Phospho-Retinoblastoma (Thr826) Antibody - #AF0030

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产品: 磷酸化 Retinoblastoma (Thr826) 抗体
货号: AF0030
描述: Rabbit polyclonal antibody to Phospho-Retinoblastoma (Thr826)
应用: WB IF/ICC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
蛋白号: P06400
RRID: AB_2833290

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 50ul RMB¥ 1300 现货
 100ul RMB¥ 2400 现货
 200ul RMB¥ 3200 现货

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说明书
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Phospho-Retinoblastoma (Thr826) Antibody detects endogenous levels of Retinoblastoma only when phosphorylated at Threonine 826.
RRID:
AB_2833290
引用格式: Affinity Biosciences Cat# AF0030, RRID:AB_2833290.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Exon 17 tumor GOS561 substitution mutation causes premature stop; GOS563 exon 17 substitution mutation causes premature stop; OSRC; Osteosarcoma; p105-Rb; P105RB; PP105; pp110; PPP1R130; pRb; Prepro retinoblastoma associated protein; Protein phosphatase 1 regulatory subunit 130; Rb; RB transcriptional corepressor 1; RB_HUMAN; RB1; RB1 gene; Retinoblastoma 1; Retinoblastoma suspectibility protein; Retinoblastoma-associated protein;

抗原和靶标

免疫原:

A synthesized peptide derived from human Retinoblastoma around the phosphorylation site of Thr826.

基因/基因ID:
RB1(5925)
描述:
Retinoblastoma (RB) is an embryonic malignant neoplasm of retinal origin. It almost always presents in early childhood and is often bilateral. Spontaneous regression ('cure') occurs in some cases.

研究领域

· Cellular Processes > Cell growth and death > Cell cycle.   (View pathway)

· Cellular Processes > Cell growth and death > Cellular senescence.   (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > Endocrine resistance.

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

· Human Diseases > Cancers: Specific types > Pancreatic cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Glioma.   (View pathway)

· Human Diseases > Cancers: Specific types > Prostate cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Melanoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Bladder cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Chronic myeloid leukemia.   (View pathway)

· Human Diseases > Cancers: Specific types > Small cell lung cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Non-small cell lung cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Breast cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Gastric cancer.   (View pathway)

文献引用

1). IGFBP-rP1 acts as a potential tumor suppressor via the suppression of ERK signaling pathway in endometrial cancer cells. Molecular Medicine Reports, 2017 (PubMed: 29067463) [IF=3.4]

Application: WB    Species: human    Sample:

Figure 3. Differential expression patterns of p-RB, p16, p53, p21 and p-ERK in endometrial cancer cells. (A) Western blotting images of related proteins.
2). LncRNA MALAT1 was regulated by HPV16 E7 independently of pRB in cervical cancer cells. Journal of Cancer, 2021 (PubMed: 34659524) [IF=3.3]

Application: WB    Species: Human    Sample: CC and HPV positive cervical epithelia tissues

Figure 1 MALAT1 was upregulated by HPV16 E7. A. The level of MALAT1 RNA transcript were elevated in human CC and HPV positive cervical epithelia tissues.MALAT1 RNA levels in human cervical tissues were detected by RT-qPCR. B. By RT-qPCR, lncRNA MALAT1 was shown up-expressed in high-risk HPV infected cervical cancer cells HeLa (HPV18 positive), SiHa and Caski (HPV16 positive), compared with C33A (HPV negative) cells and 293T. C. Overexpression of HPV16 E7 upregulated MALAT1 when pCMV-E7 transfected 293T and C33A cells and RT-qPCR was performed. D. Depletion of HPV16 E7 with siRNA against E7 transfection significantly decreased MALAT1 level. E. With HPV16 E7 intrabody VHH2 expressing plasmid transfection in SiHa and Caski, pRB was increased, and MALAT1 RNA was decreased. Left: western blot. Right: RT-qPCR analysis. Similar results were obtained in at least three independent experiments. GAPDH mRNA was used to normalize the qRT-PCR analyses. *** p ≤ 0.001.
3). Lappaconitine sulfate inhibits proliferation and induces mitochondrial-mediated apoptosis via regulating PI3K/AKT/GSK3β signaling pathway in HeLa cells. Naunyn-Schmiedeberg's Archives of Pharmacology, 2023 (PubMed: 37306713) [IF=3.1]

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