产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# BF0551, RRID:AB_2833566.
展开/折叠
Amine oxidase (flavin containing) domain 2; Amine oxidase, flavin containing, 2; AOF2; BHC110; BRAF35 HDAC complex protein BHC110; BRAF35-HDAC complex protein BHC110; BRAF35/HDAC complex, 110-kD subunit; CPRF; EC1; FAD binding protein BRAF35 HDAC complex, 110 kDa subunit; Flavin-containing amine oxidase domain-containing protein 2; KDM 1; KDM1; Kdm1a; KDM1A_HUMAN; KIAA0601; LSD 1; LSD1; Lysine (K) specific demethylase 1; Lysine (K) specific demethylase 1A; Lysine demethylase 1A; Lysine specific histone demethylase 1; Lysine specific histone demethylase 1A; Lysine-specific demethylase 1; Lysine-specific demethylase 1A; Lysine-specific histone demethylase 1A;
抗原和靶标
Purified recombinant fragment of human LSD1/AOF2 expressed in E. Coli.
- O60341 KDM1A_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MLSGKKAAAAAAAAAAAATGTEAGPGTAGGSENGSEVAAQPAGLSGPAEVGPGAVGERTPRKKEPPRASPPGGLAEPPGSAGPQAGPTVVPGSATPMETGIAETPEGRRTSRRKRAKVEYREMDESLANLSEDEYYSEEERNAKAEKEKKLPPPPPQAPPEEENESEPEEPSGVEGAAFQSRLPHDRMTSQEAACFPDIISGPQQTQKVFLFIRNRTLQLWLDNPKIQLTFEATLQQLEAPYNSDTVLVHRVHSYLERHGLINFGIYKRIKPLPTKKTGKVIIIGSGVSGLAAARQLQSFGMDVTLLEARDRVGGRVATFRKGNYVADLGAMVVTGLGGNPMAVVSKQVNMELAKIKQKCPLYEANGQAVPKEKDEMVEQEFNRLLEATSYLSHQLDFNVLNNKPVSLGQALEVVIQLQEKHVKDEQIEHWKKIVKTQEELKELLNKMVNLKEKIKELHQQYKEASEVKPPRDITAEFLVKSKHRDLTALCKEYDELAETQGKLEEKLQELEANPPSDVYLSSRDRQILDWHFANLEFANATPLSTLSLKHWDQDDDFEFTGSHLTVRNGYSCVPVALAEGLDIKLNTAVRQVRYTASGCEVIAVNTRSTSQTFIYKCDAVLCTLPLGVLKQQPPAVQFVPPLPEWKTSAVQRMGFGNLNKVVLCFDRVFWDPSVNLFGHVGSTTASRGELFLFWNLYKAPILLALVAGEAAGIMENISDDVIVGRCLAILKGIFGSSAVPQPKETVVSRWRADPWARGSYSYVAAGSSGNDYDLMAQPITPGPSIPGAPQPIPRLFFAGEHTIRNYPATVHGALLSGLREAGRIADQFLGAMYTLPRQATPGVPAQQSPSM
翻译修饰 - O60341 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T59 | Phosphorylation | Uniprot | |
| S69 | Phosphorylation | Uniprot | |
| S80 | Phosphorylation | Uniprot | |
| T104 | Phosphorylation | Uniprot | |
| T110 | Phosphorylation | Uniprot | |
| S111 | Phosphorylation | Uniprot | |
| K114 | Methylation | Uniprot | |
| K117 | Sumoylation | Uniprot | |
| S126 | Phosphorylation | Uniprot | |
| S131 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| Y135 | Phosphorylation | Uniprot | |
| Y136 | Phosphorylation | Uniprot | |
| S137 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| S166 | Phosphorylation | Uniprot | |
| S172 | Phosphorylation | Uniprot | |
| R187 | Methylation | Uniprot | |
| K268 | Ubiquitination | Uniprot | |
| K271 | Ubiquitination | Uniprot | |
| T275 | Phosphorylation | Uniprot | |
| K280 | Ubiquitination | Uniprot | |
| S299 | Phosphorylation | Uniprot | |
| T305 | Phosphorylation | Uniprot | |
| K322 | Methylation | Uniprot | |
| K322 | Ubiquitination | Uniprot | |
| T335 | Phosphorylation | Uniprot | |
| S346 | Phosphorylation | Uniprot | |
| K347 | Ubiquitination | Uniprot | |
| K355 | Ubiquitination | Uniprot | |
| K359 | Ubiquitination | Uniprot | |
| Y363 | Phosphorylation | Uniprot | |
| K372 | Ubiquitination | Uniprot | |
| K374 | Ubiquitination | Uniprot | |
