产品: GNPAT 抗体
货号: DF3993
描述: Rabbit polyclonal antibody to GNPAT
应用: WB IF/ICC
反应: Human
预测: Rat, Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 77 KD; 77kD(Calculated).
蛋白号: O15228
RRID: AB_2836353

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human
预测:
Rat(%), Pig(100%), Bovine(91%), Horse(100%), Sheep(91%), Rabbit(100%), Dog(100%)
克隆:
Polyclonal
特异性:
GNPAT Antibody detects endogenous levels of total GNPAT.
RRID:
AB_2836353
引用格式: Affinity Biosciences Cat# DF3993, RRID:AB_2836353.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Acyl CoA dihydroxyacetonephosphateacyltransferase; Acyl-CoA:dihydroxyacetonephosphateacyltransferase; DAP AT; DAP-AT; DAPAT; DHAP AT; DHAP-AT; DHAPAT; Dihydroxyacetone phosphate acyltransferase; EC 2.3.1.42; Glycerone-phosphate O-acyltransferase; Glyceronephosphate O acyltransferase; GNPAT; GNPAT_HUMAN; OTTHUMP00000036147;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
序列:
MESSSSSNSYFSVGPTSPSAVVLLYSKELKKWDEFEDILEERRHVSDLKFAMKCYTPLVYKGITPCKPIDIKCSVLNSEEIHYVIKQLSKESLQSVDVLREEVSEILDEMSHKLRLGAIRFCAFTLSKVFKQIFSKVCVNEEGIQKLQRAIQEHPVVLLPSHRSYIDFLMLSFLLYNYDLPVPVIAAGMDFLGMKMVGELLRMSGAFFMRRTFGGNKLYWAVFSEYVKTMLRNGYAPVEFFLEGTRSRSAKTLTPKFGLLNIVMEPFFKREVFDTYLVPISISYDKILEETLYVYELLGVPKPKESTTGLLKARKILSENFGSIHVYFGDPVSLRSLAAGRMSRSSYNLVPRYIPQKQSEDMHAFVTEVAYKMELLQIENMVLSPWTLIVAVLLQNRPSMDFDALVEKTLWLKGLTQAFGGFLIWPDNKPAEEVVPASILLHSNIASLVKDQVILKVDSGDSEVVDGLMLQHITLLMCSAYRNQLLNIFVRPSLVAVALQMTPGFRKEDVYSCFRFLRDVFADEFIFLPGNTLKDFEEGCYLLCKSEAIQVTTKDILVTEKGNTVLEFLVGLFKPFVESYQIICKYLLSEEEDHFSEEQYLAAVRKFTSQLLDQGTSQCYDVLSSDVQKNALAACVRLGVVEKKKINNNCIFNVNEPATTKLEEMLGCKTPIGKPATAKL

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Dog
100
Rabbit
100
Bovine
91
Sheep
91
Zebrafish
64
Xenopus
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - O15228 作为底物

Site PTM Type Enzyme
S17 Phosphorylation
K31 Ubiquitination
K49 Ubiquitination
K67 Ubiquitination
K90 Ubiquitination
S111 Phosphorylation
K113 Ubiquitination
K128 Acetylation
K136 Ubiquitination
K146 Ubiquitination
K251 Ubiquitination
T291 Phosphorylation
K304 Ubiquitination
K312 Ubiquitination
S345 Phosphorylation
S346 Phosphorylation
S589 Phosphorylation
K606 Ubiquitination
S625 Phosphorylation
K643 Acetylation
K644 Acetylation
K674 Ubiquitination

研究背景

细胞定位:

Peroxisome membrane>Peripheral membrane protein>Matrix side.
Note: Exclusively localized to the lumenal side of the peroxisomal membrane.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

May be part of a heterotrimeric complex composed of DAP-AT, ADAP-S and a modified form of DAP-AT.

蛋白家族:

The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.

Belongs to the GPAT/DAPAT family.

研究领域

· Cellular Processes > Transport and catabolism > Peroxisome.   (View pathway)

· Metabolism > Lipid metabolism > Glycerophospholipid metabolism.

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