产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF4088, RRID:AB_2836462.
展开/折叠
Glycine receptor 58 kDa subunit; Glycine receptor beta; Glycine receptor subunit beta; Glycine receptor, beta subunit; OTTHUMP00000220896; OTTHUMP00000220897;
抗原和靶标
- P48167 GLRB_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MKFLLTTAFLILISLWVEEAYSKEKSSKKGKGKKKQYLCPSQQSAEDLARVPANSTSNILNRLLVSYDPRIRPNFKGIPVDVVVNIFINSFGSIQETTMDYRVNIFLRQKWNDPRLKLPSDFRGSDALTVDPTMYKCLWKPDLFFANEKSANFHDVTQENILLFIFRDGDVLVSMRLSITLSCPLDLTLFPMDTQRCKMQLESFGYTTDDLRFIWQSGDPVQLEKIALPQFDIKKEDIEYGNCTKYYKGTGYYTCVEVIFTLRRQVGFYMMGVYAPTLLIVVLSWLSFWINPDASAARVPLGIFSVLSLASECTTLAAELPKVSYVKALDVWLIACLLFGFASLVEYAVVQVMLNNPKRVEAEKARIAKAEQADGKGGNVAKKNTVNGTGTPVHISTLQVGETRCKKVCTSKSDLRSNDFSIVGSLPRDFELSNYDCYGKPIEVNNGLGKSQAKNNKKPPPAKPVIPTAAKRIDLYARALFPFCFLFFNVIYWSIYL
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P48167 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K28 | Acetylation | Uniprot | |
| K31 | Acetylation | Uniprot | |
| K34 | Acetylation | Uniprot | |
| K35 | Acetylation | Uniprot | |
| K117 | Ubiquitination | Uniprot | |
| S125 | Phosphorylation | Uniprot | |
| T129 | Phosphorylation | Uniprot | |
| K136 | Ubiquitination | Uniprot | |
| K140 | Ubiquitination | Uniprot | |
| K198 | Ubiquitination | Uniprot | |
| S203 | Phosphorylation | Uniprot | |
| Y206 | Phosphorylation | Uniprot | |
| Y274 | Phosphorylation | Uniprot | |
| T277 | Phosphorylation | Uniprot | |
| K369 | Ubiquitination | Uniprot | |
| S411 | Phosphorylation | Uniprot | |
| Y435 | Phosphorylation | P12931 (SRC) | Uniprot |
| K440 | Ubiquitination | Uniprot |
研究背景
Glycine receptors are ligand-gated chloride channels. GLRB does not form ligand-gated ion channels by itself, but is part of heteromeric ligand-gated chloride channels. Channel opening is triggered by extracellular glycine. Heteropentameric channels composed of GLRB and GLRA1 are activated by lower glycine levels than homopentameric GLRA1. Plays an important role in the down-regulation of neuronal excitability. Contributes to the generation of inhibitory postsynaptic currents.
Cell junction>Synapse>Postsynaptic cell membrane>Multi-pass membrane protein. Cell junction>Synapse. Cell projection>Dendrite. Cell membrane>Multi-pass membrane protein. Cytoplasm.
Note: Retained in the cytoplasm upon heterologous expression by itself. Coexpression with GPHN promotes expression at the cell membrane (PubMed:12684523). Coexpression with GLRA1, GLRA2 or GLRA3 promotes expression at the cell membrane.
Heteropentamer composed of GLRB and GLRA1. Heteropentamer composed of GLRB and GLRA2. Heteropentamer composed of GLRB and GLRA3. Heteropentamer composed of two GLRA1 and three GLRB subunits. Heteropentamer composed of three GLRA1 and two GLRB subunits. Interacts with GPHN.
Belongs to the ligand-gated ion channel (TC 1.A.9) family. Glycine receptor (TC 1.A.9.3) subfamily. GLRB sub-subfamily.
研究领域
· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.
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