| K424 | Sumoylation | Uniprot | |
| K424 | Ubiquitination | Uniprot | |
| K432 | Acetylation | Uniprot | |
| K432 | Ubiquitination | Uniprot | |
| K433 | Acetylation | Uniprot | |
| K436 | Acetylation | Uniprot | |
| K436 | Ubiquitination | Uniprot | |
| K442 | Sumoylation | Uniprot | |
| K442 | Ubiquitination | Uniprot | |
| K447 | Ubiquitination | Uniprot | |
| K452 | Sumoylation | Uniprot | |
| K452 | Ubiquitination | Uniprot | |
| K456 | Ubiquitination | Uniprot | |
| K463 | Ubiquitination | Uniprot | |
| S466 | Phosphorylation | Uniprot | |
| K469 | Ubiquitination | Uniprot | |
| K481 | Ubiquitination | Uniprot | |
| K492 | Ubiquitination | Uniprot | |
| K503 | Ubiquitination | Uniprot | |
| K507 | Ubiquitination | Uniprot | |
| K585 | Sumoylation | Uniprot | |
| K585 | Ubiquitination | Uniprot | |
| T588 | Phosphorylation | Uniprot | |
| S611 | Phosphorylation | Uniprot | |
| K617 | Ubiquitination | Uniprot | |
| K631 | Ubiquitination | Uniprot | |
| K647 | Ubiquitination | Uniprot | |
| K661 | Ubiquitination | Uniprot | |
| S683 | Phosphorylation | P49841 (GSK3B) | Uniprot |
| S687 | Phosphorylation | Uniprot | |
| K732 | Ubiquitination | Uniprot | |
| K744 | Ubiquitination | Uniprot | |
| R838 | Methylation | Uniprot | |
| T841 | Phosphorylation | Uniprot | |
| S849 | Phosphorylation | Uniprot | |
| S851 | Phosphorylation | Uniprot |
研究背景
Histone demethylase that can demethylate both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. Also acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in ANDR-containing complexes, which mediates phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A. Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation. Demethylates and stabilizes the DNA methylase DNMT1. Required for gastrulation during embryogenesis. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. Effector of SNAI1-mediated transcription repression of E-cadherin/CDH1, CDN7 and KRT8. Required for the maintenance of the silenced state of the SNAI1 target genes E-cadherin/CDH1 and CDN7.
Polyubiquitinated by JADE2; which leads to its proteasomal degradation.
Nucleus.
Ubiquitously expressed.
Component of a histone demethylase complex with RCOR1. Component of a RCOR/GFI/KDM1A/HDAC complex. Interacts directly with GFI1 and GFI1B. Interacts with INSM1 (via N-terminus). Component of a BHC histone deacetylase complex that contains HDAC1, HDAC2, HMG20B, KDM1A, RCOR1 and PHF21A. The BHC complex may also contain ZMYM2, ZNF217, ZMYM3, GSE1 and GTF2I. In the complex, RCOR1/CoREST strongly enhances the demethylase activity and protects it from the proteasome while PHF21A/BHC80 inhibits the demethylase activity. Interacts with the androgen receptor (AR). Interacts with SNAI1 (via SNAG domain). Interacts (via AOD/Tower domain) with JADE2 (via C-terminus). Interacts with ESRRB; co-occupes the core set of ESRRB targets (By similarity).
The SWIRM domain may act as an anchor site for a histone tail.
Belongs to the flavin monoamine oxidase family.
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