ERD22 Antibody - #DF4047

Fig. 2 KDELR2 functions as an oncogene in BCa. A-B Verification of KDELR2 expression in clinical tissues by qRT-PCR (normal, n = 24; tumor, n = 24) and immunohistochemical (IHC) staining (200x, 400x). C Detection of KDELR2 expression by western blot analysis in cell lines (n = 3). D, G Identification of knockdown or overexpression efficiency of KDELR2 by qRT-PCR and western blot analysis (n = 3). E, H Proliferation analysis of UMUC3 or T24 cells in the control (con) and siKDELR2-3 or KDELR2-overexpressing (ov) group (n = 4). (F, I) Migration and invasion (200x) of BCa cells in the control and siKDELR2-3 or KDELR2-overexpressing group (n = 3).

FIGURE 1 MUC5AC and KDELR2 expression levels are increased in patients with COPD. (A) Genes upregulated in healthy individuals (n = 40) and COPD patients (n = 111) from the GSE76925 dataset. (B) Relative KDELR1, KDELR2, and KDELR3 mRNA levels in healthy controls and patients with COPD in the GSE76925 dataset. (C) Haematoxylin and eosin images, AB-PAS and immunohistochemistry (IHC) analysis of MUC5AC in lung sections from the control group (n = 19) and COPD group (n = 18). The image parameters were as follows: 1600 × 1200, 72 pixels per inch (PPI). (D) The percentage of goblet cells in the lung field was determined. (E) Quantitative immunohistochemistry (IHC) analysis of MUC5AC in COPD patients (n = 18) and control subjects (n = 19). (F) Coimmunofluorescence staining of MUC5AC and KDELR2 in the lung tissues of controls and patients with COPD (2048 × 2048, 96 PPI). Scale bars: 50 μm. IOD: Integrated optical density. Data are shown as the mean ± SEM. *p 

FIGURE 5 The IRE1α/XBP-1 s pathway influences KDELR2 expression during MUC5AC overproduction in the airway. (A) Schematic diagram of pharmacological inhibition in rats. (B) The expression of IRE1α, p-IRE1α, XBP-1 s and KDELR2 in the lungs of the control group, COPD group, COPD group treated with DMSO and p-IRE1α inhibitor 4μ8C determined by Western blotting (n = 6). (C–E) Relative protein expression of p-IRE1α/IRE1α, XBP-1 s and KDELR2 measured by Western blotting (n = 6). (F) The relative expression of MUC5AC in BALF was determined by ELISA (n = 6). (G) Relative mRNA expression of MUC5AC in lung tissues measured by RT–qPCR (n = 6). Data are shown as the mean ± SEM; *p