KCNN4 Antibody - #DF4132

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产品: KCNN4 抗体
货号: DF4132
描述: Rabbit polyclonal antibody to KCNN4
应用: WB IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Horse, Sheep, Rabbit, Dog
分子量: 48 KD; 48kD(Calculated).
蛋白号: O15554
RRID: AB_2836497

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Horse(91%), Sheep(100%), Rabbit(100%), Dog(100%)
克隆:
Polyclonal
特异性:
KCNN4 Antibody detects endogenous levels of total KCNN4.
RRID:
AB_2836497
引用格式: Affinity Biosciences Cat# DF4132, RRID:AB_2836497.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

DHS2; hIKCa1; hKCa4; hSK4; IK; IK1; IKCa1; Intermediate conductance calcium activated potassium channel protein 4; Intermediate conductance calcium-activated potassium channel protein 4; KCa3.1; KCa4; KCNN 4; Kcnn4; KCNN4_HUMAN; Potassium calcium activated channel subfamily N member 4; Potassium intermediate/small conductance calcium activated channel, subfamily N, member 4; Putative Gardos channel; SK4; SKCa 4; SKCa4;

抗原和靶标

免疫原:
Uniprot:

O15554(KCNN4_HUMAN) >>Visit HPA database.

基因/基因ID:
KCNN4(3783)
表达:
O15554 KCNN4_HUMAN:

Widely expressed in non-excitable tissues.

序列:
MGGDLVLGLGALRRRKRLLEQEKSLAGWALVLAGTGIGLMVLHAEMLWFGGCSWALYLFLVKCTISISTFLLLCLIVAFHAKEVQLFMTDNGLRDWRVALTGRQAAQIVLELVVCGLHPAPVRGPPCVQDLGAPLTSPQPWPGFLGQGEALLSLAMLLRLYLVPRAVLLRSGVLLNASYRSIGALNQVRFRHWFVAKLYMNTHPGRLLLGLTLGLWLTTAWVLSVAERQAVNATGHLSDTLWLIPITFLTIGYGDVVPGTMWGKIVCLCTGVMGVCCTALLVAVVARKLEFNKAEKHVHNFMMDIQYTKEMKESAARVLQEAWMFYKHTRRKESHAARRHQRKLLAAINAFRQVRLKHRKLREQVNSMVDISKMHMILYDLQQNLSSSHRALEKQIDTLAGKLDALTELLSTALGPRQLPEPSQQSK

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Sheep
100
Dog
100
Rabbit
100
Horse
91
Zebrafish
67
Bovine
0
Xenopus
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - O15554 作为底物

Site PTM Type Enzyme
S178 Phosphorylation
Y199 Phosphorylation
S314 Phosphorylation
T329 Phosphorylation
S334 Phosphorylation P17612 (PRKACA)
H358 Phosphorylation P22392 (NME2)
S367 Phosphorylation
K394 Ubiquitination
T398 Phosphorylation

研究背景

功能:

Forms a voltage-independent potassium channel that is activated by intracellular calcium. Activation is followed by membrane hyperpolarization which promotes calcium influx. Required for maximal calcium influx and proliferation during the reactivation of naive T-cells. Plays a role in the late stages of EGF-induced macropinocytosis.

翻译修饰:

Phosphorylation at His-358 by NDKB activates the channel, and conversely it's dephosphorylation by PHPT1 inhibits the channel.

细胞定位:

Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Widely expressed in non-excitable tissues.

亚基结构:

Heterotetramer of potassium channel proteins (Probable). Interacts with MTMR6.

蛋白家族:

Belongs to the potassium channel KCNN family. KCa3.1/KCNN4 subfamily.

研究领域

· Organismal Systems > Endocrine system > Insulin secretion.   (View pathway)

· Organismal Systems > Digestive system > Salivary secretion.

· Organismal Systems > Digestive system > Protein digestion and absorption.

文献引用

1). A prospective diagnostic and prognostic biomarker for hepatocellular carcinoma that functions in glucose metabolism regulation: Solute carrier family 37 member 3. Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease (PubMed: 36773462) [IF=6.2]
2). KCNN4 is a potential prognostic marker and critical factor affecting the immune status of the tumor microenvironment in kidney renal clear cell carcinoma. Translational Andrology and Urology (PubMed: 34295732) [IF=2.0]

Application: WB    Species: Human    Sample: kidney renal clear cell

Figure 13 Expression of KCNN4 increased in KIRC samples. (A) Expression of KCNN4 in KIRC samples and normal adjacent non-tumor controls were assessed by qPCR (*P<0.05). (B,C) Representative blots and quantification results of western blot showed that expression of the KCNN4 protein in KIRC was significantly higher than controls (*P<0.05). KIRC, kidney renal clear cell carcinoma.

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