NEIL1 Antibody - #DF4041

Fig. 1: CRISPR screening identifies epigenetic candidates involved in regulation of IFN-β-mediated antiviral response. a Schematic diagram of the genome-wide CRISPR/Cas9 screening. Stable cell lines were obtained by transfection of A549 cells with pcDNA4/TO-IFN-β-promoter-mCherry plasmids and screening with zeocin. Then, a library of CRISPR/Cas9 KO-screened A549 overexpressing cells was infected with PR8 (1 MOI). Cells with different fluorescence signals were subsequently obtained by flow cytometric sorting, and gDNA was extracted for sequence analysis. Genes enriched were then compared with those from uninfected cells. b Volcano plots identifying candidate genes enriched upon positive and negative selection identified. c The bubble diagram of enrichment analysis on gene functions. Candidate genes positive screening: filter candidate genes by pos|rank, pos|p value