DNAJC5 Antibody - #DF4011

Fig. 2 Overexpression of DNAJC5 promotes DDP resistance in EOC cells in vitro. (a) Expression levels of DNAJC5 in different EOC cell lines. (b) Western blot analysis confirming DNAJC5 protein expression in A2780 cells infected with a lentivirus overexpressing DNAJC5. (c) A2780 and A2780/DNAJC5 cells were incubated with varying concentrations of DDP (50µM, 40µM, 30µM, 20µM, 10µM, 5 µM, 2.5µM, 1.25µM) for 72 h. Cells were then treated with CCK-8 reagent in the dark for 1.5 h, and the final IC50 values were determined. (d,e) Clonogenic assay of A2780/DNAJC5 cells. A total of 300 cells per well were seeded and treated with 3µM DDP after 24 h. After 10 days, colonies were stained with crystal violet and quantified using ImageJ software. The bar graph represents the effect of DDP on the viability of A2780 and A2780/DNAJC5 cells. (f,g) Apoptosis assay of A2780/DNAJC5 cells following treatment with 3µM DDP for 72 h. The bar graph illustrates the average apoptosis rate of A2780/DNAJC5 cells under DDP treatment compared to the Vector control.