产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF3839, RRID:AB_2836196.
展开/折叠
384.Beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase; 4-galactosyltransferase 1; 4-GalTase 1; b4Gal-T1; B4galt1; B4GT1_HUMAN; Beta-1; Beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase; Beta4Gal-T1; CDG2D; GGTB2; GT1; GTB; Lactose synthase A protein; N-acetyllactosamine synthase; Nal synthase; Processed beta-1; UDP-Gal:beta-GlcNAc beta-1; UDP-galactose:beta-N-acetylglucosamine beta-1;
抗原和靶标
- P15291 B4GT1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MRLREPLLSGSAAMPGASLQRACRLLVAVCALHLGVTLVYYLAGRDLSRLPQLVGVSTPLQGGSNSAAAIGQSSGELRTGGARPPPPLGASSQPRPGGDSSPVVDSGPGPASNLTSVPVPHTTALSLPACPEESPLLVGPMLIEFNMPVDLELVAKQNPNVKMGGRYAPRDCVSPHKVAIIIPFRNRQEHLKYWLYYLHPVLQRQQLDYGIYVINQAGDTIFNRAKLLNVGFQEALKDYDYTCFVFSDVDLIPMNDHNAYRCFSQPRHISVAMDKFGFSLPYVQYFGGVSALSKQQFLTINGFPNNYWGWGGEDDDIFNRLVFRGMSISRPNAVVGRCRMIRHSRDKKNEPNPQRFDRIAHTKETMLSDGLNSLTYQVLDVQRYPLYTQITVDIGTPS
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P15291 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S73 | Phosphorylation | Uniprot | |
| S74 | Phosphorylation | Uniprot | |
| R204 | Methylation | Uniprot | |
| S270 | Phosphorylation | Uniprot | |
| S344 | Phosphorylation | Uniprot | |
| S373 | Phosphorylation | Uniprot |
研究背景
The Golgi complex form catalyzes the production of lactose in the lactating mammary gland and could also be responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.
The cell surface form functions as a recognition molecule during a variety of cell to cell and cell to matrix interactions, as those occurring during development and egg fertilization, by binding to specific oligosaccharide ligands on opposing cells or in the extracellular matrix.
The soluble form derives from the membrane forms by proteolytic processing.
Golgi apparatus>Golgi stack membrane>Single-pass type II membrane protein. Cell membrane>Single-pass type II membrane protein. Cell surface. Cell projection>Filopodium.
Note: Found in trans cisternae of Golgi but is mainly localized at the plasma membrane (PubMed:1714903). B4GALT1 cell surface expression is regulated by UBE2Q1 (By similarity).
Golgi apparatus>Golgi stack membrane>Single-pass type II membrane protein.
Note: Found in trans cisternae of Golgi.
Secreted.
Note: Soluble form found in body fluids.
Ubiquitously expressed, but at very low levels in fetal and adult brain.
Homodimer; and heterodimer with alpha-lactalbumin to form lactose synthase. Interacts (via N-terminal cytoplasmic domain) with UBE2Q1 (via N-terminus); the interaction is direct (By similarity).
Belongs to the glycosyltransferase 7 family.
研究领域
· Metabolism > Carbohydrate metabolism > Galactose metabolism.
· Metabolism > Glycan biosynthesis and metabolism > N-Glycan biosynthesis.
· Metabolism > Glycan biosynthesis and metabolism > Other types of O-glycan biosynthesis.
· Metabolism > Glycan biosynthesis and metabolism > Glycosaminoglycan biosynthesis - keratan sulfate.
· Metabolism > Glycan biosynthesis and metabolism > Glycosphingolipid biosynthesis - lacto and neolacto series.
· Metabolism > Global and overview maps > Metabolic pathways.
限制条款
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