PTPN22 Antibody - #AF0391

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产品: PTPN22 抗体
货号: AF0391
描述: Rabbit polyclonal antibody to PTPN22
应用: WB IF/ICC
反应: Human, Mouse, Rat
预测: Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 91kDa; 92kD(Calculated).
蛋白号: Q9Y2R2
RRID: AB_2833545

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Zebrafish(83%), Bovine(100%), Horse(100%), Sheep(92%), Rabbit(100%), Dog(92%), Chicken(92%)
克隆:
Polyclonal
特异性:
PTPN22 Antibody detects endogenous levels of total PTPN22.
RRID:
AB_2833545
引用格式: Affinity Biosciences Cat# AF0391, RRID:AB_2833545.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Hematopoietic cell protein tyrosine phosphatase 70Z PEP; Hematopoietic cell protein-tyrosine phosphatase 70Z-PEP; Lymphoid phosphatase; Lymphoid specific protein tyrosine phosphatase; lymphoid-specific, inducible human protein tyrosine phosphatase; Lyp 1; Lyp 2; LyP; Lyp1; Lyp2; LyPTP; OTTHUMP00000013720; OTTHUMP00000013721; OTTHUMP00000233450; OTTHUMP00000233451; PEP; PEST-domain phosphatase; Protein tyrosine phosphatase non receptor type 22 (lymphoid); Protein tyrosine phosphatase non receptor type 22; Protein tyrosine phosphatase non receptor type 8; Protein tyrosine phosphatase non receptor type 8, formerly; PTN22_HUMAN; PTPN 22; PTPN 8; Ptpn22; PTPN8; PTPN8, formerly; Tyrosine-protein phosphatase non-receptor type 22;

抗原和靶标

免疫原:
Uniprot:

Q9Y2R2(PTN22_HUMAN) >>Visit HPA database.

基因/基因ID:
PTPN22(26191)
表达:
Q9Y2R2 PTN22_HUMAN:

Expressed in bone marrow, B and T-cells, PBMCs, natural killer cells, monocytes, dendritic cells and neutrophils (PubMed:15208781). Both isoform 1 and 4 are predominantly expressed in lymphoid tissues and cells. Isoform 1 is expressed in thymocytes and both mature B and T-cells.

描述:
PTPN22 Seems to act on Cbl. May play a role in regulating the function of Cbl and its associated protein kinases. Acts as negative regulator of T cell receptor (TCR) signaling. Dephosphorylates and inactivates the SRC family kinases. Belongs to the protein-tyrosine phosphatase family.
序列:
MDQREILQKFLDEAQSKKITKEEFANEFLKLKRQSTKYKADKTYPTTVAEKPKNIKKNRYKDILPYDYSRVELSLITSDEDSSYINANFIKGVYGPKAYIATQGPLSTTLLDFWRMIWEYSVLIIVMACMEYEMGKKKCERYWAEPGEMQLEFGPFSVSCEAEKRKSDYIIRTLKVKFNSETRTIYQFHYKNWPDHDVPSSIDPILELIWDVRCYQEDDSVPICIHCSAGCGRTGVICAIDYTWMLLKDGIIPENFSVFSLIREMRTQRPSLVQTQEQYELVYNAVLELFKRQMDVIRDKHSGTESQAKHCIPEKNHTLQADSYSPNLPKSTTKAAKMMNQQRTKMEIKESSSFDFRTSEISAKEELVLHPAKSSTSFDFLELNYSFDKNADTTMKWQTKAFPIVGEPLQKHQSLDLGSLLFEGCSNSKPVNAAGRYFNSKVPITRTKSTPFELIQQRETKEVDSKENFSYLESQPHDSCFVEMQAQKVMHVSSAELNYSLPYDSKHQIRNASNVKHHDSSALGVYSYIPLVENPYFSSWPPSGTSSKMSLDLPEKQDGTVFPSSLLPTSSTSLFSYYNSHDSLSLNSPTNISSLLNQESAVLATAPRIDDEIPPPLPVRTPESFIVVEEAGEFSPNVPKSLSSAVKVKIGTSLEWGGTSEPKKFDDSVILRPSKSVKLRSPKSELHQDRSSPPPPLPERTLESFFLADEDCMQAQSIETYSTSYPDTMENSTSSKQTLKTPGKSFTRSKSLKILRNMKKSICNSCPPNKPAESVQSNNSSSFLNFGFANRFSKPKGPRNPPPTWNI

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Horse
100
Bovine
100
Rabbit
100
Sheep
92
Dog
92
Chicken
92
Zebrafish
83
Pig
0
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q9Y2R2 作为底物

Site PTM Type Enzyme
K9 Ubiquitination
K17 Ubiquitination
K18 Ubiquitination
K21 Ubiquitination
K30 Ubiquitination
K32 Ubiquitination
S35 Phosphorylation Q05655 (PRKCD)
K51 Ubiquitination
K61 Ubiquitination
Y84 Phosphorylation
K166 Ubiquitination
T173 Phosphorylation
K300 Ubiquitination
K309 Ubiquitination
K315 Ubiquitination
S325 Phosphorylation
K330 Ubiquitination
K337 Ubiquitination
K364 Acetylation
K364 Ubiquitination
K373 Acetylation
K396 Ubiquitination
K400 Ubiquitination
K411 Ubiquitination
S414 Phosphorylation
K429 Ubiquitination
K441 Ubiquitination
T447 Phosphorylation
K448 Ubiquitination
S449 Phosphorylation
K461 Ubiquitination
K466 Ubiquitination
Y499 Phosphorylation
S500 Phosphorylation
K506 Ubiquitination
Y526 Phosphorylation
S527 Phosphorylation
Y528 Phosphorylation
Y536 Phosphorylation
S550 Phosphorylation
K640 Ubiquitination
K647 Ubiquitination
K649 Ubiquitination
S653 Phosphorylation
K663 Ubiquitination
K664 Ubiquitination
K675 Ubiquitination
S691 Phosphorylation
S692 Phosphorylation
T741 Phosphorylation
K744 Ubiquitination
T747 Phosphorylation
S751 Phosphorylation
K770 Ubiquitination

研究背景

功能:

Acts as negative regulator of T-cell receptor (TCR) signaling by direct dephosphorylation of the Src family kinases LCK and FYN, ITAMs of the TCRz/CD3 complex, as well as ZAP70, VAV, VCP and other key signaling molecules. Associates with and probably dephosphorylates CBL. Dephosphorylates LCK at its activating 'Tyr-394' residue. Dephosphorylates ZAP70 at its activating 'Tyr-493' residue. Dephosphorylates the immune system activator SKAP2. Positively regulates toll-like receptor (TLR)-induced type 1 interferon production. Promotes host antiviral responses mediated by type 1 interferon (By similarity). Regulates NOD2-induced pro-inflammatory cytokine secretion and autophagy.

翻译修饰:

Phosphorylation on Ser-35 by PKC/PRKCD abrogates its ability to dephosphorylate and inactivate the SRC family kinases.

细胞定位:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed in bone marrow, B and T-cells, PBMCs, natural killer cells, monocytes, dendritic cells and neutrophils. Both isoform 1 and 4 are predominantly expressed in lymphoid tissues and cells. Isoform 1 is expressed in thymocytes and both mature B and T-cells.

亚基结构:

Interacts with CSK. Interacts with LPXN (By similarity). Interacts with CBL. Interacts with TRAF3 (via MATH domain); the interaction promotes TRAF3 polyubiquitination.

蛋白家族:

Belongs to the protein-tyrosine phosphatase family. Non-receptor class 4 subfamily.

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