter-like role. These proteins include ADAM15, EGFR, ERBB2, ERBB3 and IRS4. In normal or non-tumorigenic tissues, PTK6 promotes cellular differentiation and apoptosis. In tumors PTK6 contributes to cancer progression by sensitizing cells to mitogenic signals and enhancing proliferation, anchorage-independent survival and migration/invasion. Association with EGFR, ERBB2, ERBB3 may contribute to mammary tumor development and growth through enhancement of EGF-induced signaling via BTK/AKT and PI3 kinase. Contributes to migration and proliferation by contributing to EGF-mediated phosphorylation of ARHGAP35/p190RhoGAP, which promotes association with RASA1/p120RasGAP, inactivating RhoA while activating RAS. EGF stimulation resulted in phosphorylation of PNX/Paxillin by PTK6 and activation of RAC1 via CRK/CrKII, thereby promoting migration and invasion. PTK6 activates STAT3 and STAT5B to promote proliferation. Nuclear PTK6 may be important for regulating growth in normal epithelia, while cytoplasmic PTK6 might activate oncogenic signaling pathways.

Isoform 2 inhibits PTK6 phosphorylation and PTK6 association with other tyrosine-phosphorylated proteins.

翻译修饰:

Autophosphorylated. Autophosphorylation of Tyr-342 leads to an increase of kinase activity. Tyr-447 binds to the SH2 domain when phosphorylated and negatively regulates kinase activity.

细胞定位:

Cytoplasm. Nucleus. Cell projection>Ruffle. Membrane.
Note: Colocalizes with KHDRBS1, KHDRBS2 or KHDRBS3, within the nucleus. Nuclear localization in epithelial cells of normal prostate but cytoplasmic localization in cancer prostate.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Epithelia-specific. Very high level in colon and high levels in small intestine and prostate, and low levels in some fetal tissues. Not expressed in breast or ovarian tissue but expressed in high percentage of breast and ovarian cancers. Also overexpressed in some metastatic melanomas, lymphomas, colon cancers, squamous cell carcinomas and prostate cancers. Also found in melanocytes. Not expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Isoform 2 is present in prostate epithelial cell lines derived from normal prostate and prostate adenocarcinomas, as well as in a variety of cell lines.

亚基结构:

Interacts with GAP-A.p65 (By similarity). Interacts (via SH3 and SH2 domains) with KHDRBS1. Interacts (via SH3 and SH2 domains) with phosphorylated IRS4. Interacts with ADAM15. Interacts (via SH3 domain) with SFPQ. Interacts with EGFR and ERBB2. Interacts with STAP2. Interacts with PNX. Interacts with SFPQ. Interacts with PTK/ATK. Interacts with CTNNB1.

蛋白家族:

The SH3 domain plays a major role in substrate interactions. The SH2 domain of PTK6 plays a role in protein-protein interactions, but is likely more important for the regulation of catalytic activity.

Belongs to the protein kinase superfamily. Tyr protein kinase family. BRK/PTK6/SIK subfamily.

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