产品: RPL5 抗体
货号: DF3712
描述: Rabbit polyclonal antibody to RPL5
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Bovine, Horse, Dog, Chicken, Xenopus
分子量: 34 KD; 34kD(Calculated).
蛋白号: P46777
RRID: AB_2836076

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Bovine(89%), Horse(100%), Dog(100%), Chicken(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
RPL5 Antibody detects endogenous levels of total RPL5.
RRID:
AB_2836076
引用格式: Affinity Biosciences Cat# DF3712, RRID:AB_2836076.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

60S ribosomal protein L5; DBA6; L5; MSTP030; PPP1R135; protein phosphatase 1, regulatory subunit 135; Ribosomal protein L5; RL5; RL5_HUMAN; RP L5; RPL 5; RPL5; U21 RNA; U21RNA;

抗原和靶标

免疫原:

A synthesized peptide derived from human RPL5, corresponding to a region within C-terminal amino acids.

Uniprot:
基因/基因ID:
序列:
MGFVKVVKNKAYFKRYQVKFRRRREGKTDYYARKRLVIQDKNKYNTPKYRMIVRVTNRDIICQIAYARIEGDMIVCAAYAHELPKYGVKVGLTNYAAAYCTGLLLARRLLNRFGMDKIYEGQVEVTGDEYNVESIDGQPGAFTCYLDAGLARTTTGNKVFGALKGAVDGGLSIPHSTKRFPGYDSESKEFNAEVHRKHIMGQNVADYMRYLMEEDEDAYKKQFSQYIKNSVTPDMMEEMYKKAHAAIRENPVYEKKPKKEVKKKRWNRPKMSLAQKKDRVAQKKASFLRAQERAAES

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Horse
100
Dog
100
Xenopus
100
Chicken
100
Bovine
89
Pig
0
Sheep
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P46777 作为底物

Site PTM Type Enzyme
M1 Acetylation
G2 Acetylation
K5 Acetylation
K5 Methylation
K5 Ubiquitination
K19 Ubiquitination
K27 Acetylation
K27 Ubiquitination
Y30 Phosphorylation
Y31 Phosphorylation
K41 Acetylation
K41 Ubiquitination
K43 Acetylation
K43 Ubiquitination
K48 Acetylation
K48 Ubiquitination
Y66 Phosphorylation
C76 S-Nitrosylation
C100 S-Nitrosylation
K158 Ubiquitination
K164 Methylation
K164 Sumoylation
K164 Ubiquitination
S172 Phosphorylation
S176 Phosphorylation
K178 Acetylation
K178 Ubiquitination
Y183 Phosphorylation
S185 Phosphorylation
S187 Phosphorylation
K188 Sumoylation
K188 Ubiquitination
K197 Acetylation
K197 Sumoylation
K197 Ubiquitination
Y207 Phosphorylation
R209 Methylation
Y219 Phosphorylation
K220 Acetylation
K220 Sumoylation
K220 Ubiquitination
K221 Acetylation
K221 Sumoylation
K221 Ubiquitination
K228 Methylation
K228 Ubiquitination
S230 Phosphorylation
T232 Phosphorylation
Y240 Phosphorylation
K241 Acetylation
K242 Acetylation
K242 Ubiquitination
R248 Methylation
Y253 Phosphorylation
K270 Ubiquitination
S272 Phosphorylation
K276 Acetylation
K283 Methylation
K284 Ubiquitination
S286 Phosphorylation

研究背景

功能:

Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel. As part of the 5S RNP/5S ribonucleoprotein particle it is an essential component of the LSU, required for its formation and the maturation of rRNAs. It also couples ribosome biogenesis to p53/TP53 activation. As part of the 5S RNP it accumulates in the nucleoplasm and inhibits MDM2, when ribosome biogenesis is perturbed, mediating the stabilization and the activation of TP53.

细胞定位:

Cytoplasm. Nucleus>Nucleolus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Component of the large ribosomal subunit (LSU). Part of a LSU subcomplex, the 5S RNP which is composed of the 5S RNA, RPL5 and RPL11. Interacts with isoform 1 of NVL in an ATP-dependent manner. Interacts with RRP1B.

蛋白家族:

Belongs to the universal ribosomal protein uL18 family.

研究领域

· Genetic Information Processing > Translation > Ribosome.

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