CHST13 Antibody - #DF3882

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产品: CHST13 抗体
货号: DF3882
描述: Rabbit polyclonal antibody to CHST13
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Chicken
分子量: 39 KD; 39kD(Calculated).
蛋白号: Q8NET6
RRID: AB_2836235

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(80%), Bovine(90%), Chicken(90%)
克隆:
Polyclonal
特异性:
CHST13 Antibody detects endogenous levels of total CHST13.
RRID:
AB_2836235
引用格式: Affinity Biosciences Cat# DF3882, RRID:AB_2836235.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

C4ST 3; C4ST-3; C4ST3; Carbohydrate (chondroitin 4) sulfotransferase 13; Carbohydrate sulfotransferase 13; Chondroitin 4 O sulfotransferase 3; Chondroitin 4 sulfotransferase 3; Chondroitin 4-O-sulfotransferase 3; Chondroitin 4-sulfotransferase 3; CHST 13; CHST13; CHSTD_HUMAN; MGC119278; MGC119279; MGC119281;

抗原和靶标

免疫原:
Uniprot:

Q8NET6(CHSTD_HUMAN) >>Visit HPA database.

基因/基因ID:
CHST13(166012)
表达:
Q8NET6 CHSTD_HUMAN:

Highly expressed in adult liver. Expressed at lower level in kidney, lymph nodes and fetal kidney.

序列:
MGRRCCRRRVLAAACLGAALLLLCAAPRSLRPAFGNRALGSSWLGGEKRSPLQKLYDLDQDPRSTLAKVHRQRRDLLNSACSRHSRRQRLLQPEDLRHVLVDDAHGLLYCYVPKVACTNWKRVLLALSGQARGDPRAISAQEAHAPGRLPSLADFSPAEINRRLRAYLAFLFVREPFERLASAYRNKLARPYSAAFQRRYGARIVQRLRPRALPDARARGHDVRFAEFLAYLLDPRTRREEPFNEHWERAHALCHPCRLRYDVVGKFETLAEDAAFVLGLAGASDLSFPGPPRPRGAAASRDLAARLFRDISPFYQRRLFDLYKMDFLLFNYSAPSYLRLL

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Bovine
90
Chicken
90
Pig
80
Xenopus
70
Dog
57
Horse
0
Sheep
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q8NET6 作为底物

Site PTM Type Enzyme
S139 Phosphorylation
Y200 Phosphorylation
Y337 Phosphorylation

研究背景

功能:

Catalyzes the transfer of sulfate to position 4 of the N-acetylgalactosamine (GalNAc) residue of chondroitin. Chondroitin sulfate constitutes the predominant proteoglycan present in cartilage and is distributed on the surfaces of many cells and extracellular matrices. Transfers sulfate to the C4 hydroxyl of beta1,4-linked GalNAc that is substituted with a beta-linked glucuronic acid at the C-3 hydroxyl. No activity toward dermatan.

细胞定位:

Golgi apparatus membrane>Single-pass type II membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Highly expressed in adult liver. Expressed at lower level in kidney, lymph nodes and fetal kidney.

蛋白家族:

Belongs to the sulfotransferase 2 family.

研究领域

· Metabolism > Glycan biosynthesis and metabolism > Glycosaminoglycan biosynthesis - chondroitin sulfate / dermatan sulfate.

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