LYPLA1 Antibody - #DF3737

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产品: LYPLA1 抗体
货号: DF3737
描述: Rabbit polyclonal antibody to LYPLA1
应用: WB IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 25 KD; 25kD(Calculated).
蛋白号: O75608
RRID: AB_2836101

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(91%), Zebrafish(100%), Bovine(100%), Horse(91%), Sheep(100%), Rabbit(100%), Dog(91%), Chicken(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
LYPLA1 Antibody detects endogenous levels of total LYPLA1.
RRID:
AB_2836101
引用格式: Affinity Biosciences Cat# DF3737, RRID:AB_2836101.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Acyl protein thioesterase 1; Acyl-protein thioesterase 1; APT 1; APT-1; APT1; hAPT1; LPL-I; LPL1; LYPA1_HUMAN; LYPLA 1; LYPLA1; Lysophospholipase 1; Lysophospholipase I; Lysophospholipid specific lysophospholipase; LYSOPLA; LysoPLA I;

抗原和靶标

免疫原:
Uniprot:

O75608(LYPA1_HUMAN) >>Visit HPA database.

基因/基因ID:
LYPLA1(10434)
序列:
MCGNNMSTPLPAIVPAARKATAAVIFLHGLGDTGHGWAEAFAGIRSSHIKYICPHAPVRPVTLNMNVAMPSWFDIIGLSPDSQEDESGIKQAAENIKALIDQEVKNGIPSNRIILGGFSQGGALSLYTALTTQQKLAGVTALSCWLPLRASFPQGPIGGANRDISILQCHGDCDPLVPLMFGSLTVEKLKTLVNPANVTFKTYEGMMHSSCQQEMMDVKQFIDKLLPPID

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Bovine
100
Sheep
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Pig
91
Horse
91
Dog
91
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - O75608 作为底物

Site PTM Type Enzyme
K97 Ubiquitination
K105 Ubiquitination
K190 Ubiquitination
S209 Phosphorylation
S210 Phosphorylation
C211 S-Nitrosylation
K224 Ubiquitination

研究背景

功能:

Hydrolyzes fatty acids from S-acylated cysteine residues in proteins such as trimeric G alpha proteins or HRAS. Has depalmitoylating activity toward KCNMA1. Has low lysophospholipase activity.

细胞定位:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Homodimer.

蛋白家族:

Belongs to the AB hydrolase superfamily. AB hydrolase 2 family.

研究领域

· Human Diseases > Cancers: Overview > Choline metabolism in cancer.   (View pathway)

· Metabolism > Lipid metabolism > Glycerophospholipid metabolism.

文献引用

1). Renal lysophospholipase A1 contributes to Enterococcus faecalis-induced hypertension by enhancing sodium reabsorption. iScience (PubMed: 36419851) [IF=5.8]

Application: WB    Species: Mouse    Sample: renal medulla

Figure 1 E. faecalis treatment increased blood pressure and renal LYPLA1 expression in mice (A) Systolic blood pressure (SBP, n = 5 per group) and diastolic blood pressure (DBP, n = 5 per group). (B) Serum Ang II levels (n = 5 per group). (C) Representative immunoblots of LYPLA1 and summarized intensities of blots in the renal medulla (n = 5 per group). (D) mRNA levels of LYPLA1 in the renal medulla (n = 5 per group). (E) Urinary LYPLA1 content (n = 5 per group). (F) Representative immunochemistry staining of LYPLA1 in the kidney and summarized intensities of positive staining (n = 5 per group, bar = 100 μm). Data are presented as mean ± SE. ∗p < 0.05, ∗∗p < 0.01; ns, no significance.

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