TOMM20 Antibody - #DF4179

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产品: TOMM20 抗体
货号: DF4179
描述: Rabbit polyclonal antibody to TOMM20
应用: WB IHC IF/ICC
文献验证: IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Rabbit, Dog
蛋白号: Q15388
RRID: AB_2836544

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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实验方案
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IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
TOMM20 Antibody detects endogenous levels of total TOMM20.
RRID:
AB_2836544
引用格式: Affinity Biosciences Cat# DF4179, RRID:AB_2836544.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

KIAA0016; MAS20; MGC117367; Mitochondrial 20 kDa outer membrane protein; Mitochondrial import receptor subunit TOM20 homolog; MOM19; Outer mitochondrial membrane receptor Tom20; TOM20; TOM20_HUMAN; TOMM20; Translocase of outer mitochondrial membrane 20 homolog (yeast); Translocase of outer mitochondrial membrane 20 homolog type II;

抗原和靶标

免疫原:

A synthesized peptide derived from human TOMM20, corresponding to a region within the internal amino acids.

基因/基因ID:
TOMM20(9804)

文献引用

1). Melatonin Promotes in vitro Development of Vitrified-Warmed Mouse GV Oocytes, Potentially by Modulating Phosphorylation of Drp1. Frontiers in veterinary science, 2021 (PubMed: 34631868) [IF=3.2]

Application: IF/ICC    Species: Mouse    Sample:

Figure 4. Effects of melatonin on mitochondrial mass and mitochondrial function in MII oocytes derived from vitrified-warmed mouse GV oocytes. (A) Representative images of TOMM20 in mouse MII oocytes. Scale bar, 50 μm. (B) The fluorescence intensity of TOMM20 was recorded using ImageJ software. In total, n = 197 MII oocytes were used in this assay (F: n = 53; V: n = 49; VM: n = 48; VMD: n = 47). (C) The expression levels of mitochondrial biogenesis related genes. (D) Representative images of mitochondrial membrane potential (MMP) in mouse MII oocytes. Scale bar, 50 μm. (E) The rate of mitochondrial membrane potential was recorded using ImageJ software. In total, n = 129 MII oocytes were used in this assay (F: n = 32; V: n = 27; VM: n = 36; VMD: n = 34). (F) The ATP content in different groups. In total, n = 120 MII oocytes were used in this assay (F: n = 30; V: n = 30; VM: n = 30; VMD: n = 30). Data are presented as mean ± SEM of three independent experiments. Groups without a common superscript are significantly different (P < 0.05). The four experimental groups: Fresh control (F), vitrification (V), vitrification + MT (VM), and vitrification + Mdivi-1 (VMD).

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