GABRA4 Antibody - #DF4069

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产品: GABRA4 抗体
货号: DF4069
描述: Rabbit polyclonal antibody to GABRA4
应用: WB IF/ICC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit
蛋白号: P48169
RRID: AB_2836443

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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实验方案
WB Handbook
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IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
GABRA4 Antibody detects endogenous levels of total GABRA4.
RRID:
AB_2836443
引用格式: Affinity Biosciences Cat# DF4069, RRID:AB_2836443.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

GABA(A) receptor subunit alpha 4; GABA(A) receptor subunit alpha-4; GABR A4; GABRA 4; Gabra4; Gamma aminobutyric acid (GABA) A receptor alpha 4; Gamma aminobutyric acid A receptor alpha 4; Gamma aminobutyric acid receptor alpha 4 subunit; Gamma aminobutyric acid receptor subunit alpha 4; Gamma-aminobutyric acid receptor subunit alpha-4; GBRA4_HUMAN;

抗原和靶标

免疫原:

A synthesized peptide derived from human GABRA4, corresponding to a region within N-terminal amino acids.

基因/基因ID:
GABRA4(2557)

研究领域

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Human Diseases > Substance dependence > Morphine addiction.

· Organismal Systems > Nervous system > Retrograde endocannabinoid signaling.   (View pathway)

· Organismal Systems > Nervous system > GABAergic synapse.

· Organismal Systems > Sensory system > Taste transduction.

文献引用

1). Enhanced axon guidance and synaptic markers in rat brains using ferric-tannic nanoparticles. Metallomics : integrated biometal science, 2024 (PubMed: 38936837) [IF=2.9]

Application: WB    Species: Rat    Sample:

Fig. 1(A) Western blotting analysis of Netrin-1 and GAPDH and relative changes in Netrin-1 expression with respect to GAPDH expression, reported as the Netrin-1/GAPDH ratio, in the cerebral cortexes of rats injected with ferric–tannic nanoparticles (FTs) at different times (n = 2). P = 0.0667, by one-way analysis of variance (ANOVA) with Kruskal–Wallis test. (B) Netrin-1 immunofluorescence staining of the cerebral cortex of control rats and rats injected with FTs at different times. (C) Individual quantitative analysis of Netrin-1 staining of four different areas of the cerebral cortex of control rats (n = 2) and five different areas of the cerebral cortex of rats injected with FTs for 48 hours (n = 2). (D) Semiquantitative analysis of Netrin-1 staining of the cerebral cortex of control rats (n = 2, total number of measured cells was 378), and rats injected with FTs for 48 hours (n = 2, total number of measured cells was 512). ****P < 0.0001 by unpaired t-test. (E) Western blotting analysis of GABRA4 and GAPDH and relative changes in Netrin-1 expression with respect to GAPDH expression, reported as the GABRA4/GAPDH ratio, in the cerebral cortex of rats injected with FTs at different times (n = 2). P = 0.933, by one-way ANOVA with Kruskal–Wallis test. (F) GABRA4 immunofluorescence staining of the cerebral cortex of control rats and rats injected with FTs at different times. (G) Individual quantitative analysis of GABRA4 staining of seven different areas of the cerebral cortex of control rats (n = 2) and five different areas of the cerebral cortex of rats injected with FTs for 48 hours (n = 2). (H) Semiquantitative analysis of Netrin-1 staining of the cerebral cortex of control rats (n = 2, total number of measured cells was 636), and rats injected with FTs for 48 hours (n = 2, total number of measured cells was 502). ****P < 0.0001 by unpaired t-test.

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