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| 产品: | ATG16L1 抗体 |
| 货号: | DF3825 |
| 描述: | Rabbit polyclonal antibody to ATG16L1 |
| 应用: | WB IF/ICC |
| 反应: | Human, Mouse |
| 预测: | Pig, Bovine, Horse, Sheep, Rabbit, Dog |
| 分子量: | 69 KD; 68kD(Calculated). |
| 蛋白号: | Q676U5 |
| RRID: | AB_2836182 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF3825, RRID:AB_2836182.
展开/折叠
A16L1_HUMAN; APG16 like 1; APG16-like 1; APG16L; APG16L beta; ATG16 autophagy related 16 like 1; ATG16 autophagy related 16-like 1 (S. cerevisiae); ATG16A; ATG16L; Atg16l1; Autophagy related protein 16 1; Autophagy-related protein 16-1; FLJ00045; FLJ10035; FLJ10828; FLJ22677; IBD10; OTTHUMP00000164391; OTTHUMP00000164393; OTTHUMP00000165876; OTTHUMP00000165877; WD repeat domain 30; WDR30;
抗原和靶标
- Q676U5 A16L1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSSGLRAADFPRWKRHISEQLRRRDRLQRQAFEEIILQYNKLLEKSDLHSVLAQKLQAEKHDVPNRHEISPGHDGTWNDNQLQEMAQLRIKHQEELTELHKKRGELAQLVIDLNNQMQRKDREMQMNEAKIAECLQTISDLETECLDLRTKLCDLERANQTLKDEYDALQITFTALEGKLRKTTEENQELVTRWMAEKAQEANRLNAENEKDSRRRQARLQKELAEAAKEPLPVEQDDDIEVIVDETSDHTEETSPVRAISRAATKRLSQPAGGLLDSITNIFGRRSVSSFPVPQDNVDTHPGSGKEVRVPATALCVFDAHDGEVNAVQFSPGSRLLATGGMDRRVKLWEVFGEKCEFKGSLSGSNAGITSIEFDSAGSYLLAASNDFASRIWTVDDYRLRHTLTGHSGKVLSAKFLLDNARIVSGSHDRTLKLWDLRSKVCIKTVFAGSSCNDIVCTEQCVMSGHFDKKIRFWDIRSESIVREMELLGKITALDLNPERTELLSCSRDDLLKVIDLRTNAIKQTFSAPGFKCGSDWTRVVFSPDGSYVAAGSAEGSLYIWSVLTGKVEKVLSKQHSSSINAVAWSPSGSHVVSVDKGCKAVLWAQY
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q676U5 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S18 | Phosphorylation | Uniprot | |
| K45 | Ubiquitination | Uniprot | |
| K55 | Ubiquitination | Uniprot | |
| K60 | Ubiquitination | Uniprot | |
| S70 | Phosphorylation | Uniprot | |
| T76 | Phosphorylation | Uniprot | |
| K91 | Ubiquitination | Uniprot | |
| S139 | Phosphorylation | Uniprot | |
| K151 | Methylation | Uniprot | |
| K198 | Ubiquitination | Uniprot | |
| K222 | Ubiquitination | Uniprot | |
| T247 | Phosphorylation | Uniprot | |
| T254 | Phosphorylation | Uniprot | |
| S255 | Phosphorylation | Uniprot | |
| T265 | Phosphorylation | Uniprot | |
| S269 | Phosphorylation | Uniprot | |
| S278 | Phosphorylation | Uniprot | |
| S287 | Phosphorylation | Uniprot | |
| S289 | Phosphorylation | Uniprot | |
| S290 | Phosphorylation | Uniprot | |
| T300 | Phosphorylation | Uniprot | |
| S304 | Phosphorylation | Uniprot | |
| S331 | Phosphorylation | Uniprot | |
| K513 | Ubiquitination | Uniprot | |
| K523 | Ubiquitination | Uniprot |
研究背景
Plays an essential role in autophagy: interacts with ATG12-ATG5 to mediate the conjugation of phosphatidylethanolamine (PE) to LC3 (MAP1LC3A, MAP1LC3B or MAP1LC3C), to produce a membrane-bound activated form of LC3 named LC3-II. Thereby, controls the elongation of the nascent autophagosomal membrane. Regulates mitochondrial antiviral signaling (MAVS)-dependent type I interferon (IFN-I) production. Negatively regulates NOD1- and NOD2-driven inflammatory cytokine response. Instead, promotes with NOD2 an autophagy-dependent antibacterial pathway. Plays a role in regulating morphology and function of Paneth cell.
Proteolytic cleavage by activated CASP3 leads to degradation and may regulate autophagy upon cellular stress and apoptotic stimuli.
Phosphorylation at Ser-139 promotes association with the ATG12-ATG5 conjugate to form the ATG12-ATG5-ATG16L1 complex.
Cytoplasm. Preautophagosomal structure membrane>Peripheral membrane protein.
Note: Recruited to omegasomes membranes by WIPI2. Omegasomes are endoplasmic reticulum connected strutures at the origin of preautophagosomal structures. Localized to preautophagosomal structure (PAS) where it is involved in the membrane targeting of ATG5. Localizes also to discrete punctae along the ciliary axoneme.
Homodimer. Homooligomer (By similarity). Interacts with WIPI1. Interacts with WIPI2. Interacts with RB1CC1; the interaction is required for ULK1 complex-dependent autophagy. Interacts with ATG5. Part of either the minor and major complexes respectively composed of 4 sets of ATG12-ATG5 and ATG16L1 (400 kDa) or 8 sets of ATG12-ATG5 and ATG16L1 (800 kDa). Interacts with RAB33B. Interacts (via WD repeats) with TMEM59; the interaction mediates unconventional autophagic activity of TMEM59. Interacts with TLR2 and NOD2. Interacts (via WD repeats) with MEFV. Interacts with PPP1CA; the interaction dephosphorylates ATG16L1 causing dissociation of ATG12-ATG5-ATG16L1 complex. Interacts (via N-terminal) with CLTC. Interacts with NOD2. Interacts with TUFM. Interacts with TRIM16.
Belongs to the WD repeat ATG16 family.
研究领域
· Cellular Processes > Transport and catabolism > Autophagy - other. (View pathway)
· Cellular Processes > Transport and catabolism > Autophagy - animal. (View pathway)
· Organismal Systems > Immune system > NOD-like receptor signaling pathway. (View pathway)
文献引用
Application: WB Species: Mouse Sample: brain tissue
限制条款
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